rDNA analyses as an aid to the taxonomy of species of Ganoderma

As part of research to implement methods for the identification of Ganoderma species, this paper describes the use of PCR coupled to restriction digestions, single-strand conformational polymorphism (SSCP) and direct sequencing to assay rDNA polymorphism in South American collections of Ganoderma. T...

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Detalles Bibliográficos
Autores principales:	Gottlieb, A.M., Ferrer, E., Wright, J.E.
Formato:	JOUR
Materias:	DNA polymorphism genetic variance internal transcribed spacer nucleotide sequence polymerase chain reaction ribosomal DNA single strand conformational polymorphism South America fungus taxonomy Ganoderma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09537562_v104_n9_p1033_Gottlieb
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09537562_v104_n9_p1033_Gottlieb
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spelling	todo:paper_09537562_v104_n9_p1033_Gottlieb2023-10-03T15:51:11Z rDNA analyses as an aid to the taxonomy of species of Ganoderma Gottlieb, A.M. Ferrer, E. Wright, J.E. DNA polymorphism genetic variance internal transcribed spacer nucleotide sequence polymerase chain reaction ribosomal DNA single strand conformational polymorphism South America fungus polymerase chain reaction taxonomy Ganoderma As part of research to implement methods for the identification of Ganoderma species, this paper describes the use of PCR coupled to restriction digestions, single-strand conformational polymorphism (SSCP) and direct sequencing to assay rDNA polymorphism in South American collections of Ganoderma. The aim of the present study was to determine whether morphologically defined groups could be discriminated by molecular markers and to examine ITS sequence variation. Nineteen isolates of subgenus Ganoderma representing seven morphological taxa, and thirty isolates of subgenus Elfvingia representing three taxa, were studied. ITS I and IT II regions of twenty-one isolates were sequenced and were cladistically analyzed. Seven additional sequences were downloaded from EMBL/Genbank for comparison. In general, agreement between groupings delimited by restriction patterns, SSCP of ITS I and ITS II was evident. Gene trees derived from ITS I, ITS II and from a combined data set, were obtained and compared. Agreement between molecular and morphological data was clear at the subgeneric level; however, at the specific level, this relationship was difficult to visualize. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09537562_v104_n9_p1033_Gottlieb
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	DNA polymorphism genetic variance internal transcribed spacer nucleotide sequence polymerase chain reaction ribosomal DNA single strand conformational polymorphism South America fungus polymerase chain reaction taxonomy Ganoderma
spellingShingle	DNA polymorphism genetic variance internal transcribed spacer nucleotide sequence polymerase chain reaction ribosomal DNA single strand conformational polymorphism South America fungus polymerase chain reaction taxonomy Ganoderma Gottlieb, A.M. Ferrer, E. Wright, J.E. rDNA analyses as an aid to the taxonomy of species of Ganoderma
topic_facet	DNA polymorphism genetic variance internal transcribed spacer nucleotide sequence polymerase chain reaction ribosomal DNA single strand conformational polymorphism South America fungus polymerase chain reaction taxonomy Ganoderma
description	As part of research to implement methods for the identification of Ganoderma species, this paper describes the use of PCR coupled to restriction digestions, single-strand conformational polymorphism (SSCP) and direct sequencing to assay rDNA polymorphism in South American collections of Ganoderma. The aim of the present study was to determine whether morphologically defined groups could be discriminated by molecular markers and to examine ITS sequence variation. Nineteen isolates of subgenus Ganoderma representing seven morphological taxa, and thirty isolates of subgenus Elfvingia representing three taxa, were studied. ITS I and IT II regions of twenty-one isolates were sequenced and were cladistically analyzed. Seven additional sequences were downloaded from EMBL/Genbank for comparison. In general, agreement between groupings delimited by restriction patterns, SSCP of ITS I and ITS II was evident. Gene trees derived from ITS I, ITS II and from a combined data set, were obtained and compared. Agreement between molecular and morphological data was clear at the subgeneric level; however, at the specific level, this relationship was difficult to visualize.
format	JOUR
author	Gottlieb, A.M. Ferrer, E. Wright, J.E.
author_facet	Gottlieb, A.M. Ferrer, E. Wright, J.E.
author_sort	Gottlieb, A.M.
title	rDNA analyses as an aid to the taxonomy of species of Ganoderma
title_short	rDNA analyses as an aid to the taxonomy of species of Ganoderma
title_full	rDNA analyses as an aid to the taxonomy of species of Ganoderma
title_fullStr	rDNA analyses as an aid to the taxonomy of species of Ganoderma
title_full_unstemmed	rDNA analyses as an aid to the taxonomy of species of Ganoderma
title_sort	rdna analyses as an aid to the taxonomy of species of ganoderma
url	http://hdl.handle.net/20.500.12110/paper_09537562_v104_n9_p1033_Gottlieb
work_keys_str_mv	AT gottliebam rdnaanalysesasanaidtothetaxonomyofspeciesofganoderma AT ferrere rdnaanalysesasanaidtothetaxonomyofspeciesofganoderma AT wrightje rdnaanalysesasanaidtothetaxonomyofspeciesofganoderma
_version_	1782030194165415936

rDNA analyses as an aid to the taxonomy of species of Ganoderma

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