Altered protein mannosylation in developing cerebral cortex by streptomycin
Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affe...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09594965_v11_n16_p3569_Alperin |
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todo:paper_09594965_v11_n16_p3569_Alperin2023-10-03T15:52:58Z Altered protein mannosylation in developing cerebral cortex by streptomycin Alperin, M.D. Calandria, J.M. Carminatti, H. Idoyaga-Vargas, V.P. Cerebral cortex Mini-tissue units Neurotoxicity Protein glycosylation Streptomycin aminoglycoside antibiotic agent antibiotic agent asparagine linked oligosaccharide carbon 14 DNA glucose mannose streptomycin tritium animal tissue article brain cortex brain development concentration response controlled study DNA synthesis nervous system development neurotoxicity newborn nonhuman priority journal protein glycosylation protein synthesis rat Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affecting polypeptide synthesis, DNA synthesis or glucose and mannose disappearance from the medium in mini-tissue units derived from P5. In addition, the rate of Dolp-GlcNAc2Man9Glc3 synthesis and the oligosaccharide protein transferase activity did not change in the presence of the aminoglycoside. These findings strongly suggested that the alteration of protein mannosylation occurred downstream of G3 transfer to nascent polypeptides. Further, the mini-tissue units may be useful for the assessment of neurological toxicity of antibacterial agents. (C) 2000 Lippincott Williams and Wilkins. Fil:Alperin, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carminatti, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09594965_v11_n16_p3569_Alperin |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cerebral cortex Mini-tissue units Neurotoxicity Protein glycosylation Streptomycin aminoglycoside antibiotic agent antibiotic agent asparagine linked oligosaccharide carbon 14 DNA glucose mannose streptomycin tritium animal tissue article brain cortex brain development concentration response controlled study DNA synthesis nervous system development neurotoxicity newborn nonhuman priority journal protein glycosylation protein synthesis rat |
| spellingShingle |
Cerebral cortex Mini-tissue units Neurotoxicity Protein glycosylation Streptomycin aminoglycoside antibiotic agent antibiotic agent asparagine linked oligosaccharide carbon 14 DNA glucose mannose streptomycin tritium animal tissue article brain cortex brain development concentration response controlled study DNA synthesis nervous system development neurotoxicity newborn nonhuman priority journal protein glycosylation protein synthesis rat Alperin, M.D. Calandria, J.M. Carminatti, H. Idoyaga-Vargas, V.P. Altered protein mannosylation in developing cerebral cortex by streptomycin |
| topic_facet |
Cerebral cortex Mini-tissue units Neurotoxicity Protein glycosylation Streptomycin aminoglycoside antibiotic agent antibiotic agent asparagine linked oligosaccharide carbon 14 DNA glucose mannose streptomycin tritium animal tissue article brain cortex brain development concentration response controlled study DNA synthesis nervous system development neurotoxicity newborn nonhuman priority journal protein glycosylation protein synthesis rat |
| description |
Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affecting polypeptide synthesis, DNA synthesis or glucose and mannose disappearance from the medium in mini-tissue units derived from P5. In addition, the rate of Dolp-GlcNAc2Man9Glc3 synthesis and the oligosaccharide protein transferase activity did not change in the presence of the aminoglycoside. These findings strongly suggested that the alteration of protein mannosylation occurred downstream of G3 transfer to nascent polypeptides. Further, the mini-tissue units may be useful for the assessment of neurological toxicity of antibacterial agents. (C) 2000 Lippincott Williams and Wilkins. |
| format |
JOUR |
| author |
Alperin, M.D. Calandria, J.M. Carminatti, H. Idoyaga-Vargas, V.P. |
| author_facet |
Alperin, M.D. Calandria, J.M. Carminatti, H. Idoyaga-Vargas, V.P. |
| author_sort |
Alperin, M.D. |
| title |
Altered protein mannosylation in developing cerebral cortex by streptomycin |
| title_short |
Altered protein mannosylation in developing cerebral cortex by streptomycin |
| title_full |
Altered protein mannosylation in developing cerebral cortex by streptomycin |
| title_fullStr |
Altered protein mannosylation in developing cerebral cortex by streptomycin |
| title_full_unstemmed |
Altered protein mannosylation in developing cerebral cortex by streptomycin |
| title_sort |
altered protein mannosylation in developing cerebral cortex by streptomycin |
| url |
http://hdl.handle.net/20.500.12110/paper_09594965_v11_n16_p3569_Alperin |
| work_keys_str_mv |
AT alperinmd alteredproteinmannosylationindevelopingcerebralcortexbystreptomycin AT calandriajm alteredproteinmannosylationindevelopingcerebralcortexbystreptomycin AT carminattih alteredproteinmannosylationindevelopingcerebralcortexbystreptomycin AT idoyagavargasvp alteredproteinmannosylationindevelopingcerebralcortexbystreptomycin |
| _version_ |
1807317141733507072 |