Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol

Oxidants play a role in several stages of carcinogenesis. A high antioxidant capacity is expected to protect 'initiated' cells from excessive oxidant toxicity. The aim of this study was to determine the chemopreventive effect of α-tocopherol (α-T) on the hepatocarcinogenesis induced with p...

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Autores principales:	Gerez, E., Caballero, F., Vazquez, E., Polo, C., Del C. Batlle, A.M.
Formato:	JOUR
Materias:	α-Tocopherol Drug metabolizing enzyme system Heme metabolism Hepatocarcinogenesis Oxidative stress p-dimethylaminoazobenzene 4 dimethylaminoazobenzene 5 aminolevulinate synthase alpha tocopherol antioxidant cytochrome p450 glutathione transferase heme animal experiment animal model animal tissue antioxidant activity article controlled study liver cancer liver carcinogenesis liver metabolism male mouse nonhuman oxidative stress priority journal vitamin metabolism xenobiotic metabolism 5-Aminolevulinate Synthetase Animals Antioxidants Carcinogens Enzyme Induction Glutathione Transferase Liver Neoplasms, Experimental Male Mice Oxidative Stress p-Dimethylaminoazobenzene Vitamin E
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09598278_v7_n1_p69_Gerez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09598278_v7_n1_p69_Gerez
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spelling	todo:paper_09598278_v7_n1_p69_Gerez2023-10-03T15:53:14Z Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol Gerez, E. Caballero, F. Vazquez, E. Polo, C. Del C. Batlle, A.M. α-Tocopherol Drug metabolizing enzyme system Heme metabolism Hepatocarcinogenesis Oxidative stress p-dimethylaminoazobenzene 4 dimethylaminoazobenzene 5 aminolevulinate synthase alpha tocopherol antioxidant cytochrome p450 glutathione transferase heme animal experiment animal model animal tissue antioxidant activity article controlled study liver cancer liver carcinogenesis liver metabolism male mouse nonhuman oxidative stress priority journal vitamin metabolism xenobiotic metabolism 5-Aminolevulinate Synthetase Animals Antioxidants Carcinogens Enzyme Induction Glutathione Transferase Liver Neoplasms, Experimental Male Mice Oxidative Stress p-Dimethylaminoazobenzene Vitamin E Oxidants play a role in several stages of carcinogenesis. A high antioxidant capacity is expected to protect 'initiated' cells from excessive oxidant toxicity. The aim of this study was to determine the chemopreventive effect of α-tocopherol (α-T) on the hepatocarcinogenesis induced with p- dimethylaminoazobenzene (DAB) in mice. The dietary administration of α-T completely reversed the induction of δ-aminolevulinate synthetase and glutathione-S-transferase (the tumoral marker enzyme). α-T greatly enhanced P 450 levels, which were even higher in animals exposed to DAB. Indirect evidence for the involvement of oxygen radicals in the DAB model of hepatocarcinogenesis was provided by increased levels of thiobarbituric acid reactive species, which were detected in animals with severe liver damage and were assessed by histological analysis. α-T reduced the degree of hepatic injury, although this vitamin produced only slight changes in the oxidative parameters evaluated. The use of α-T as a potential chemopreventive agent, particularly during the initiation stage of carcinogenesis provoked by DAB, is worthy of further study. Fil:Gerez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09598278_v7_n1_p69_Gerez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	α-Tocopherol Drug metabolizing enzyme system Heme metabolism Hepatocarcinogenesis Oxidative stress p-dimethylaminoazobenzene 4 dimethylaminoazobenzene 5 aminolevulinate synthase alpha tocopherol antioxidant cytochrome p450 glutathione transferase heme animal experiment animal model animal tissue antioxidant activity article controlled study liver cancer liver carcinogenesis liver metabolism male mouse nonhuman oxidative stress priority journal vitamin metabolism xenobiotic metabolism 5-Aminolevulinate Synthetase Animals Antioxidants Carcinogens Enzyme Induction Glutathione Transferase Liver Neoplasms, Experimental Male Mice Oxidative Stress p-Dimethylaminoazobenzene Vitamin E
spellingShingle	α-Tocopherol Drug metabolizing enzyme system Heme metabolism Hepatocarcinogenesis Oxidative stress p-dimethylaminoazobenzene 4 dimethylaminoazobenzene 5 aminolevulinate synthase alpha tocopherol antioxidant cytochrome p450 glutathione transferase heme animal experiment animal model animal tissue antioxidant activity article controlled study liver cancer liver carcinogenesis liver metabolism male mouse nonhuman oxidative stress priority journal vitamin metabolism xenobiotic metabolism 5-Aminolevulinate Synthetase Animals Antioxidants Carcinogens Enzyme Induction Glutathione Transferase Liver Neoplasms, Experimental Male Mice Oxidative Stress p-Dimethylaminoazobenzene Vitamin E Gerez, E. Caballero, F. Vazquez, E. Polo, C. Del C. Batlle, A.M. Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol
topic_facet	α-Tocopherol Drug metabolizing enzyme system Heme metabolism Hepatocarcinogenesis Oxidative stress p-dimethylaminoazobenzene 4 dimethylaminoazobenzene 5 aminolevulinate synthase alpha tocopherol antioxidant cytochrome p450 glutathione transferase heme animal experiment animal model animal tissue antioxidant activity article controlled study liver cancer liver carcinogenesis liver metabolism male mouse nonhuman oxidative stress priority journal vitamin metabolism xenobiotic metabolism 5-Aminolevulinate Synthetase Animals Antioxidants Carcinogens Enzyme Induction Glutathione Transferase Liver Neoplasms, Experimental Male Mice Oxidative Stress p-Dimethylaminoazobenzene Vitamin E
description	Oxidants play a role in several stages of carcinogenesis. A high antioxidant capacity is expected to protect 'initiated' cells from excessive oxidant toxicity. The aim of this study was to determine the chemopreventive effect of α-tocopherol (α-T) on the hepatocarcinogenesis induced with p- dimethylaminoazobenzene (DAB) in mice. The dietary administration of α-T completely reversed the induction of δ-aminolevulinate synthetase and glutathione-S-transferase (the tumoral marker enzyme). α-T greatly enhanced P 450 levels, which were even higher in animals exposed to DAB. Indirect evidence for the involvement of oxygen radicals in the DAB model of hepatocarcinogenesis was provided by increased levels of thiobarbituric acid reactive species, which were detected in animals with severe liver damage and were assessed by histological analysis. α-T reduced the degree of hepatic injury, although this vitamin produced only slight changes in the oxidative parameters evaluated. The use of α-T as a potential chemopreventive agent, particularly during the initiation stage of carcinogenesis provoked by DAB, is worthy of further study.
format	JOUR
author	Gerez, E. Caballero, F. Vazquez, E. Polo, C. Del C. Batlle, A.M.
author_facet	Gerez, E. Caballero, F. Vazquez, E. Polo, C. Del C. Batlle, A.M.
author_sort	Gerez, E.
title	Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol
title_short	Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol
title_full	Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol
title_fullStr	Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol
title_full_unstemmed	Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol
title_sort	hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: protective role of α-tocopherol
url	http://hdl.handle.net/20.500.12110/paper_09598278_v7_n1_p69_Gerez
work_keys_str_mv	AT gereze hepaticenzymaticmetabolismalterationsandoxidativestressduringtheonsetofcarcinogenesisprotectiveroleofatocopherol AT caballerof hepaticenzymaticmetabolismalterationsandoxidativestressduringtheonsetofcarcinogenesisprotectiveroleofatocopherol AT vazqueze hepaticenzymaticmetabolismalterationsandoxidativestressduringtheonsetofcarcinogenesisprotectiveroleofatocopherol AT poloc hepaticenzymaticmetabolismalterationsandoxidativestressduringtheonsetofcarcinogenesisprotectiveroleofatocopherol AT delcbatlleam hepaticenzymaticmetabolismalterationsandoxidativestressduringtheonsetofcarcinogenesisprotectiveroleofatocopherol
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Hepatic enzymatic metabolism alterations and oxidative stress during the onset of carcinogenesis: Protective role of α-tocopherol

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