Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease
The high sensitivity that can be attained using an immunoassays coupled to a rotating bioreactor with electrochemical detection mediated by [Os(bpy)2Cl(pyCOOH)]Cl, has been verified for the detection of Trypanozoma cruzi (T. cruzi), This protozoan parasite causes Chagas disease, affecting more than...
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todo:paper_09565663_v21_n2_p313_Salinas2023-10-03T15:52:08Z Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease Salinas, E. Torriero, A.A.J. Battaglini, F. Sanz, M.I. Olsina, R. Raba, J. Biosensors Flow system Horseradish peroxidase Immunoassays Osmium Trypanosoma cruzi Antibodies Bioassay Complexation Diseases Enzymes Hydrogen peroxide Immunology Osmium compounds Protozoa Spectrophotometry Chagas disease Horseradisg peroxidase (HRP) enzymes Immunoassays Serum Bioreactors horseradish peroxidase human immunoglobulin hydrogen peroxide immobilized antibody immunoglobulin G parasite antibody parasite antigen amperometry analytical error antibody labeling antigen antibody reaction article bioreactor bioreactor design Chagas disease continuous flow reactor enzyme immunoassay parasite identification quantitative analysis reproducibility spectrophotometry Trypanosoma cruzi Animals Antibodies, Protozoan Bioreactors Chagas Disease Equipment Design Equipment Failure Analysis Flow Injection Analysis Humans Immunoenzyme Techniques Rotation Trypanosoma cruzi Armoracia rusticana Protozoa Trypanosoma cruzi The high sensitivity that can be attained using an immunoassays coupled to a rotating bioreactor with electrochemical detection mediated by [Os(bpy)2Cl(pyCOOH)]Cl, has been verified for the detection of Trypanozoma cruzi (T. cruzi), This protozoan parasite causes Chagas disease, affecting more than 18 million people in central and south America. Antibodies in the serum sample are allowed to react immunologically with whole homogenates of the parasite as antigen that are immobilized on a rotating disk. The bound antibodies are quantified by a horseradish peroxidase (HRP) enzyme labeled second antibodies specific to human IgG in presence of hydrogen peroxide using an osmium complex [Os(bpy)2Cl(pyCOOH)]Cl as enzymatic mediators. The amperometric measurement performed at 0.00 V versus Ag/AgCl can be done within 2 min and the analysis time does not exceed 23 min. The calculated detection limits was 0.01 mIU ml-1. Reproducibility assays were made using repetitive serum of 0.182 mIU ml-1 T. cruzi specific antibody (measured as the activity of the correspondent anti-serum's enzyme conjugated); the percentage standard error was less than 5%. The amperometric immunoreactors showed significantly higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method. © 2004 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09565663_v21_n2_p313_Salinas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biosensors Flow system Horseradish peroxidase Immunoassays Osmium Trypanosoma cruzi Antibodies Bioassay Complexation Diseases Enzymes Hydrogen peroxide Immunology Osmium compounds Protozoa Spectrophotometry Chagas disease Horseradisg peroxidase (HRP) enzymes Immunoassays Serum Bioreactors horseradish peroxidase human immunoglobulin hydrogen peroxide immobilized antibody immunoglobulin G parasite antibody parasite antigen amperometry analytical error antibody labeling antigen antibody reaction article bioreactor bioreactor design Chagas disease continuous flow reactor enzyme immunoassay parasite identification quantitative analysis reproducibility spectrophotometry Trypanosoma cruzi Animals Antibodies, Protozoan Bioreactors Chagas Disease Equipment Design Equipment Failure Analysis Flow Injection Analysis Humans Immunoenzyme Techniques Rotation Trypanosoma cruzi Armoracia rusticana Protozoa Trypanosoma cruzi |
| spellingShingle |
Biosensors Flow system Horseradish peroxidase Immunoassays Osmium Trypanosoma cruzi Antibodies Bioassay Complexation Diseases Enzymes Hydrogen peroxide Immunology Osmium compounds Protozoa Spectrophotometry Chagas disease Horseradisg peroxidase (HRP) enzymes Immunoassays Serum Bioreactors horseradish peroxidase human immunoglobulin hydrogen peroxide immobilized antibody immunoglobulin G parasite antibody parasite antigen amperometry analytical error antibody labeling antigen antibody reaction article bioreactor bioreactor design Chagas disease continuous flow reactor enzyme immunoassay parasite identification quantitative analysis reproducibility spectrophotometry Trypanosoma cruzi Animals Antibodies, Protozoan Bioreactors Chagas Disease Equipment Design Equipment Failure Analysis Flow Injection Analysis Humans Immunoenzyme Techniques Rotation Trypanosoma cruzi Armoracia rusticana Protozoa Trypanosoma cruzi Salinas, E. Torriero, A.A.J. Battaglini, F. Sanz, M.I. Olsina, R. Raba, J. Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease |
| topic_facet |
Biosensors Flow system Horseradish peroxidase Immunoassays Osmium Trypanosoma cruzi Antibodies Bioassay Complexation Diseases Enzymes Hydrogen peroxide Immunology Osmium compounds Protozoa Spectrophotometry Chagas disease Horseradisg peroxidase (HRP) enzymes Immunoassays Serum Bioreactors horseradish peroxidase human immunoglobulin hydrogen peroxide immobilized antibody immunoglobulin G parasite antibody parasite antigen amperometry analytical error antibody labeling antigen antibody reaction article bioreactor bioreactor design Chagas disease continuous flow reactor enzyme immunoassay parasite identification quantitative analysis reproducibility spectrophotometry Trypanosoma cruzi Animals Antibodies, Protozoan Bioreactors Chagas Disease Equipment Design Equipment Failure Analysis Flow Injection Analysis Humans Immunoenzyme Techniques Rotation Trypanosoma cruzi Armoracia rusticana Protozoa Trypanosoma cruzi |
| description |
The high sensitivity that can be attained using an immunoassays coupled to a rotating bioreactor with electrochemical detection mediated by [Os(bpy)2Cl(pyCOOH)]Cl, has been verified for the detection of Trypanozoma cruzi (T. cruzi), This protozoan parasite causes Chagas disease, affecting more than 18 million people in central and south America. Antibodies in the serum sample are allowed to react immunologically with whole homogenates of the parasite as antigen that are immobilized on a rotating disk. The bound antibodies are quantified by a horseradish peroxidase (HRP) enzyme labeled second antibodies specific to human IgG in presence of hydrogen peroxide using an osmium complex [Os(bpy)2Cl(pyCOOH)]Cl as enzymatic mediators. The amperometric measurement performed at 0.00 V versus Ag/AgCl can be done within 2 min and the analysis time does not exceed 23 min. The calculated detection limits was 0.01 mIU ml-1. Reproducibility assays were made using repetitive serum of 0.182 mIU ml-1 T. cruzi specific antibody (measured as the activity of the correspondent anti-serum's enzyme conjugated); the percentage standard error was less than 5%. The amperometric immunoreactors showed significantly higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method. © 2004 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Salinas, E. Torriero, A.A.J. Battaglini, F. Sanz, M.I. Olsina, R. Raba, J. |
| author_facet |
Salinas, E. Torriero, A.A.J. Battaglini, F. Sanz, M.I. Olsina, R. Raba, J. |
| author_sort |
Salinas, E. |
| title |
Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease |
| title_short |
Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease |
| title_full |
Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease |
| title_fullStr |
Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease |
| title_full_unstemmed |
Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease |
| title_sort |
continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: determination of chagas disease |
| url |
http://hdl.handle.net/20.500.12110/paper_09565663_v21_n2_p313_Salinas |
| work_keys_str_mv |
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1807317988728111104 |