Effects of juvenile hormone on mammalian steroidogenesis

The effects of juvenile hormone-III (JH-III) and the JH analogue 2-(4-phenoxyphenoxy)-ethoxytetrahydropiran on testicular steroidogenesis were studied. By using cultured MA-10 Leydig tumor cells as a model, these compounds were found to be potent inhibitors of LH/hCG steroidogenic action in a dose-d...

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Detalles Bibliográficos
Autores principales: Vladusic, E.A., Bussmann, L.E., Visconti, P.E., Stoka, A.M., Rodriguez, J.B., Gros, E.G., Charreau, E.H.
Formato: JOUR
Materias:
adenylate cyclase
cyclic amp
juvenile hormone
juvenile hormone derivative
article
cell culture
leydig cell tumor
nonhuman
progesterone synthesis
steroidogenesis
testis
Animal
Cell Line
Cholera Toxin
Chorionic Gonadotropin
Cyclic AMP
Guanosine Triphosphate
Juvenile Hormones
Leydig Cells
Male
Mammals
Progesterone
Signal Transduction
Support, Non-U.S. Gov't
Mammalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09600760_v50_n3-4_p181_Vladusic
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09600760_v50_n3-4_p181_Vladusic
record_format dspace
spelling todo:paper_09600760_v50_n3-4_p181_Vladusic2023-10-03T15:53:28Z Effects of juvenile hormone on mammalian steroidogenesis Vladusic, E.A. Bussmann, L.E. Visconti, P.E. Stoka, A.M. Rodriguez, J.B. Gros, E.G. Charreau, E.H. adenylate cyclase cyclic amp juvenile hormone juvenile hormone derivative article cell culture leydig cell tumor nonhuman progesterone synthesis steroidogenesis testis Animal Cell Line Cholera Toxin Chorionic Gonadotropin Cyclic AMP Guanosine Triphosphate Juvenile Hormones Leydig Cells Male Mammals Progesterone Signal Transduction Support, Non-U.S. Gov't Mammalia The effects of juvenile hormone-III (JH-III) and the JH analogue 2-(4-phenoxyphenoxy)-ethoxytetrahydropiran on testicular steroidogenesis were studied. By using cultured MA-10 Leydig tumor cells as a model, these compounds were found to be potent inhibitors of LH/hCG steroidogenic action in a dose-dependent manner. Scatchard plot analysis of the binding data indicated that the JH analogue did not significantly alter the affinity nor the number of hCG binding sites, as well as GTP binding to plasma membranes. JH analogue inhibited the stimulatory action of both cholera toxin and forskolin on cAMP production and the concomitant steroidogenic response. JH analogue inhibited (Bu) 2 cAMP-stimulated progesterone synthesis, indicating that a process downstream to the adenylyl cyclase in the steroidogenic pathway is also affected. © 1994. Fil:Vladusic, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stoka, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, J.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gros, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09600760_v50_n3-4_p181_Vladusic
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adenylate cyclase
cyclic amp
juvenile hormone
juvenile hormone derivative
article
cell culture
leydig cell tumor
nonhuman
progesterone synthesis
steroidogenesis
testis
Animal
Cell Line
Cholera Toxin
Chorionic Gonadotropin
Cyclic AMP
Guanosine Triphosphate
Juvenile Hormones
Leydig Cells
Male
Mammals
Progesterone
Signal Transduction
Support, Non-U.S. Gov't
Mammalia
spellingShingle adenylate cyclase
cyclic amp
juvenile hormone
juvenile hormone derivative
article
cell culture
leydig cell tumor
nonhuman
progesterone synthesis
steroidogenesis
testis
Animal
Cell Line
Cholera Toxin
Chorionic Gonadotropin
Cyclic AMP
Guanosine Triphosphate
Juvenile Hormones
Leydig Cells
Male
Mammals
Progesterone
Signal Transduction
Support, Non-U.S. Gov't
Mammalia
Vladusic, E.A.
Bussmann, L.E.
Visconti, P.E.
Stoka, A.M.
Rodriguez, J.B.
Gros, E.G.
Charreau, E.H.
Effects of juvenile hormone on mammalian steroidogenesis
topic_facet adenylate cyclase
cyclic amp
juvenile hormone
juvenile hormone derivative
article
cell culture
leydig cell tumor
nonhuman
progesterone synthesis
steroidogenesis
testis
Animal
Cell Line
Cholera Toxin
Chorionic Gonadotropin
Cyclic AMP
Guanosine Triphosphate
Juvenile Hormones
Leydig Cells
Male
Mammals
Progesterone
Signal Transduction
Support, Non-U.S. Gov't
Mammalia
description The effects of juvenile hormone-III (JH-III) and the JH analogue 2-(4-phenoxyphenoxy)-ethoxytetrahydropiran on testicular steroidogenesis were studied. By using cultured MA-10 Leydig tumor cells as a model, these compounds were found to be potent inhibitors of LH/hCG steroidogenic action in a dose-dependent manner. Scatchard plot analysis of the binding data indicated that the JH analogue did not significantly alter the affinity nor the number of hCG binding sites, as well as GTP binding to plasma membranes. JH analogue inhibited the stimulatory action of both cholera toxin and forskolin on cAMP production and the concomitant steroidogenic response. JH analogue inhibited (Bu) 2 cAMP-stimulated progesterone synthesis, indicating that a process downstream to the adenylyl cyclase in the steroidogenic pathway is also affected. © 1994.
format JOUR
author Vladusic, E.A.
Bussmann, L.E.
Visconti, P.E.
Stoka, A.M.
Rodriguez, J.B.
Gros, E.G.
Charreau, E.H.
author_facet Vladusic, E.A.
Bussmann, L.E.
Visconti, P.E.
Stoka, A.M.
Rodriguez, J.B.
Gros, E.G.
Charreau, E.H.
author_sort Vladusic, E.A.
title Effects of juvenile hormone on mammalian steroidogenesis
title_short Effects of juvenile hormone on mammalian steroidogenesis
title_full Effects of juvenile hormone on mammalian steroidogenesis
title_fullStr Effects of juvenile hormone on mammalian steroidogenesis
title_full_unstemmed Effects of juvenile hormone on mammalian steroidogenesis
title_sort effects of juvenile hormone on mammalian steroidogenesis
url http://hdl.handle.net/20.500.12110/paper_09600760_v50_n3-4_p181_Vladusic
work_keys_str_mv AT vladusicea effectsofjuvenilehormoneonmammaliansteroidogenesis
AT bussmannle effectsofjuvenilehormoneonmammaliansteroidogenesis
AT viscontipe effectsofjuvenilehormoneonmammaliansteroidogenesis
AT stokaam effectsofjuvenilehormoneonmammaliansteroidogenesis
AT rodriguezjb effectsofjuvenilehormoneonmammaliansteroidogenesis
AT groseg effectsofjuvenilehormoneonmammaliansteroidogenesis
AT charreaueh effectsofjuvenilehormoneonmammaliansteroidogenesis
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