Regulation of gene expression by corticoid hormones in the brain and spinal cord
Glucocorticoids (GC) and mineralocorticoids (MC) have profound regulatory effects upon the central nervous system (CNS). Hormonal regulation affects several molecules essential to CNS function. First, evidences are presented that mRNA expression of the α3 and β1-subunits of the Na,K- ATPase are incr...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09600760_v65_n1-6_p253_DeNicola |
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todo:paper_09600760_v65_n1-6_p253_DeNicola2023-10-03T15:53:31Z Regulation of gene expression by corticoid hormones in the brain and spinal cord De Nicola, A.F. Ferrini, M. Gonzalez, S.L. Gonzalez Deniselle, M.C. Grillo, C.A. Piroli, G. Saravia, F. De Kloet, E.R. adenosine triphosphatase (potassium sodium) aldosterone argipressin corticosteroid dexamethasone glial fibrillary acidic protein messenger rna nerve growth factor receptor neurotrophin animal cell animal tissue central nervous system conference paper gene expression regulation hormonal regulation immunoreactivity neuropathology nonhuman rat regulatory mechanism sex difference spinal cord transsection Adrenal Cortex Hormones Animals Arginine Vasopressin Brain Female Gene Expression Regulation Glial Fibrillary Acidic Protein Male Na(+)-K(+)-Exchanging ATPase Rats Sex Characteristics Spinal Cord Animalia Eukaryota Glucocorticoids (GC) and mineralocorticoids (MC) have profound regulatory effects upon the central nervous system (CNS). Hormonal regulation affects several molecules essential to CNS function. First, evidences are presented that mRNA expression of the α3 and β1-subunits of the Na,K- ATPase are increased by GC and physiological doses of MC in a region- dependent manner. Instead, high MC doses reduce the β1 isoform and enzyme activity in amygdaloid and hypothalamic nuclei, an effect which may be related to MC control of salt appetite. The α3-subunit mRNA of the Na,K- ATPase is also stimulated by GC in motoneurons of the injured spinal cord, suggesting a role for the enzyme in GC neuroprotection. Second, we provide evidences for hormonal effects on the expression of mRNA for the neuropeptide arginine vasopressin (AVP). Our data show that GC inhibition of AVP mRNA levels in the paraventricular nucleus is sex-hormone dependent. This sexual dimorphism may explain sex differences in the hypothalamic-pituitary-adrenal axis function between female and male rats. Third, steroid effects on the astrocyte marker glial fibrillary acidic protein (GFAP) points to a complex regulatory mechanism. In an animal model of neurodegeneration (the Wobbler mouse) showing pronounced astrogliosis of the spinal cord, in vivo GC treatment down-regulated GFAP immunoreactivity, whereas the membrane-active steroid antioxidant U74389F up-regulated this protein. It is likely that variations in GFAP protein expression affect spinal cord neurodegeneration in Wobbler mice. Fourth, an interaction between neurotrophins and GC is shown in the injured rat spinal cord. In this model, intensive GC treatment increases immunoreactive low affinity nerve growth factor (NGF) receptor in motoneuron processes. Because GC also increases immunoreactive NGF, this mechanism would support trophism and regeneration in damaged tissues. In conclusion, evidences show that some molecules regulated by adrenal steroids in neurons and glial cells are not only involved in physiological control, but additionally, may play important roles in neuropathology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09600760_v65_n1-6_p253_DeNicola |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenosine triphosphatase (potassium sodium) aldosterone argipressin corticosteroid dexamethasone glial fibrillary acidic protein messenger rna nerve growth factor receptor neurotrophin animal cell animal tissue central nervous system conference paper gene expression regulation hormonal regulation immunoreactivity neuropathology nonhuman rat regulatory mechanism sex difference spinal cord transsection Adrenal Cortex Hormones Animals Arginine Vasopressin Brain Female Gene Expression Regulation Glial Fibrillary Acidic Protein Male Na(+)-K(+)-Exchanging ATPase Rats Sex Characteristics Spinal Cord Animalia Eukaryota |
| spellingShingle |
adenosine triphosphatase (potassium sodium) aldosterone argipressin corticosteroid dexamethasone glial fibrillary acidic protein messenger rna nerve growth factor receptor neurotrophin animal cell animal tissue central nervous system conference paper gene expression regulation hormonal regulation immunoreactivity neuropathology nonhuman rat regulatory mechanism sex difference spinal cord transsection Adrenal Cortex Hormones Animals Arginine Vasopressin Brain Female Gene Expression Regulation Glial Fibrillary Acidic Protein Male Na(+)-K(+)-Exchanging ATPase Rats Sex Characteristics Spinal Cord Animalia Eukaryota De Nicola, A.F. Ferrini, M. Gonzalez, S.L. Gonzalez Deniselle, M.C. Grillo, C.A. Piroli, G. Saravia, F. De Kloet, E.R. Regulation of gene expression by corticoid hormones in the brain and spinal cord |
| topic_facet |
adenosine triphosphatase (potassium sodium) aldosterone argipressin corticosteroid dexamethasone glial fibrillary acidic protein messenger rna nerve growth factor receptor neurotrophin animal cell animal tissue central nervous system conference paper gene expression regulation hormonal regulation immunoreactivity neuropathology nonhuman rat regulatory mechanism sex difference spinal cord transsection Adrenal Cortex Hormones Animals Arginine Vasopressin Brain Female Gene Expression Regulation Glial Fibrillary Acidic Protein Male Na(+)-K(+)-Exchanging ATPase Rats Sex Characteristics Spinal Cord Animalia Eukaryota |
| description |
Glucocorticoids (GC) and mineralocorticoids (MC) have profound regulatory effects upon the central nervous system (CNS). Hormonal regulation affects several molecules essential to CNS function. First, evidences are presented that mRNA expression of the α3 and β1-subunits of the Na,K- ATPase are increased by GC and physiological doses of MC in a region- dependent manner. Instead, high MC doses reduce the β1 isoform and enzyme activity in amygdaloid and hypothalamic nuclei, an effect which may be related to MC control of salt appetite. The α3-subunit mRNA of the Na,K- ATPase is also stimulated by GC in motoneurons of the injured spinal cord, suggesting a role for the enzyme in GC neuroprotection. Second, we provide evidences for hormonal effects on the expression of mRNA for the neuropeptide arginine vasopressin (AVP). Our data show that GC inhibition of AVP mRNA levels in the paraventricular nucleus is sex-hormone dependent. This sexual dimorphism may explain sex differences in the hypothalamic-pituitary-adrenal axis function between female and male rats. Third, steroid effects on the astrocyte marker glial fibrillary acidic protein (GFAP) points to a complex regulatory mechanism. In an animal model of neurodegeneration (the Wobbler mouse) showing pronounced astrogliosis of the spinal cord, in vivo GC treatment down-regulated GFAP immunoreactivity, whereas the membrane-active steroid antioxidant U74389F up-regulated this protein. It is likely that variations in GFAP protein expression affect spinal cord neurodegeneration in Wobbler mice. Fourth, an interaction between neurotrophins and GC is shown in the injured rat spinal cord. In this model, intensive GC treatment increases immunoreactive low affinity nerve growth factor (NGF) receptor in motoneuron processes. Because GC also increases immunoreactive NGF, this mechanism would support trophism and regeneration in damaged tissues. In conclusion, evidences show that some molecules regulated by adrenal steroids in neurons and glial cells are not only involved in physiological control, but additionally, may play important roles in neuropathology. |
| format |
JOUR |
| author |
De Nicola, A.F. Ferrini, M. Gonzalez, S.L. Gonzalez Deniselle, M.C. Grillo, C.A. Piroli, G. Saravia, F. De Kloet, E.R. |
| author_facet |
De Nicola, A.F. Ferrini, M. Gonzalez, S.L. Gonzalez Deniselle, M.C. Grillo, C.A. Piroli, G. Saravia, F. De Kloet, E.R. |
| author_sort |
De Nicola, A.F. |
| title |
Regulation of gene expression by corticoid hormones in the brain and spinal cord |
| title_short |
Regulation of gene expression by corticoid hormones in the brain and spinal cord |
| title_full |
Regulation of gene expression by corticoid hormones in the brain and spinal cord |
| title_fullStr |
Regulation of gene expression by corticoid hormones in the brain and spinal cord |
| title_full_unstemmed |
Regulation of gene expression by corticoid hormones in the brain and spinal cord |
| title_sort |
regulation of gene expression by corticoid hormones in the brain and spinal cord |
| url |
http://hdl.handle.net/20.500.12110/paper_09600760_v65_n1-6_p253_DeNicola |
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