The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro

In the present study, we obtained in vitro dual cultures between the liverwort Plagiochasma rupestre and two arbuscular mycorrhizal (AM) fungi: Glomus intraradices and Glomus clarum. Four agarized culture media were tested for optimal growth of P. rupestre. Also, a description of the symbiotic assoc...

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Autores principales:	Silvani, V.A., Rothen, C.P., Rodríguez, M.A., Godeas, A., Fracchia, S.
Formato:	JOUR
Materias:	Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus intraradices Growth conditions In-vitro Optimal growth Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Glomus clarum Marchantiophyta
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09593993_v28_n12_p3393_Silvani
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09593993_v28_n12_p3393_Silvani
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spelling	todo:paper_09593993_v28_n12_p3393_Silvani2023-10-03T15:52:52Z The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro Silvani, V.A. Rothen, C.P. Rodríguez, M.A. Godeas, A. Fracchia, S. Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre In the present study, we obtained in vitro dual cultures between the liverwort Plagiochasma rupestre and two arbuscular mycorrhizal (AM) fungi: Glomus intraradices and Glomus clarum. Four agarized culture media were tested for optimal growth of P. rupestre. Also, a description of the symbiotic association is provided. Plagiochasma rupestre gametophytes profusely grew axenically in MM with sucrose, and thalli were successfully subcultured under these growth conditions. Arbuscular mycorrhizal fungal hyphae colonized P. rupestre thalli through rhizoids or by forming appresoria in the ventral thallus cells. Arbuscules, mycelia and structures resembling intrathallic spores or vesicles were developed in the internal parenchymatic cells. The pattern of AM colonization in P. rupestre was very similar to the Paris-type. After 100 days of dual culture, the external mycelia of both AM fungal strains formed thousands of small viable spores, suggesting that P. rupestre in vitro culture could be a valuable tool for studying the biology of both symbiotic partners and conserving AM fungi in in vitro germplasm collections. © 2012 Springer Science+Business Media B.V. Fil:Silvani, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fracchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09593993_v28_n12_p3393_Silvani
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre
spellingShingle	Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre Silvani, V.A. Rothen, C.P. Rodríguez, M.A. Godeas, A. Fracchia, S. The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
topic_facet	Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre
description	In the present study, we obtained in vitro dual cultures between the liverwort Plagiochasma rupestre and two arbuscular mycorrhizal (AM) fungi: Glomus intraradices and Glomus clarum. Four agarized culture media were tested for optimal growth of P. rupestre. Also, a description of the symbiotic association is provided. Plagiochasma rupestre gametophytes profusely grew axenically in MM with sucrose, and thalli were successfully subcultured under these growth conditions. Arbuscular mycorrhizal fungal hyphae colonized P. rupestre thalli through rhizoids or by forming appresoria in the ventral thallus cells. Arbuscules, mycelia and structures resembling intrathallic spores or vesicles were developed in the internal parenchymatic cells. The pattern of AM colonization in P. rupestre was very similar to the Paris-type. After 100 days of dual culture, the external mycelia of both AM fungal strains formed thousands of small viable spores, suggesting that P. rupestre in vitro culture could be a valuable tool for studying the biology of both symbiotic partners and conserving AM fungi in in vitro germplasm collections. © 2012 Springer Science+Business Media B.V.
format	JOUR
author	Silvani, V.A. Rothen, C.P. Rodríguez, M.A. Godeas, A. Fracchia, S.
author_facet	Silvani, V.A. Rothen, C.P. Rodríguez, M.A. Godeas, A. Fracchia, S.
author_sort	Silvani, V.A.
title	The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
title_short	The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
title_full	The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
title_fullStr	The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
title_full_unstemmed	The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
title_sort	thalloid liverwort plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
url	http://hdl.handle.net/20.500.12110/paper_09593993_v28_n12_p3393_Silvani
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The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro

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