Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes
Multilayer immobilization of antibody and redox polymer molecules on a gold electrode was achieved, as a strategy for the potential development of an amperometric immunosensor. The step-by-step assembly of antibiotin IgG on Os(bpy)2ClPyCH2NH poly(allylamine) redox polymer (PAH-Os) adsorbed on thiola...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09565663_v19_n10_p1219_Calvo |
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todo:paper_09565663_v19_n10_p1219_Calvo2023-10-03T15:52:08Z Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes Calvo, E.J. Danilowicz, C. Lagier, C.M. Manrique, J. Otero, M. IgG Immunoassays Immunoelectrodes Redox polymer Self-assembled Atomic force microscopy Deposition Electrodes Gold Polymers Self assembly Conjugate binding Electrocatalysis Biosensors antibody avidin biotin gold complex immobilized antibody immunoglobulin G peroxidase polymer adsorption amperometric biosensor analytic method article atomic force microscopy catalysis catalyst chemical reaction conjugation crystalloid electrochemistry electrode molecular recognition oxidation reduction reaction Antibodies Electrochemistry Electrodes Gold Immunoassay Kinetics Microscopy, Atomic Force Oxidation-Reduction Polymers Armoracia rusticana Multilayer immobilization of antibody and redox polymer molecules on a gold electrode was achieved, as a strategy for the potential development of an amperometric immunosensor. The step-by-step assembly of antibiotin IgG on Os(bpy)2ClPyCH2NH poly(allylamine) redox polymer (PAH-Os) adsorbed on thiolated gold electrodes was proved by quartz crystal microbalance (QCM) and atomic force microscopy (AFM) experiments, confirming the electrochemical evidence. The increase of redox charge during the layer-by-layer deposition demonstrated that charge propagation within the layers is feasible. The multilayer structure proved to be effective for the molecular recognition of horseradish peroxidase-biotin conjugate (HRP-biotin), as confirmed by the QCM measurements and the electrocatalytic reduction current obtained upon H2O2 addition. The catalytic current resulting from PAH-Os mediation was shown to increase with the number of assembled layers. Furthermore, the inventory of IgG molecules on the supramolecular self-assembled structure and the specific and non-specific binding of HRP-biotin conjugate were confirmed by the QCM transient studies, giving information on the kinetics of IgG deposition and HRP-biotin conjugate binding to the IgG. © 2003 Elsevier B.V. All rights reserved. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Otero, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09565663_v19_n10_p1219_Calvo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
IgG Immunoassays Immunoelectrodes Redox polymer Self-assembled Atomic force microscopy Deposition Electrodes Gold Polymers Self assembly Conjugate binding Electrocatalysis Biosensors antibody avidin biotin gold complex immobilized antibody immunoglobulin G peroxidase polymer adsorption amperometric biosensor analytic method article atomic force microscopy catalysis catalyst chemical reaction conjugation crystalloid electrochemistry electrode molecular recognition oxidation reduction reaction Antibodies Electrochemistry Electrodes Gold Immunoassay Kinetics Microscopy, Atomic Force Oxidation-Reduction Polymers Armoracia rusticana |
| spellingShingle |
IgG Immunoassays Immunoelectrodes Redox polymer Self-assembled Atomic force microscopy Deposition Electrodes Gold Polymers Self assembly Conjugate binding Electrocatalysis Biosensors antibody avidin biotin gold complex immobilized antibody immunoglobulin G peroxidase polymer adsorption amperometric biosensor analytic method article atomic force microscopy catalysis catalyst chemical reaction conjugation crystalloid electrochemistry electrode molecular recognition oxidation reduction reaction Antibodies Electrochemistry Electrodes Gold Immunoassay Kinetics Microscopy, Atomic Force Oxidation-Reduction Polymers Armoracia rusticana Calvo, E.J. Danilowicz, C. Lagier, C.M. Manrique, J. Otero, M. Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
| topic_facet |
IgG Immunoassays Immunoelectrodes Redox polymer Self-assembled Atomic force microscopy Deposition Electrodes Gold Polymers Self assembly Conjugate binding Electrocatalysis Biosensors antibody avidin biotin gold complex immobilized antibody immunoglobulin G peroxidase polymer adsorption amperometric biosensor analytic method article atomic force microscopy catalysis catalyst chemical reaction conjugation crystalloid electrochemistry electrode molecular recognition oxidation reduction reaction Antibodies Electrochemistry Electrodes Gold Immunoassay Kinetics Microscopy, Atomic Force Oxidation-Reduction Polymers Armoracia rusticana |
| description |
Multilayer immobilization of antibody and redox polymer molecules on a gold electrode was achieved, as a strategy for the potential development of an amperometric immunosensor. The step-by-step assembly of antibiotin IgG on Os(bpy)2ClPyCH2NH poly(allylamine) redox polymer (PAH-Os) adsorbed on thiolated gold electrodes was proved by quartz crystal microbalance (QCM) and atomic force microscopy (AFM) experiments, confirming the electrochemical evidence. The increase of redox charge during the layer-by-layer deposition demonstrated that charge propagation within the layers is feasible. The multilayer structure proved to be effective for the molecular recognition of horseradish peroxidase-biotin conjugate (HRP-biotin), as confirmed by the QCM measurements and the electrocatalytic reduction current obtained upon H2O2 addition. The catalytic current resulting from PAH-Os mediation was shown to increase with the number of assembled layers. Furthermore, the inventory of IgG molecules on the supramolecular self-assembled structure and the specific and non-specific binding of HRP-biotin conjugate were confirmed by the QCM transient studies, giving information on the kinetics of IgG deposition and HRP-biotin conjugate binding to the IgG. © 2003 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Calvo, E.J. Danilowicz, C. Lagier, C.M. Manrique, J. Otero, M. |
| author_facet |
Calvo, E.J. Danilowicz, C. Lagier, C.M. Manrique, J. Otero, M. |
| author_sort |
Calvo, E.J. |
| title |
Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
| title_short |
Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
| title_full |
Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
| title_fullStr |
Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
| title_full_unstemmed |
Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
| title_sort |
characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
| url |
http://hdl.handle.net/20.500.12110/paper_09565663_v19_n10_p1219_Calvo |
| work_keys_str_mv |
AT calvoej characterizationofselfassembledredoxpolymerandantibodymoleculesonthiolatedgoldelectrodes AT danilowiczc characterizationofselfassembledredoxpolymerandantibodymoleculesonthiolatedgoldelectrodes AT lagiercm characterizationofselfassembledredoxpolymerandantibodymoleculesonthiolatedgoldelectrodes AT manriquej characterizationofselfassembledredoxpolymerandantibodymoleculesonthiolatedgoldelectrodes AT oterom characterizationofselfassembledredoxpolymerandantibodymoleculesonthiolatedgoldelectrodes |
| _version_ |
1807320918886711296 |