Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture

Toxigenic Aspergillus flavus and A. parasiticus fungal strains can contaminate a wide variety of food crops with the subsequent production of aflatoxins (AFs) resulting in severe economic losses and public health issues. Biological control is a promising approach to manage AFs contamination in pre-...

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Autores principales:	González Pereyra, M.L., Martínez, M.P., Petroselli, G., Erra Balsells, R., Cavaglieri, L.R.
Formato:	JOUR
Materias:	Aflatoxin B1 Aspergillus parasiticus Bacillus Lipopeptides
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09567135_v85_n_p392_GonzalezPereyra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09567135_v85_n_p392_GonzalezPereyra
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spelling	todo:paper_09567135_v85_n_p392_GonzalezPereyra2023-10-03T15:52:16Z Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture González Pereyra, M.L. Martínez, M.P. Petroselli, G. Erra Balsells, R. Cavaglieri, L.R. Aflatoxin B1 Aspergillus parasiticus Bacillus Lipopeptides Toxigenic Aspergillus flavus and A. parasiticus fungal strains can contaminate a wide variety of food crops with the subsequent production of aflatoxins (AFs) resulting in severe economic losses and public health issues. Biological control is a promising approach to manage AFs contamination in pre- and post-harvested crops. In the present study, the effect of soil-borne Bacillus spp. strains on aflatoxigenic A. parasiticus growth and AFs production was evaluated and the culture supernatant of the most effective strain was evaluated for the presence of antifungal lipopeptides. Six Bacillus spp. strains were able to reduce A. parasiticus growth rate significantly (p < 0.05). Bacillus spp. RC1A was able to inhibit fungal growth almost completely, reducing growth rate to 0.16 mm/h and increasing Lag phase duration (31.72 h) (p < 0.0001). RC1A could also reduce AFB1 concentration produced by A. parasiticus (p < 0.0001). Organic solvent extraction and chromatographic analysis of RC1A culture supernatant showed the presence of bands corresponding to three of the main groups of lipopeptides (surfactin, iturin A and fengycin) at the expected retention factor (Rf) values; they were also confirmed by MALDI-MS analysis. These fractions were able to inhibit A. parasiticus growth and AFB1 production to non-detectable levels when tested separately in liquid culture media. The further study of the antifungal compounds produced by these strains will determine their potential use to manage AFs contamination in crops and feeds. © 2017 Elsevier Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09567135_v85_n_p392_GonzalezPereyra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Aflatoxin B1 Aspergillus parasiticus Bacillus Lipopeptides
spellingShingle	Aflatoxin B1 Aspergillus parasiticus Bacillus Lipopeptides González Pereyra, M.L. Martínez, M.P. Petroselli, G. Erra Balsells, R. Cavaglieri, L.R. Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
topic_facet	Aflatoxin B1 Aspergillus parasiticus Bacillus Lipopeptides
description	Toxigenic Aspergillus flavus and A. parasiticus fungal strains can contaminate a wide variety of food crops with the subsequent production of aflatoxins (AFs) resulting in severe economic losses and public health issues. Biological control is a promising approach to manage AFs contamination in pre- and post-harvested crops. In the present study, the effect of soil-borne Bacillus spp. strains on aflatoxigenic A. parasiticus growth and AFs production was evaluated and the culture supernatant of the most effective strain was evaluated for the presence of antifungal lipopeptides. Six Bacillus spp. strains were able to reduce A. parasiticus growth rate significantly (p < 0.05). Bacillus spp. RC1A was able to inhibit fungal growth almost completely, reducing growth rate to 0.16 mm/h and increasing Lag phase duration (31.72 h) (p < 0.0001). RC1A could also reduce AFB1 concentration produced by A. parasiticus (p < 0.0001). Organic solvent extraction and chromatographic analysis of RC1A culture supernatant showed the presence of bands corresponding to three of the main groups of lipopeptides (surfactin, iturin A and fengycin) at the expected retention factor (Rf) values; they were also confirmed by MALDI-MS analysis. These fractions were able to inhibit A. parasiticus growth and AFB1 production to non-detectable levels when tested separately in liquid culture media. The further study of the antifungal compounds produced by these strains will determine their potential use to manage AFs contamination in crops and feeds. © 2017 Elsevier Ltd
format	JOUR
author	González Pereyra, M.L. Martínez, M.P. Petroselli, G. Erra Balsells, R. Cavaglieri, L.R.
author_facet	González Pereyra, M.L. Martínez, M.P. Petroselli, G. Erra Balsells, R. Cavaglieri, L.R.
author_sort	González Pereyra, M.L.
title	Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_short	Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_full	Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_fullStr	Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_full_unstemmed	Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_sort	antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil bacillus strains with potential application in agriculture
url	http://hdl.handle.net/20.500.12110/paper_09567135_v85_n_p392_GonzalezPereyra
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Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture

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