Real-time reaction monitoring by probe electrospray ionization mass spectrometry
Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09514198_v24_n11_p1507_Yu |
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todo:paper_09514198_v24_n11_p1507_Yu2023-10-03T15:50:41Z Real-time reaction monitoring by probe electrospray ionization mass spectrometry Yu, Z. Chen, L.C. Erra-Balsells, R. Nonami, H. Hiraoka, K. myoglobin peptide article chemical reaction chemical structure chemistry computer system electrospray mass spectrometry evaluation human methodology protein denaturation Chemical Processes Computer Systems Humans Molecular Structure Myoglobin Peptides Protein Denaturation Spectrometry, Mass, Electrospray Ionization Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption. © 2010 John Wiley & Sons, Ltd. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09514198_v24_n11_p1507_Yu |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
myoglobin peptide article chemical reaction chemical structure chemistry computer system electrospray mass spectrometry evaluation human methodology protein denaturation Chemical Processes Computer Systems Humans Molecular Structure Myoglobin Peptides Protein Denaturation Spectrometry, Mass, Electrospray Ionization |
| spellingShingle |
myoglobin peptide article chemical reaction chemical structure chemistry computer system electrospray mass spectrometry evaluation human methodology protein denaturation Chemical Processes Computer Systems Humans Molecular Structure Myoglobin Peptides Protein Denaturation Spectrometry, Mass, Electrospray Ionization Yu, Z. Chen, L.C. Erra-Balsells, R. Nonami, H. Hiraoka, K. Real-time reaction monitoring by probe electrospray ionization mass spectrometry |
| topic_facet |
myoglobin peptide article chemical reaction chemical structure chemistry computer system electrospray mass spectrometry evaluation human methodology protein denaturation Chemical Processes Computer Systems Humans Molecular Structure Myoglobin Peptides Protein Denaturation Spectrometry, Mass, Electrospray Ionization |
| description |
Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption. © 2010 John Wiley & Sons, Ltd. |
| format |
JOUR |
| author |
Yu, Z. Chen, L.C. Erra-Balsells, R. Nonami, H. Hiraoka, K. |
| author_facet |
Yu, Z. Chen, L.C. Erra-Balsells, R. Nonami, H. Hiraoka, K. |
| author_sort |
Yu, Z. |
| title |
Real-time reaction monitoring by probe electrospray ionization mass spectrometry |
| title_short |
Real-time reaction monitoring by probe electrospray ionization mass spectrometry |
| title_full |
Real-time reaction monitoring by probe electrospray ionization mass spectrometry |
| title_fullStr |
Real-time reaction monitoring by probe electrospray ionization mass spectrometry |
| title_full_unstemmed |
Real-time reaction monitoring by probe electrospray ionization mass spectrometry |
| title_sort |
real-time reaction monitoring by probe electrospray ionization mass spectrometry |
| url |
http://hdl.handle.net/20.500.12110/paper_09514198_v24_n11_p1507_Yu |
| work_keys_str_mv |
AT yuz realtimereactionmonitoringbyprobeelectrosprayionizationmassspectrometry AT chenlc realtimereactionmonitoringbyprobeelectrosprayionizationmassspectrometry AT errabalsellsr realtimereactionmonitoringbyprobeelectrosprayionizationmassspectrometry AT nonamih realtimereactionmonitoringbyprobeelectrosprayionizationmassspectrometry AT hiraokak realtimereactionmonitoringbyprobeelectrosprayionizationmassspectrometry |
| _version_ |
1782024121296617472 |