Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarate from 2-oxoglutaric acid. An innovative combination strategy that uses pure...
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todo:paper_09574166_v15_n23_p3763_Rustoy2023-10-03T15:52:23Z Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid Rustoy, E.M. Pereyra, E.N. Moreno, S. Baldessari, A. 2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarate from 2-oxoglutaric acid. An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarates from 2-oxoglutaric acid. The method involves two consecutive biocatalytic steps. The first step, which converts the 2-oxoglutaric acid into the corresponding dialkyl esters, was catalyzed by a lipase. Then in the second step, by microbial reduction of the dialkyl-2-oxoglutarates, it is possible to obtain 3-carboxyalkyl-γ- butyrolactones or 2-hydroxyesters depending on the length of the chain in the alkyl moiety of the esters and on the fresh or lyophilized status of the cells. © 2004 Elsevier Ltd. All rights reserved. Fil:Rustoy, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereyra, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baldessari, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09574166_v15_n23_p3763_Rustoy |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal |
| spellingShingle |
2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal Rustoy, E.M. Pereyra, E.N. Moreno, S. Baldessari, A. Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid |
| topic_facet |
2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal |
| description |
An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarate from 2-oxoglutaric acid. An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarates from 2-oxoglutaric acid. The method involves two consecutive biocatalytic steps. The first step, which converts the 2-oxoglutaric acid into the corresponding dialkyl esters, was catalyzed by a lipase. Then in the second step, by microbial reduction of the dialkyl-2-oxoglutarates, it is possible to obtain 3-carboxyalkyl-γ- butyrolactones or 2-hydroxyesters depending on the length of the chain in the alkyl moiety of the esters and on the fresh or lyophilized status of the cells. © 2004 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Rustoy, E.M. Pereyra, E.N. Moreno, S. Baldessari, A. |
| author_facet |
Rustoy, E.M. Pereyra, E.N. Moreno, S. Baldessari, A. |
| author_sort |
Rustoy, E.M. |
| title |
Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid |
| title_short |
Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid |
| title_full |
Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid |
| title_fullStr |
Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid |
| title_full_unstemmed |
Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid |
| title_sort |
combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid |
| url |
http://hdl.handle.net/20.500.12110/paper_09574166_v15_n23_p3763_Rustoy |
| work_keys_str_mv |
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1807315598379581440 |