Mechanism behind gamma band activity in the pedunculopontine nucleus
The pedunculopontine nucleus (PPN), part of the reticular activating system, modulates waking and paradoxical sleep. During waking and paradoxical sleep, EEG responses are characterized by low-amplitude, high-frequency oscillatory activity in the beta-gamma band range (∼20-80Hz). We have previously...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0953816X_v34_n3_p404_Kezunovic |
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todo:paper_0953816X_v34_n3_p404_Kezunovic2023-10-03T15:51:19Z Mechanism behind gamma band activity in the pedunculopontine nucleus Kezunovic, N. Urbano, F.J. Simon, C. Hyde, J. Smith, K. Garcia-Rill, E. Arousal Calcium channels Gamma band N-type Oscillations P/Q-type calcium channel N type calcium channel P type calcium channel Q type potassium channel voltage gated calcium channel animal cell animal tissue article cholinergic transmission controlled study electric potential electroencephalography membrane depolarization membrane potential membrane steady potential neurotransmission nonhuman oscillation pedunculopontine tegmental nucleus priority journal rat signal transduction synapse voltage clamp Animals Calcium Channels, N-Type Carbachol Cholinergic Agonists Electroencephalography Membrane Potentials Neurons omega-Agatoxin IVA omega-Conotoxin GVIA Patch-Clamp Techniques Pedunculopontine Tegmental Nucleus Peptides Potassium Channels, Voltage-Gated Rats Rats, Sprague-Dawley Sleep Sodium Channel Blockers Tetrodotoxin The pedunculopontine nucleus (PPN), part of the reticular activating system, modulates waking and paradoxical sleep. During waking and paradoxical sleep, EEG responses are characterized by low-amplitude, high-frequency oscillatory activity in the beta-gamma band range (∼20-80Hz). We have previously reported that gamma band activity may be intrinsically generated by the membrane electroresponsiveness of PPN neurons, and that the neuronal ensemble generates different patterns of gamma activity in response to specific transmitters. This study attempted to identify the voltage-gated calcium and potassium channels involved in the rising and falling phases of gamma oscillations in PPN neurons. We found that all rat (8-14day) PPN cell types showed gamma oscillations in the presence of TTX and synaptic blockers when membrane potential was depolarized using current ramps. PPN neurons showed gamma oscillations when voltage-clamped at holding potentials above -30mV, suggesting that their origin may be spatially located beyond voltage-clamp control. The average frequency for all PPN cell types was 23±1Hz and this increased under carbachol (47±2Hz; anova df=64, t=12.5, P<0.001). The N-type calcium channel blocker ω-conotoxin-GVIA partially reduced gamma oscillations, while the P/Q-type blocker ω-agatoxin-IVA abolished them. Both ω-CgTX and ω-Aga blocked voltage-dependent calcium currents, by 56 and 52% respectively. The delayed rectifier-like potassium channel blocker α-dendrotoxin also abolished gamma oscillations. In carbachol-induced PPN population responses, ω-agatoxin-IVA reduced higher, and ω-CgTx mostly lower, frequencies. These results suggest that voltage-dependent P/Q- and, to a lesser extent, N-type calcium channels mediate gamma oscillations in PPN. © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0953816X_v34_n3_p404_Kezunovic |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arousal Calcium channels Gamma band N-type Oscillations P/Q-type calcium channel N type calcium channel P type calcium channel Q type potassium channel voltage gated calcium channel animal cell animal tissue article cholinergic transmission controlled study electric potential electroencephalography membrane depolarization membrane potential membrane steady potential neurotransmission nonhuman oscillation pedunculopontine tegmental nucleus priority journal rat signal transduction synapse voltage clamp Animals Calcium Channels, N-Type Carbachol Cholinergic Agonists Electroencephalography Membrane Potentials Neurons omega-Agatoxin IVA omega-Conotoxin GVIA Patch-Clamp Techniques Pedunculopontine Tegmental Nucleus Peptides Potassium Channels, Voltage-Gated Rats Rats, Sprague-Dawley Sleep Sodium Channel Blockers Tetrodotoxin |
| spellingShingle |
Arousal Calcium channels Gamma band N-type Oscillations P/Q-type calcium channel N type calcium channel P type calcium channel Q type potassium channel voltage gated calcium channel animal cell animal tissue article cholinergic transmission controlled study electric potential electroencephalography membrane depolarization membrane potential membrane steady potential neurotransmission nonhuman oscillation pedunculopontine tegmental nucleus priority journal rat signal transduction synapse voltage clamp Animals Calcium Channels, N-Type Carbachol Cholinergic Agonists Electroencephalography Membrane Potentials Neurons omega-Agatoxin IVA omega-Conotoxin GVIA Patch-Clamp Techniques Pedunculopontine Tegmental Nucleus Peptides Potassium Channels, Voltage-Gated Rats Rats, Sprague-Dawley Sleep Sodium Channel Blockers Tetrodotoxin Kezunovic, N. Urbano, F.J. Simon, C. Hyde, J. Smith, K. Garcia-Rill, E. Mechanism behind gamma band activity in the pedunculopontine nucleus |
| topic_facet |
Arousal Calcium channels Gamma band N-type Oscillations P/Q-type calcium channel N type calcium channel P type calcium channel Q type potassium channel voltage gated calcium channel animal cell animal tissue article cholinergic transmission controlled study electric potential electroencephalography membrane depolarization membrane potential membrane steady potential neurotransmission nonhuman oscillation pedunculopontine tegmental nucleus priority journal rat signal transduction synapse voltage clamp Animals Calcium Channels, N-Type Carbachol Cholinergic Agonists Electroencephalography Membrane Potentials Neurons omega-Agatoxin IVA omega-Conotoxin GVIA Patch-Clamp Techniques Pedunculopontine Tegmental Nucleus Peptides Potassium Channels, Voltage-Gated Rats Rats, Sprague-Dawley Sleep Sodium Channel Blockers Tetrodotoxin |
| description |
The pedunculopontine nucleus (PPN), part of the reticular activating system, modulates waking and paradoxical sleep. During waking and paradoxical sleep, EEG responses are characterized by low-amplitude, high-frequency oscillatory activity in the beta-gamma band range (∼20-80Hz). We have previously reported that gamma band activity may be intrinsically generated by the membrane electroresponsiveness of PPN neurons, and that the neuronal ensemble generates different patterns of gamma activity in response to specific transmitters. This study attempted to identify the voltage-gated calcium and potassium channels involved in the rising and falling phases of gamma oscillations in PPN neurons. We found that all rat (8-14day) PPN cell types showed gamma oscillations in the presence of TTX and synaptic blockers when membrane potential was depolarized using current ramps. PPN neurons showed gamma oscillations when voltage-clamped at holding potentials above -30mV, suggesting that their origin may be spatially located beyond voltage-clamp control. The average frequency for all PPN cell types was 23±1Hz and this increased under carbachol (47±2Hz; anova df=64, t=12.5, P<0.001). The N-type calcium channel blocker ω-conotoxin-GVIA partially reduced gamma oscillations, while the P/Q-type blocker ω-agatoxin-IVA abolished them. Both ω-CgTX and ω-Aga blocked voltage-dependent calcium currents, by 56 and 52% respectively. The delayed rectifier-like potassium channel blocker α-dendrotoxin also abolished gamma oscillations. In carbachol-induced PPN population responses, ω-agatoxin-IVA reduced higher, and ω-CgTx mostly lower, frequencies. These results suggest that voltage-dependent P/Q- and, to a lesser extent, N-type calcium channels mediate gamma oscillations in PPN. © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd. |
| format |
JOUR |
| author |
Kezunovic, N. Urbano, F.J. Simon, C. Hyde, J. Smith, K. Garcia-Rill, E. |
| author_facet |
Kezunovic, N. Urbano, F.J. Simon, C. Hyde, J. Smith, K. Garcia-Rill, E. |
| author_sort |
Kezunovic, N. |
| title |
Mechanism behind gamma band activity in the pedunculopontine nucleus |
| title_short |
Mechanism behind gamma band activity in the pedunculopontine nucleus |
| title_full |
Mechanism behind gamma band activity in the pedunculopontine nucleus |
| title_fullStr |
Mechanism behind gamma band activity in the pedunculopontine nucleus |
| title_full_unstemmed |
Mechanism behind gamma band activity in the pedunculopontine nucleus |
| title_sort |
mechanism behind gamma band activity in the pedunculopontine nucleus |
| url |
http://hdl.handle.net/20.500.12110/paper_0953816X_v34_n3_p404_Kezunovic |
| work_keys_str_mv |
AT kezunovicn mechanismbehindgammabandactivityinthepedunculopontinenucleus AT urbanofj mechanismbehindgammabandactivityinthepedunculopontinenucleus AT simonc mechanismbehindgammabandactivityinthepedunculopontinenucleus AT hydej mechanismbehindgammabandactivityinthepedunculopontinenucleus AT smithk mechanismbehindgammabandactivityinthepedunculopontinenucleus AT garciarille mechanismbehindgammabandactivityinthepedunculopontinenucleus |
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