2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthe...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n9_p2806_Pontiggia |
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todo:paper_0960894X_v20_n9_p2806_Pontiggia2023-10-03T15:54:01Z 2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor Pontiggia, R. Pontiggia, O. Simian, M. Montserrat, J.M. Engels, J.W. Iribarren, A.M. 2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented. © 2010 Elsevier Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n9_p2806_Pontiggia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine |
| spellingShingle |
2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine Pontiggia, R. Pontiggia, O. Simian, M. Montserrat, J.M. Engels, J.W. Iribarren, A.M. 2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor |
| topic_facet |
2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine |
| description |
A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented. © 2010 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Pontiggia, R. Pontiggia, O. Simian, M. Montserrat, J.M. Engels, J.W. Iribarren, A.M. |
| author_facet |
Pontiggia, R. Pontiggia, O. Simian, M. Montserrat, J.M. Engels, J.W. Iribarren, A.M. |
| author_sort |
Pontiggia, R. |
| title |
2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor |
| title_short |
2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor |
| title_full |
2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor |
| title_fullStr |
2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor |
| title_full_unstemmed |
2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor |
| title_sort |
2-c-methyluridine modified hammerhead ribozyme against the estrogen receptor |
| url |
http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n9_p2806_Pontiggia |
| work_keys_str_mv |
AT pontiggiar 2cmethyluridinemodifiedhammerheadribozymeagainsttheestrogenreceptor AT pontiggiao 2cmethyluridinemodifiedhammerheadribozymeagainsttheestrogenreceptor AT simianm 2cmethyluridinemodifiedhammerheadribozymeagainsttheestrogenreceptor AT montserratjm 2cmethyluridinemodifiedhammerheadribozymeagainsttheestrogenreceptor AT engelsjw 2cmethyluridinemodifiedhammerheadribozymeagainsttheestrogenreceptor AT iribarrenam 2cmethyluridinemodifiedhammerheadribozymeagainsttheestrogenreceptor |
| _version_ |
1782026607458779136 |