Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)

Cyanobacteria produce a wide array of substances with activity in many biological systems. The aim of the present research was to compare the effect of differently treated extracellular products (EP) from Tolypothrix tenuis (Cyanobacteria) on rice plantlet regeneration as well as on the pigments, pr...

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Autores principales: De Cano, M.S., Zaccaro, M.C., García, I., Stella, A.M., De Caire, G.Z.
Formato: JOUR
Materias:
Bioactive extracellular substances
Cyanobacteria
Plant growth regulators
Plant regeneration
Porphyrin
Rice callus
Tolypothrix tenuis
Calothrix sp. PCC 7101
Tolypothrix
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09593993_v19_n1_p29_DeCano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09593993_v19_n1_p29_DeCano
record_format dspace
spelling todo:paper_09593993_v19_n1_p29_DeCano2023-10-03T15:52:50Z Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria) De Cano, M.S. Zaccaro, M.C. García, I. Stella, A.M. De Caire, G.Z. Bioactive extracellular substances Cyanobacteria Plant growth regulators Plant regeneration Porphyrin Rice callus Tolypothrix tenuis Calothrix sp. PCC 7101 Cyanobacteria Tolypothrix Cyanobacteria produce a wide array of substances with activity in many biological systems. The aim of the present research was to compare the effect of differently treated extracellular products (EP) from Tolypothrix tenuis (Cyanobacteria) on rice plantlet regeneration as well as on the pigments, protein, total free porphyrin contents, and 5-aminolevulinate dehydratase (ALA-D) activity in rice callus during differentiation. Rice embryo calli were regenerated in Murashige & Skoog medium supplemented with EP with protein (EPp) or without protein (EPnp) or autoclaved (EPa), as well as with benzyladenine (BA) and benzyladenine + naphthaleneacetic acid (BA + NAA). At day 75, calli percentage regeneration were: EPnp (84%), EPp (58%), BA + NAA (45%), BA (44%), EPa (40%). The same trend was found for chlorophyll a, b, total and carotenoid contents. Protein content in BA, BA + NAA and EPnp treatments was 35% higher than in EPp and EPa. Total free porphyrin was similar in all treatments. ALA-D activity in BA, EPp and EPa treatments was 28% higher than in BA + NAA and EPnp. The extracellular bioactive substance(s) from T. tenuis would contain a mixture of thermolabile plant growth regulators that replaced and improved the effects of synthetic plant growth regulators on rice callus organogenesis. Calli were rhizogenic in all the regeneration media tested. The pigment content of the calli was related to percentage regeneration but not to the total free porphyrin and ALA-D activity. Fil:Zaccaro, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09593993_v19_n1_p29_DeCano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bioactive extracellular substances
Cyanobacteria
Plant growth regulators
Plant regeneration
Porphyrin
Rice callus
Tolypothrix tenuis
Calothrix sp. PCC 7101
Cyanobacteria
Tolypothrix
spellingShingle Bioactive extracellular substances
Cyanobacteria
Plant growth regulators
Plant regeneration
Porphyrin
Rice callus
Tolypothrix tenuis
Calothrix sp. PCC 7101
Cyanobacteria
Tolypothrix
De Cano, M.S.
Zaccaro, M.C.
García, I.
Stella, A.M.
De Caire, G.Z.
Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
topic_facet Bioactive extracellular substances
Cyanobacteria
Plant growth regulators
Plant regeneration
Porphyrin
Rice callus
Tolypothrix tenuis
Calothrix sp. PCC 7101
Cyanobacteria
Tolypothrix
description Cyanobacteria produce a wide array of substances with activity in many biological systems. The aim of the present research was to compare the effect of differently treated extracellular products (EP) from Tolypothrix tenuis (Cyanobacteria) on rice plantlet regeneration as well as on the pigments, protein, total free porphyrin contents, and 5-aminolevulinate dehydratase (ALA-D) activity in rice callus during differentiation. Rice embryo calli were regenerated in Murashige & Skoog medium supplemented with EP with protein (EPp) or without protein (EPnp) or autoclaved (EPa), as well as with benzyladenine (BA) and benzyladenine + naphthaleneacetic acid (BA + NAA). At day 75, calli percentage regeneration were: EPnp (84%), EPp (58%), BA + NAA (45%), BA (44%), EPa (40%). The same trend was found for chlorophyll a, b, total and carotenoid contents. Protein content in BA, BA + NAA and EPnp treatments was 35% higher than in EPp and EPa. Total free porphyrin was similar in all treatments. ALA-D activity in BA, EPp and EPa treatments was 28% higher than in BA + NAA and EPnp. The extracellular bioactive substance(s) from T. tenuis would contain a mixture of thermolabile plant growth regulators that replaced and improved the effects of synthetic plant growth regulators on rice callus organogenesis. Calli were rhizogenic in all the regeneration media tested. The pigment content of the calli was related to percentage regeneration but not to the total free porphyrin and ALA-D activity.
format JOUR
author De Cano, M.S.
Zaccaro, M.C.
García, I.
Stella, A.M.
De Caire, G.Z.
author_facet De Cano, M.S.
Zaccaro, M.C.
García, I.
Stella, A.M.
De Caire, G.Z.
author_sort De Cano, M.S.
title Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_short Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_full Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_fullStr Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_full_unstemmed Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_sort enhancing rice callus regeneration by extracellular products of tolypothrix tenuis (cyanobacteria)
url http://hdl.handle.net/20.500.12110/paper_09593993_v19_n1_p29_DeCano
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AT garciai enhancingricecallusregenerationbyextracellularproductsoftolypothrixtenuiscyanobacteria
AT stellaam enhancingricecallusregenerationbyextracellularproductsoftolypothrixtenuiscyanobacteria
AT decairegz enhancingricecallusregenerationbyextracellularproductsoftolypothrixtenuiscyanobacteria
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