Basis of progesterone protection in spinal cord neurodegeneration
Progesterone neuroprotection has been reported in experimental brain, peripheral nerve and spinal cord injury. To investigate for a similar role in neurodegeneration, we studied progesterone effects in the Wobbler mouse, a mutant presenting severe motoneuron degeneration and astrogliosis of the spin...
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todo:paper_09600760_v83_n1-5_p199_GonzalezDeniselle2023-10-03T15:53:32Z Basis of progesterone protection in spinal cord neurodegeneration Gonzalez Deniselle, M.C. Lopez Costa, J.J. Gonzalez, S.L. Labombarda, F. Garay, L. Guennoun, R. Schumacher, M. De Nicola, A.F. Neurodegeneration Progesterone Spinal cord Wobbler mouse adenosine triphosphatase (potassium sodium) glial fibrillary acidic protein messenger RNA neuromodulin progesterone adenosine triphosphatase cation transport protein glial fibrillary acidic protein messenger RNA neuromodulin potassium transporting ATPase progesterone alpha chain astrocytosis beta chain brain mitochondrion cell organelle cell ultrastructure cell vacuole conference paper drug implant drug pellet grip strength hormonal regulation hormonal therapy hormone action immunohistochemistry morphological trait mouse strain nerve degeneration neuropathology neuroprotection nonhuman observation progesterone synthesis protein blood level protein determination protein expression species comparison spinal cord motoneuron survival time animal apoptosis article astrocyte cell nucleus chromatin degenerative disease electron microscopy in situ hybridization metabolism mouse nerve cell pathology spinal cord ultrastructure Animalia Adenosine Triphosphatases Animals Apoptosis Astrocytes Cation Transport Proteins Cell Nucleus Chromatin GAP-43 Protein Glial Fibrillary Acidic Protein In Situ Hybridization Mice Microscopy, Electron Neurodegenerative Diseases Neurons Progesterone RNA, Messenger Spinal Cord Progesterone neuroprotection has been reported in experimental brain, peripheral nerve and spinal cord injury. To investigate for a similar role in neurodegeneration, we studied progesterone effects in the Wobbler mouse, a mutant presenting severe motoneuron degeneration and astrogliosis of the spinal cord. Implant of a single progesterone pellet (20mg) during 15 days produced substantial changes in Wobbler mice spinal cord. Morphologically, motoneurons of untreated Wobbler mice showed severe vacuolation of intracellular organelles including mitochondria. In contrast, neuropathology was less pronounced in Wobbler mice receiving progesterone, together with a reduction of vacuolated cells and preservation of mitochondrial ultrastructure. Determination of mRNAs for the α3 and β1 subunits of neuronal Na, K-ATPase, showed that mRNA levels in untreated mice were significantly reduced, whereas progesterone therapy re-established the expression of both subunits. Additionally, progesterone treatment of Wobbler mice attenuated the aberrant expression of the growth-associated protein (GAP-43) mRNA which otherwise occurred in motoneurons of untreated animals. The hormone, however, was without effect on astrocytosis of Wobbler mice, determined by glial fibrillary acidic protein (GFAP)-immunostaining. Lastly, progesterone treatment of Wobbler mice enhanced grip strength and prolonged survival at the end of the 15-day observation period. Recovery of morphology and molecular motoneuron parameters of Wobbler mice receiving progesterone, suggest a new and important role for this hormone in the prevention of spinal cord neurodegenerative disorders. © 2003 Elsevier Science Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09600760_v83_n1-5_p199_GonzalezDeniselle |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Neurodegeneration Progesterone Spinal cord Wobbler mouse adenosine triphosphatase (potassium sodium) glial fibrillary acidic protein messenger RNA neuromodulin progesterone adenosine triphosphatase cation transport protein glial fibrillary acidic protein messenger RNA neuromodulin potassium transporting ATPase progesterone alpha chain astrocytosis beta chain brain mitochondrion cell organelle cell ultrastructure cell vacuole conference paper drug implant drug pellet grip strength hormonal regulation hormonal therapy hormone action immunohistochemistry morphological trait mouse strain nerve degeneration neuropathology neuroprotection nonhuman observation progesterone synthesis protein blood level protein determination protein expression species comparison spinal cord motoneuron survival time animal apoptosis article astrocyte cell nucleus chromatin degenerative disease electron microscopy in situ hybridization metabolism mouse nerve cell pathology spinal cord ultrastructure Animalia Adenosine Triphosphatases Animals Apoptosis Astrocytes Cation Transport Proteins Cell Nucleus Chromatin GAP-43 Protein Glial Fibrillary Acidic Protein In Situ Hybridization Mice Microscopy, Electron Neurodegenerative Diseases Neurons Progesterone RNA, Messenger Spinal Cord |
| spellingShingle |
Neurodegeneration Progesterone Spinal cord Wobbler mouse adenosine triphosphatase (potassium sodium) glial fibrillary acidic protein messenger RNA neuromodulin progesterone adenosine triphosphatase cation transport protein glial fibrillary acidic protein messenger RNA neuromodulin potassium transporting ATPase progesterone alpha chain astrocytosis beta chain brain mitochondrion cell organelle cell ultrastructure cell vacuole conference paper drug implant drug pellet grip strength hormonal regulation hormonal therapy hormone action immunohistochemistry morphological trait mouse strain nerve degeneration neuropathology neuroprotection nonhuman observation progesterone synthesis protein blood level protein determination protein expression species comparison spinal cord motoneuron survival time animal apoptosis article astrocyte cell nucleus chromatin degenerative disease electron microscopy in situ hybridization metabolism mouse nerve cell pathology spinal cord ultrastructure Animalia Adenosine Triphosphatases Animals Apoptosis Astrocytes Cation Transport Proteins Cell Nucleus Chromatin GAP-43 Protein Glial Fibrillary Acidic Protein In Situ Hybridization Mice Microscopy, Electron Neurodegenerative Diseases Neurons Progesterone RNA, Messenger Spinal Cord Gonzalez Deniselle, M.C. Lopez Costa, J.J. Gonzalez, S.L. Labombarda, F. Garay, L. Guennoun, R. Schumacher, M. De Nicola, A.F. Basis of progesterone protection in spinal cord neurodegeneration |
| topic_facet |
Neurodegeneration Progesterone Spinal cord Wobbler mouse adenosine triphosphatase (potassium sodium) glial fibrillary acidic protein messenger RNA neuromodulin progesterone adenosine triphosphatase cation transport protein glial fibrillary acidic protein messenger RNA neuromodulin potassium transporting ATPase progesterone alpha chain astrocytosis beta chain brain mitochondrion cell organelle cell ultrastructure cell vacuole conference paper drug implant drug pellet grip strength hormonal regulation hormonal therapy hormone action immunohistochemistry morphological trait mouse strain nerve degeneration neuropathology neuroprotection nonhuman observation progesterone synthesis protein blood level protein determination protein expression species comparison spinal cord motoneuron survival time animal apoptosis article astrocyte cell nucleus chromatin degenerative disease electron microscopy in situ hybridization metabolism mouse nerve cell pathology spinal cord ultrastructure Animalia Adenosine Triphosphatases Animals Apoptosis Astrocytes Cation Transport Proteins Cell Nucleus Chromatin GAP-43 Protein Glial Fibrillary Acidic Protein In Situ Hybridization Mice Microscopy, Electron Neurodegenerative Diseases Neurons Progesterone RNA, Messenger Spinal Cord |
| description |
Progesterone neuroprotection has been reported in experimental brain, peripheral nerve and spinal cord injury. To investigate for a similar role in neurodegeneration, we studied progesterone effects in the Wobbler mouse, a mutant presenting severe motoneuron degeneration and astrogliosis of the spinal cord. Implant of a single progesterone pellet (20mg) during 15 days produced substantial changes in Wobbler mice spinal cord. Morphologically, motoneurons of untreated Wobbler mice showed severe vacuolation of intracellular organelles including mitochondria. In contrast, neuropathology was less pronounced in Wobbler mice receiving progesterone, together with a reduction of vacuolated cells and preservation of mitochondrial ultrastructure. Determination of mRNAs for the α3 and β1 subunits of neuronal Na, K-ATPase, showed that mRNA levels in untreated mice were significantly reduced, whereas progesterone therapy re-established the expression of both subunits. Additionally, progesterone treatment of Wobbler mice attenuated the aberrant expression of the growth-associated protein (GAP-43) mRNA which otherwise occurred in motoneurons of untreated animals. The hormone, however, was without effect on astrocytosis of Wobbler mice, determined by glial fibrillary acidic protein (GFAP)-immunostaining. Lastly, progesterone treatment of Wobbler mice enhanced grip strength and prolonged survival at the end of the 15-day observation period. Recovery of morphology and molecular motoneuron parameters of Wobbler mice receiving progesterone, suggest a new and important role for this hormone in the prevention of spinal cord neurodegenerative disorders. © 2003 Elsevier Science Ltd. All rights reserved. |
| format |
JOUR |
| author |
Gonzalez Deniselle, M.C. Lopez Costa, J.J. Gonzalez, S.L. Labombarda, F. Garay, L. Guennoun, R. Schumacher, M. De Nicola, A.F. |
| author_facet |
Gonzalez Deniselle, M.C. Lopez Costa, J.J. Gonzalez, S.L. Labombarda, F. Garay, L. Guennoun, R. Schumacher, M. De Nicola, A.F. |
| author_sort |
Gonzalez Deniselle, M.C. |
| title |
Basis of progesterone protection in spinal cord neurodegeneration |
| title_short |
Basis of progesterone protection in spinal cord neurodegeneration |
| title_full |
Basis of progesterone protection in spinal cord neurodegeneration |
| title_fullStr |
Basis of progesterone protection in spinal cord neurodegeneration |
| title_full_unstemmed |
Basis of progesterone protection in spinal cord neurodegeneration |
| title_sort |
basis of progesterone protection in spinal cord neurodegeneration |
| url |
http://hdl.handle.net/20.500.12110/paper_09600760_v83_n1-5_p199_GonzalezDeniselle |
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| _version_ |
1807315948627034112 |