High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography
An automated micellar electrokinetic capillary chromatographic method has been developed in order to determine xanthines, e.g. caffeine, theobromine and theophylline, and chlorogenic acid in samples of yerba mate (Ilex paraguariensis). The target constituents were detected by photodiode array, and q...
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todo:paper_09580344_v13_n4_p235_Pomilio2023-10-03T15:52:36Z High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography Pomilio, A.B. Trajtemberg, S. Vitale, A.A. Electron-impact mass spectrometry High-performance capillary electrophoresis Ilex paraguariensis Mate infusions Micellar electrokinetic capillary chromatography Xanthines caffeine chlorogenic acid theobromine theophylline xanthine derivative analytic method article capillary electrophoresis consumer controlled study gastrointestinal disease ilex micellar electrokinetic chromatography nonhuman phytochemistry plant plant leaf plant stem powder quantitative analysis temperature automation chemistry electrospray mass spectrometry Ilex paraguariensis methodology plant stem Automation Chromatography, Micellar Electrokinetic Capillary Ilex paraguariensis Plant Leaves Plant Stems Spectrometry, Mass, Electrospray Ionization Support, Non-U.S. Gov't Automation Plant Leaves Spectrometry, Mass, Electrospray Ionization Croton ovalifolius Ilex Ilex paraguariensis An automated micellar electrokinetic capillary chromatographic method has been developed in order to determine xanthines, e.g. caffeine, theobromine and theophylline, and chlorogenic acid in samples of yerba mate (Ilex paraguariensis). The target constituents were detected by photodiode array, and quantified by an external standard method. In addition, each constituent was collected separately and identified by EIMS. The method has been used to analyse 30 samples of mate infusions prepared at 30 and 75°C with milled leaves and stems of 14 commercial brands which had been subjected to different elaboration process. Suspended powdered material of each infusion was also analysed after three sieving steps. There was a remarkable difference in the relative xanthine composition of the finely suspended material, the amount of which varied according to the yerba mate brand, the elaboration process and the temperature of the infusion. The importance of these results with respect to gastrointestinal disorders which have been observed by habitual consumers of mate are discussed. Copyright © 2002 John Wiley & Sons, Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09580344_v13_n4_p235_Pomilio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Electron-impact mass spectrometry High-performance capillary electrophoresis Ilex paraguariensis Mate infusions Micellar electrokinetic capillary chromatography Xanthines caffeine chlorogenic acid theobromine theophylline xanthine derivative analytic method article capillary electrophoresis consumer controlled study gastrointestinal disease ilex micellar electrokinetic chromatography nonhuman phytochemistry plant plant leaf plant stem powder quantitative analysis temperature automation chemistry electrospray mass spectrometry Ilex paraguariensis methodology plant stem Automation Chromatography, Micellar Electrokinetic Capillary Ilex paraguariensis Plant Leaves Plant Stems Spectrometry, Mass, Electrospray Ionization Support, Non-U.S. Gov't Automation Plant Leaves Spectrometry, Mass, Electrospray Ionization Croton ovalifolius Ilex Ilex paraguariensis |
| spellingShingle |
Electron-impact mass spectrometry High-performance capillary electrophoresis Ilex paraguariensis Mate infusions Micellar electrokinetic capillary chromatography Xanthines caffeine chlorogenic acid theobromine theophylline xanthine derivative analytic method article capillary electrophoresis consumer controlled study gastrointestinal disease ilex micellar electrokinetic chromatography nonhuman phytochemistry plant plant leaf plant stem powder quantitative analysis temperature automation chemistry electrospray mass spectrometry Ilex paraguariensis methodology plant stem Automation Chromatography, Micellar Electrokinetic Capillary Ilex paraguariensis Plant Leaves Plant Stems Spectrometry, Mass, Electrospray Ionization Support, Non-U.S. Gov't Automation Plant Leaves Spectrometry, Mass, Electrospray Ionization Croton ovalifolius Ilex Ilex paraguariensis Pomilio, A.B. Trajtemberg, S. Vitale, A.A. High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography |
| topic_facet |
Electron-impact mass spectrometry High-performance capillary electrophoresis Ilex paraguariensis Mate infusions Micellar electrokinetic capillary chromatography Xanthines caffeine chlorogenic acid theobromine theophylline xanthine derivative analytic method article capillary electrophoresis consumer controlled study gastrointestinal disease ilex micellar electrokinetic chromatography nonhuman phytochemistry plant plant leaf plant stem powder quantitative analysis temperature automation chemistry electrospray mass spectrometry Ilex paraguariensis methodology plant stem Automation Chromatography, Micellar Electrokinetic Capillary Ilex paraguariensis Plant Leaves Plant Stems Spectrometry, Mass, Electrospray Ionization Support, Non-U.S. Gov't Automation Plant Leaves Spectrometry, Mass, Electrospray Ionization Croton ovalifolius Ilex Ilex paraguariensis |
| description |
An automated micellar electrokinetic capillary chromatographic method has been developed in order to determine xanthines, e.g. caffeine, theobromine and theophylline, and chlorogenic acid in samples of yerba mate (Ilex paraguariensis). The target constituents were detected by photodiode array, and quantified by an external standard method. In addition, each constituent was collected separately and identified by EIMS. The method has been used to analyse 30 samples of mate infusions prepared at 30 and 75°C with milled leaves and stems of 14 commercial brands which had been subjected to different elaboration process. Suspended powdered material of each infusion was also analysed after three sieving steps. There was a remarkable difference in the relative xanthine composition of the finely suspended material, the amount of which varied according to the yerba mate brand, the elaboration process and the temperature of the infusion. The importance of these results with respect to gastrointestinal disorders which have been observed by habitual consumers of mate are discussed. Copyright © 2002 John Wiley & Sons, Ltd. |
| format |
JOUR |
| author |
Pomilio, A.B. Trajtemberg, S. Vitale, A.A. |
| author_facet |
Pomilio, A.B. Trajtemberg, S. Vitale, A.A. |
| author_sort |
Pomilio, A.B. |
| title |
High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography |
| title_short |
High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography |
| title_full |
High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography |
| title_fullStr |
High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography |
| title_full_unstemmed |
High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography |
| title_sort |
high-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of ilex paraguariensis using automated micellar electrokinetic capillary chromatography |
| url |
http://hdl.handle.net/20.500.12110/paper_09580344_v13_n4_p235_Pomilio |
| work_keys_str_mv |
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