Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling

In the work reported in this paper, we attempted to elucidate the nature of the different abscisic acid (ABA) sensitivities presented by developing embryos from sorghum [Sorghum bicolor (L.) Moench] lines with contrasting pre-harvest sprouting (PHS) behaviour (Redland B2, susceptible; IS 9530, resis...

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Detalles Bibliográficos
Autores principales: Gualano, N., Carrari, F., Rodríguez, M.V., Pérez-Flores, L., Sánchez, R., Iusem, N., Benech-Arnold, R.
Formato: JOUR
Materias:
Abscisic acid
Abscisic acid sensitivity
Abscisic acid signalling
Germination
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09602585_v17_n2_p81_Gualano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09602585_v17_n2_p81_Gualano
record_format dspace
spelling todo:paper_09602585_v17_n2_p81_Gualano2023-10-03T15:53:42Z Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling Gualano, N. Carrari, F. Rodríguez, M.V. Pérez-Flores, L. Sánchez, R. Iusem, N. Benech-Arnold, R. Abscisic acid Abscisic acid sensitivity Abscisic acid signalling Germination Pre-harvest sprouting Seed dormancy Sorghum bicolor Sorghum bicolor In the work reported in this paper, we attempted to elucidate the nature of the different abscisic acid (ABA) sensitivities presented by developing embryos from sorghum [Sorghum bicolor (L.) Moench] lines with contrasting pre-harvest sprouting (PHS) behaviour (Redland B2, susceptible; IS 9530, resistant). We explored two different hypotheses for a possible mechanism: (1) a different functionality of the ABA signalling pathway, and (2) a different rate of ABA degradation/conjugation in the apoplast of embryos from these genotypes. To assess the first possibility, we used an ABA-responsive gene (Rab17) as a reporter of changes in endogenous ABA content, which were artificially induced in embryos from both genotypes by means of fluridone application immediately after anthesis, to reduce ABA content, and embryo incubation in the presence of ABA. A defect in ABA signalling should be seen as a level of Rab17 expression that is independent of endogenous ABA content. For testing the second possibility at two stages of development, embryos from both lines were isolated and incubated in water for different periods. ABA concentrations in embryos and the incubation media were quantified through radioimmunoassay. In contrast to our findings for the resistant IS 9530 line, Rab17 expression did not respond to changes in ABA levels in sensitive Redland B2 embryos. The ABA degradation/conjugation rates in embryos and incubation media did not show clear differences between sorghum lines for any of the developmental stages analysed. These results suggest that a disruption in the ABA signal transduction pathway in Redland B2 underlies the low ABA sensitivity shown by embryos from this line. © 2007 Cambridge University Press. Fil:Carrari, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09602585_v17_n2_p81_Gualano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Abscisic acid
Abscisic acid sensitivity
Abscisic acid signalling
Germination
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
Sorghum bicolor
spellingShingle Abscisic acid
Abscisic acid sensitivity
Abscisic acid signalling
Germination
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
Sorghum bicolor
Gualano, N.
Carrari, F.
Rodríguez, M.V.
Pérez-Flores, L.
Sánchez, R.
Iusem, N.
Benech-Arnold, R.
Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling
topic_facet Abscisic acid
Abscisic acid sensitivity
Abscisic acid signalling
Germination
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
Sorghum bicolor
description In the work reported in this paper, we attempted to elucidate the nature of the different abscisic acid (ABA) sensitivities presented by developing embryos from sorghum [Sorghum bicolor (L.) Moench] lines with contrasting pre-harvest sprouting (PHS) behaviour (Redland B2, susceptible; IS 9530, resistant). We explored two different hypotheses for a possible mechanism: (1) a different functionality of the ABA signalling pathway, and (2) a different rate of ABA degradation/conjugation in the apoplast of embryos from these genotypes. To assess the first possibility, we used an ABA-responsive gene (Rab17) as a reporter of changes in endogenous ABA content, which were artificially induced in embryos from both genotypes by means of fluridone application immediately after anthesis, to reduce ABA content, and embryo incubation in the presence of ABA. A defect in ABA signalling should be seen as a level of Rab17 expression that is independent of endogenous ABA content. For testing the second possibility at two stages of development, embryos from both lines were isolated and incubated in water for different periods. ABA concentrations in embryos and the incubation media were quantified through radioimmunoassay. In contrast to our findings for the resistant IS 9530 line, Rab17 expression did not respond to changes in ABA levels in sensitive Redland B2 embryos. The ABA degradation/conjugation rates in embryos and incubation media did not show clear differences between sorghum lines for any of the developmental stages analysed. These results suggest that a disruption in the ABA signal transduction pathway in Redland B2 underlies the low ABA sensitivity shown by embryos from this line. © 2007 Cambridge University Press.
format JOUR
author Gualano, N.
Carrari, F.
Rodríguez, M.V.
Pérez-Flores, L.
Sánchez, R.
Iusem, N.
Benech-Arnold, R.
author_facet Gualano, N.
Carrari, F.
Rodríguez, M.V.
Pérez-Flores, L.
Sánchez, R.
Iusem, N.
Benech-Arnold, R.
author_sort Gualano, N.
title Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling
title_short Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling
title_full Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling
title_fullStr Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling
title_full_unstemmed Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling
title_sort reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (sorghum bicolor) variety is associated with altered aba signalling
url http://hdl.handle.net/20.500.12110/paper_09602585_v17_n2_p81_Gualano
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