Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex
The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative specie...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09498257_v23_n6_p903_Ramos |
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todo:paper_09498257_v23_n6_p903_Ramos2023-10-03T15:49:36Z Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex Ramos, L.C.B. Rodrigues, F.P. Biazzotto, J.C. de Paula Machado, S. Slep, L.D. Hamblin, M.R. da Silva, R.S. Conjugated ruthenium-antibody complex Nitric oxide delivery agent Nitrosyl ruthenium complexes 2,2' bipyridine derivative antibody drug conjugate cell surface marker dicarboxylic acid derivative immunoglobulin G antibody nitric oxide ruthenium complex voltage dependent anion channel animal cell animal experiment animal tissue antineoplastic activity Article cell surface comparative study controlled study cytotoxicity density functional theory drug targeting Hep-G2 cell line liver cell carcinoma male nonhuman priority journal protein targeting rat The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release. © 2018, SBIC. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09498257_v23_n6_p903_Ramos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Conjugated ruthenium-antibody complex Nitric oxide delivery agent Nitrosyl ruthenium complexes 2,2' bipyridine derivative antibody drug conjugate cell surface marker dicarboxylic acid derivative immunoglobulin G antibody nitric oxide ruthenium complex voltage dependent anion channel animal cell animal experiment animal tissue antineoplastic activity Article cell surface comparative study controlled study cytotoxicity density functional theory drug targeting Hep-G2 cell line liver cell carcinoma male nonhuman priority journal protein targeting rat |
| spellingShingle |
Conjugated ruthenium-antibody complex Nitric oxide delivery agent Nitrosyl ruthenium complexes 2,2' bipyridine derivative antibody drug conjugate cell surface marker dicarboxylic acid derivative immunoglobulin G antibody nitric oxide ruthenium complex voltage dependent anion channel animal cell animal experiment animal tissue antineoplastic activity Article cell surface comparative study controlled study cytotoxicity density functional theory drug targeting Hep-G2 cell line liver cell carcinoma male nonhuman priority journal protein targeting rat Ramos, L.C.B. Rodrigues, F.P. Biazzotto, J.C. de Paula Machado, S. Slep, L.D. Hamblin, M.R. da Silva, R.S. Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
| topic_facet |
Conjugated ruthenium-antibody complex Nitric oxide delivery agent Nitrosyl ruthenium complexes 2,2' bipyridine derivative antibody drug conjugate cell surface marker dicarboxylic acid derivative immunoglobulin G antibody nitric oxide ruthenium complex voltage dependent anion channel animal cell animal experiment animal tissue antineoplastic activity Article cell surface comparative study controlled study cytotoxicity density functional theory drug targeting Hep-G2 cell line liver cell carcinoma male nonhuman priority journal protein targeting rat |
| description |
The rational design of anti-cancer agents includes a new approach based on ruthenium complexes that can act as nitric oxide (NO) donor agents against specific cellular targets. One of the most studied classes of those compounds is based on bis(bipyridine) ruthenium fragment and its derivative species. In this work, we present the chemical and cytotoxicity properties against the liver hepatocellular carcinoma cell line HepG2 of cis-[RuII(NO+)Cl(dcbpy)2]2− conjugated to a polyclonal antibody IgG (anti-VDAC) recognizing a cell surface marker. UV–visible bands of the ruthenium complex were assigned with the aid of density functional theory, which also allowed estimation of the structures that explain the biological effects of the ruthenium complex–IgG conjugate. The interaction of cis-[RuII(NO+)Cl(dcbpy)2]3− with mitochondria was evaluated due to the potential of these organelles as anti-cancer targets, and considering they interact with the anti-VDAC antibody. The cytotoxicity of cis-[RuII(NO+)Cl(dcbpy)2]3−-anti-VDAC antibody was up to 80% greater in comparison to the free cis-[RuII(NO+)Cl(dcbpy)2]3− complex. We suggest that this effect is due to site-specific interaction of the complex followed by NO release. © 2018, SBIC. |
| format |
JOUR |
| author |
Ramos, L.C.B. Rodrigues, F.P. Biazzotto, J.C. de Paula Machado, S. Slep, L.D. Hamblin, M.R. da Silva, R.S. |
| author_facet |
Ramos, L.C.B. Rodrigues, F.P. Biazzotto, J.C. de Paula Machado, S. Slep, L.D. Hamblin, M.R. da Silva, R.S. |
| author_sort |
Ramos, L.C.B. |
| title |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
| title_short |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
| title_full |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
| title_fullStr |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
| title_full_unstemmed |
Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
| title_sort |
targeting the mitochondrial vdac in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex |
| url |
http://hdl.handle.net/20.500.12110/paper_09498257_v23_n6_p903_Ramos |
| work_keys_str_mv |
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| _version_ |
1782023787745640448 |