A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere

Selection and evaluation of microbial strains for their antifungal activity in natural environments is time- and energy-consuming. We have adapted a PCR-based method to avoid these inconveniences. Soils that are naturally suppressive to plant disease were chosen as a source of antibiotic-producing b...

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Detalles Bibliográficos
Autores principales: Giacomodonato, M.N., Pettinari, M.J., Souto, G.I., Méndez, B.S., López, N.I.
Formato: JOUR
Materias:
Antifungal activity
Bacillus sp
PCR
Peptide synthetase genes
Rhizosphere
Suppressive soybean soils
antifungal activity
biological pest control
culture medium
DNA template
fungal infection
genetic screening
peptide synthase
plant disease control
polymerase chain reaction
rhizosphere
signal transduction
soybean
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09593993_v17_n1_p51_Giacomodonato
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09593993_v17_n1_p51_Giacomodonato
record_format dspace
spelling todo:paper_09593993_v17_n1_p51_Giacomodonato2023-10-03T15:52:50Z A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere Giacomodonato, M.N. Pettinari, M.J. Souto, G.I. Méndez, B.S. López, N.I. Antifungal activity Bacillus sp PCR Peptide synthetase genes Rhizosphere Suppressive soybean soils antifungal activity biological pest control culture medium DNA template fungal infection genetic screening peptide synthase plant disease control polymerase chain reaction rhizosphere signal transduction soybean Selection and evaluation of microbial strains for their antifungal activity in natural environments is time- and energy-consuming. We have adapted a PCR-based method to avoid these inconveniences. Soils that are naturally suppressive to plant disease were chosen as a source of antibiotic-producing bacteria. The screening was performed by means of PCR amplification using degenerate primers corresponding to peptide synthetase genes. Amplification fragments were obtained using template DNA from the rhizosphere of three different soybean fields. In order to assay their potential utility in pathogen control, several Bacillus strains were analysed for their in vitro antifungal activity by testing growth inhibition of Sclerotinia sclerotiorum. Four Bacillus sp. isolates gave a positive amplification signal, and three of them had an inhibitory effect on S. sclerotiorum growth, whereas two strains that failed to give an amplification signal did not inhibit fungal growth. These results show that PCR-based techniques could be useful to assess the presence of strains with potential use as biocontrol agents. Fil:Giacomodonato, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Souto, G.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09593993_v17_n1_p51_Giacomodonato
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antifungal activity
Bacillus sp
PCR
Peptide synthetase genes
Rhizosphere
Suppressive soybean soils
antifungal activity
biological pest control
culture medium
DNA template
fungal infection
genetic screening
peptide synthase
plant disease control
polymerase chain reaction
rhizosphere
signal transduction
soybean
spellingShingle Antifungal activity
Bacillus sp
PCR
Peptide synthetase genes
Rhizosphere
Suppressive soybean soils
antifungal activity
biological pest control
culture medium
DNA template
fungal infection
genetic screening
peptide synthase
plant disease control
polymerase chain reaction
rhizosphere
signal transduction
soybean
Giacomodonato, M.N.
Pettinari, M.J.
Souto, G.I.
Méndez, B.S.
López, N.I.
A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere
topic_facet Antifungal activity
Bacillus sp
PCR
Peptide synthetase genes
Rhizosphere
Suppressive soybean soils
antifungal activity
biological pest control
culture medium
DNA template
fungal infection
genetic screening
peptide synthase
plant disease control
polymerase chain reaction
rhizosphere
signal transduction
soybean
description Selection and evaluation of microbial strains for their antifungal activity in natural environments is time- and energy-consuming. We have adapted a PCR-based method to avoid these inconveniences. Soils that are naturally suppressive to plant disease were chosen as a source of antibiotic-producing bacteria. The screening was performed by means of PCR amplification using degenerate primers corresponding to peptide synthetase genes. Amplification fragments were obtained using template DNA from the rhizosphere of three different soybean fields. In order to assay their potential utility in pathogen control, several Bacillus strains were analysed for their in vitro antifungal activity by testing growth inhibition of Sclerotinia sclerotiorum. Four Bacillus sp. isolates gave a positive amplification signal, and three of them had an inhibitory effect on S. sclerotiorum growth, whereas two strains that failed to give an amplification signal did not inhibit fungal growth. These results show that PCR-based techniques could be useful to assess the presence of strains with potential use as biocontrol agents.
format JOUR
author Giacomodonato, M.N.
Pettinari, M.J.
Souto, G.I.
Méndez, B.S.
López, N.I.
author_facet Giacomodonato, M.N.
Pettinari, M.J.
Souto, G.I.
Méndez, B.S.
López, N.I.
author_sort Giacomodonato, M.N.
title A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere
title_short A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere
title_full A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere
title_fullStr A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere
title_full_unstemmed A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere
title_sort pcr-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere
url http://hdl.handle.net/20.500.12110/paper_09593993_v17_n1_p51_Giacomodonato
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