Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion

The immobilization of mammalian cells within semipermeable matrices which allow retention of viability and functionality has recently attracted research interest. In this study, rat mature ovarian follicles were encapsulated in sol-gel silica matrices for the evaluation of in vitro steroid hormones...

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Autores principales: Catalano, P.N., Bourguignon, N.S., Alvarez, G.S., Libertun, C., Diaz, L.E., Desimone, M.F., Lux-Lantos, V.
Formato: JOUR
Materias:
Adverse effect
Cell structure
Cell types
Cell viability
Cell-based
Delivery systems
In-vitro
Mammalian cells
Orthosilicate
Ovarian follicles
Proliferation rate
Silica matrix
Sol-gel silica
Steroid hormone
Tetraethoxysilanes
Tetrakis
Turner syndrome
Cells
Gynecology
Physiology
Silica
Sol-gel process
Sol-gels
Cytology
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09599428_v22_n23_p11681_Catalano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09599428_v22_n23_p11681_Catalano
record_format dspace
spelling todo:paper_09599428_v22_n23_p11681_Catalano2023-10-03T15:53:19Z Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion Catalano, P.N. Bourguignon, N.S. Alvarez, G.S. Libertun, C. Diaz, L.E. Desimone, M.F. Lux-Lantos, V. Adverse effect Cell structure Cell types Cell viability Cell-based Delivery systems In-vitro Mammalian cells Orthosilicate Ovarian follicles Proliferation rate Silica matrix Sol-gel silica Steroid hormone Tetraethoxysilanes Tetrakis Turner syndrome Cells Gynecology Physiology Silica Sol-gel process Sol-gels Cytology The immobilization of mammalian cells within semipermeable matrices which allow retention of viability and functionality has recently attracted research interest. In this study, rat mature ovarian follicles were encapsulated in sol-gel silica matrices for the evaluation of in vitro steroid hormones production. Tetraethoxysilane (TEOS) and tetrakis(2-hydroxyethyl)orthosilicate (THEOS) were evaluated for cell viability, progesterone and estradiol secretion and cell structure conservation. Significant differences were observed between TEOS and THEOS, which allowed encapsulation of follicles for 9 days. Although encapsulated follicles showed lower viability and proliferation rates than control follicles, they were able to secrete estradiol to the same extent as control follicles. This study demonstrated an effective collaboration between two different mammalian cell types in hormone production and secretion when encapsulated in silica matrices. These results could lead to the development of ovarian cell-based hormone delivery systems for the treatment of the symptoms of menopause and the Turner syndrome minimizing adverse effects. © 2012 The Royal Society of Chemistry. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09599428_v22_n23_p11681_Catalano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adverse effect
Cell structure
Cell types
Cell viability
Cell-based
Delivery systems
In-vitro
Mammalian cells
Orthosilicate
Ovarian follicles
Proliferation rate
Silica matrix
Sol-gel silica
Steroid hormone
Tetraethoxysilanes
Tetrakis
Turner syndrome
Cells
Gynecology
Physiology
Silica
Sol-gel process
Sol-gels
Cytology
spellingShingle Adverse effect
Cell structure
Cell types
Cell viability
Cell-based
Delivery systems
In-vitro
Mammalian cells
Orthosilicate
Ovarian follicles
Proliferation rate
Silica matrix
Sol-gel silica
Steroid hormone
Tetraethoxysilanes
Tetrakis
Turner syndrome
Cells
Gynecology
Physiology
Silica
Sol-gel process
Sol-gels
Cytology
Catalano, P.N.
Bourguignon, N.S.
Alvarez, G.S.
Libertun, C.
Diaz, L.E.
Desimone, M.F.
Lux-Lantos, V.
Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion
topic_facet Adverse effect
Cell structure
Cell types
Cell viability
Cell-based
Delivery systems
In-vitro
Mammalian cells
Orthosilicate
Ovarian follicles
Proliferation rate
Silica matrix
Sol-gel silica
Steroid hormone
Tetraethoxysilanes
Tetrakis
Turner syndrome
Cells
Gynecology
Physiology
Silica
Sol-gel process
Sol-gels
Cytology
description The immobilization of mammalian cells within semipermeable matrices which allow retention of viability and functionality has recently attracted research interest. In this study, rat mature ovarian follicles were encapsulated in sol-gel silica matrices for the evaluation of in vitro steroid hormones production. Tetraethoxysilane (TEOS) and tetrakis(2-hydroxyethyl)orthosilicate (THEOS) were evaluated for cell viability, progesterone and estradiol secretion and cell structure conservation. Significant differences were observed between TEOS and THEOS, which allowed encapsulation of follicles for 9 days. Although encapsulated follicles showed lower viability and proliferation rates than control follicles, they were able to secrete estradiol to the same extent as control follicles. This study demonstrated an effective collaboration between two different mammalian cell types in hormone production and secretion when encapsulated in silica matrices. These results could lead to the development of ovarian cell-based hormone delivery systems for the treatment of the symptoms of menopause and the Turner syndrome minimizing adverse effects. © 2012 The Royal Society of Chemistry.
format JOUR
author Catalano, P.N.
Bourguignon, N.S.
Alvarez, G.S.
Libertun, C.
Diaz, L.E.
Desimone, M.F.
Lux-Lantos, V.
author_facet Catalano, P.N.
Bourguignon, N.S.
Alvarez, G.S.
Libertun, C.
Diaz, L.E.
Desimone, M.F.
Lux-Lantos, V.
author_sort Catalano, P.N.
title Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion
title_short Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion
title_full Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion
title_fullStr Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion
title_full_unstemmed Sol-gel immobilized ovarian follicles: Collaboration between two different cell types in hormone production and secretion
title_sort sol-gel immobilized ovarian follicles: collaboration between two different cell types in hormone production and secretion
url http://hdl.handle.net/20.500.12110/paper_09599428_v22_n23_p11681_Catalano
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AT alvarezgs solgelimmobilizedovarianfolliclescollaborationbetweentwodifferentcelltypesinhormoneproductionandsecretion
AT libertunc solgelimmobilizedovarianfolliclescollaborationbetweentwodifferentcelltypesinhormoneproductionandsecretion
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AT desimonemf solgelimmobilizedovarianfolliclescollaborationbetweentwodifferentcelltypesinhormoneproductionandsecretion
AT luxlantosv solgelimmobilizedovarianfolliclescollaborationbetweentwodifferentcelltypesinhormoneproductionandsecretion
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