Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation

Six Rhizobium meliloti mutants were isolated after Tn5-mediated mutagenesis as resistant to inhibition by a mixture of amino acids (serine, methionine, glycine and leucine). All were defective in adenylate cyclase activity and failed to form nodules in infected roots of Medicago sativa. Furthermore,...

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Autores principales: Bianchini, G.M., Carricarte, V.C., Flawia, M.M., Sanchez-Rivas, C.
Formato: JOUR
Materias:
Adenylate cyclase
amino acid sensitivity
exopolysaccharide
nodulation
Rhizobium meliloti
Tn5 mutagenesis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09593993_v9_n2_p168_Bianchini
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_09593993_v9_n2_p168_Bianchini2023-10-03T15:52:55Z Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation Bianchini, G.M. Carricarte, V.C. Flawia, M.M. Sanchez-Rivas, C. Adenylate cyclase amino acid sensitivity exopolysaccharide nodulation Rhizobium meliloti Tn5 mutagenesis Six Rhizobium meliloti mutants were isolated after Tn5-mediated mutagenesis as resistant to inhibition by a mixture of amino acids (serine, methionine, glycine and leucine). All were defective in adenylate cyclase activity and failed to form nodules in infected roots of Medicago sativa. Furthermore, like other nodulation mutants, they showed altered motility and increased secretion of exopolysaccharides; addition of cAMP to the growth medium abolished some of these phenotypic defects. The possibility that adenylate cyclase participates in the transduction of signals inducing nodulation is discussed. © 1993 Rapid Communications of Oxford Ltd. Fil:Bianchini, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carricarte, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09593993_v9_n2_p168_Bianchini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adenylate cyclase
amino acid sensitivity
exopolysaccharide
nodulation
Rhizobium meliloti
Tn5 mutagenesis
spellingShingle Adenylate cyclase
amino acid sensitivity
exopolysaccharide
nodulation
Rhizobium meliloti
Tn5 mutagenesis
Bianchini, G.M.
Carricarte, V.C.
Flawia, M.M.
Sanchez-Rivas, C.
Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
topic_facet Adenylate cyclase
amino acid sensitivity
exopolysaccharide
nodulation
Rhizobium meliloti
Tn5 mutagenesis
description Six Rhizobium meliloti mutants were isolated after Tn5-mediated mutagenesis as resistant to inhibition by a mixture of amino acids (serine, methionine, glycine and leucine). All were defective in adenylate cyclase activity and failed to form nodules in infected roots of Medicago sativa. Furthermore, like other nodulation mutants, they showed altered motility and increased secretion of exopolysaccharides; addition of cAMP to the growth medium abolished some of these phenotypic defects. The possibility that adenylate cyclase participates in the transduction of signals inducing nodulation is discussed. © 1993 Rapid Communications of Oxford Ltd.
format JOUR
author Bianchini, G.M.
Carricarte, V.C.
Flawia, M.M.
Sanchez-Rivas, C.
author_facet Bianchini, G.M.
Carricarte, V.C.
Flawia, M.M.
Sanchez-Rivas, C.
author_sort Bianchini, G.M.
title Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_short Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_full Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_fullStr Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_full_unstemmed Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_sort isolation of adenylate cyclase mutants from rhizobium meliloti deficient in nodulation
url http://hdl.handle.net/20.500.12110/paper_09593993_v9_n2_p168_Bianchini
work_keys_str_mv AT bianchinigm isolationofadenylatecyclasemutantsfromrhizobiummelilotideficientinnodulation
AT carricartevc isolationofadenylatecyclasemutantsfromrhizobiummelilotideficientinnodulation
AT flawiamm isolationofadenylatecyclasemutantsfromrhizobiummelilotideficientinnodulation
AT sanchezrivasc isolationofadenylatecyclasemutantsfromrhizobiummelilotideficientinnodulation
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