10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core
The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Re...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n15_p4367_Robaldo |
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todo:paper_0960894X_v20_n15_p4367_Robaldo2023-10-03T15:54:00Z 10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core Robaldo, L. Montserrat, J.M. Iribarren, A.M. 10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2′-C-methyl-2′-deoxynucleosides increased the stability. © 2010 Elsevier Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n15_p4367_Robaldo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine |
| spellingShingle |
10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine Robaldo, L. Montserrat, J.M. Iribarren, A.M. 10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| topic_facet |
10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine |
| description |
The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2′-C-methyl-2′-deoxynucleosides increased the stability. © 2010 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Robaldo, L. Montserrat, J.M. Iribarren, A.M. |
| author_facet |
Robaldo, L. Montserrat, J.M. Iribarren, A.M. |
| author_sort |
Robaldo, L. |
| title |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_short |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_full |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_fullStr |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_full_unstemmed |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_sort |
10-23 dnazyme modified with (2′r)- and (2′s)-2′-deoxy- 2′-c-methyluridine in the catalytic core |
| url |
http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n15_p4367_Robaldo |
| work_keys_str_mv |
AT robaldol 1023dnazymemodifiedwith2rand2s2deoxy2cmethyluridineinthecatalyticcore AT montserratjm 1023dnazymemodifiedwith2rand2s2deoxy2cmethyluridineinthecatalyticcore AT iribarrenam 1023dnazymemodifiedwith2rand2s2deoxy2cmethyluridineinthecatalyticcore |
| _version_ |
1807321234250137600 |