Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas
Estrogens are considered to be critically involved in lactotroph and lactosomatotroph pituitary tumor development. In addition to direct effects, estradiol-induced tumor formation may involve alterations in growth factor and cytokine production. We have studied whether estradiol stimulates the produ...
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todo:paper_09477349_v112_n1_p18_Onofri2023-10-03T15:49:32Z Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas Onofri, C. Carbia Nagashima, A. Schaaf, L. Feirer, M. Lohrer, P. Stummer, W. Berner, S. Chervin, A. Goldberg, V. Stalla, G.K. Renner, U. Arzt, E. Estrogens Interleukin-6 Lactotroph/lactosomatotroph adenomas Vascular endothelial growth factor estradiol interleukin 6 vasculotropin adult article clinical article cytokine production female hormonal regulation human human tissue hypophysis adenoma male neuroendocrinology priority journal protein expression Adult Enzyme-Linked Immunosorbent Assay Estradiol Female Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Prolactinoma Tumor Cells, Cultured Vascular Endothelial Growth Factor A Estrogens are considered to be critically involved in lactotroph and lactosomatotroph pituitary tumor development. In addition to direct effects, estradiol-induced tumor formation may involve alterations in growth factor and cytokine production. We have studied whether estradiol stimulates the production of the angiogenic vascular endothelial growth factor and the potential tumor progression factor interleukin-6 in 5 lactotroph (LA) and 5 lactosomatotroph (LSA) human pituitary adenoma cell cultures. All tumors secreted heterogenous basal amounts of VEGF (18.0 ± 1.4 to 425 ± 26 pg/ml per 24h) and IL-6 (18.1 ± 1.5 to 604 ± 17 pg/ml per 24h). Estradiol (100nM) significantly enhanced VEGF release in all LA and LSA cell cultures (47 to 168% above basal). IL-6 secretion was stimulated in 3 out of 5 LA and in all LSA cell cultures (31 to 287% above basal). In cell cultures obtained from tumors from which sufficient cells could be isolated, a dose-dependent effect of estradiol (1 to 100 nM) on VEGF and IL-6 production was observed. Stimulation of IL-6 and/or VEGF secretion by estradiol in the majority of human lactotroph and lactosomatotroph adenoma cell cultures studied, suggests that estrogens may contribute to adenoma expansion through the stimulation of these auto-/paracrine-acting adenoma progression factors. Fil:Carbia Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09477349_v112_n1_p18_Onofri |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Estrogens Interleukin-6 Lactotroph/lactosomatotroph adenomas Vascular endothelial growth factor estradiol interleukin 6 vasculotropin adult article clinical article cytokine production female hormonal regulation human human tissue hypophysis adenoma male neuroendocrinology priority journal protein expression Adult Enzyme-Linked Immunosorbent Assay Estradiol Female Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Prolactinoma Tumor Cells, Cultured Vascular Endothelial Growth Factor A |
| spellingShingle |
Estrogens Interleukin-6 Lactotroph/lactosomatotroph adenomas Vascular endothelial growth factor estradiol interleukin 6 vasculotropin adult article clinical article cytokine production female hormonal regulation human human tissue hypophysis adenoma male neuroendocrinology priority journal protein expression Adult Enzyme-Linked Immunosorbent Assay Estradiol Female Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Prolactinoma Tumor Cells, Cultured Vascular Endothelial Growth Factor A Onofri, C. Carbia Nagashima, A. Schaaf, L. Feirer, M. Lohrer, P. Stummer, W. Berner, S. Chervin, A. Goldberg, V. Stalla, G.K. Renner, U. Arzt, E. Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas |
| topic_facet |
Estrogens Interleukin-6 Lactotroph/lactosomatotroph adenomas Vascular endothelial growth factor estradiol interleukin 6 vasculotropin adult article clinical article cytokine production female hormonal regulation human human tissue hypophysis adenoma male neuroendocrinology priority journal protein expression Adult Enzyme-Linked Immunosorbent Assay Estradiol Female Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Prolactinoma Tumor Cells, Cultured Vascular Endothelial Growth Factor A |
| description |
Estrogens are considered to be critically involved in lactotroph and lactosomatotroph pituitary tumor development. In addition to direct effects, estradiol-induced tumor formation may involve alterations in growth factor and cytokine production. We have studied whether estradiol stimulates the production of the angiogenic vascular endothelial growth factor and the potential tumor progression factor interleukin-6 in 5 lactotroph (LA) and 5 lactosomatotroph (LSA) human pituitary adenoma cell cultures. All tumors secreted heterogenous basal amounts of VEGF (18.0 ± 1.4 to 425 ± 26 pg/ml per 24h) and IL-6 (18.1 ± 1.5 to 604 ± 17 pg/ml per 24h). Estradiol (100nM) significantly enhanced VEGF release in all LA and LSA cell cultures (47 to 168% above basal). IL-6 secretion was stimulated in 3 out of 5 LA and in all LSA cell cultures (31 to 287% above basal). In cell cultures obtained from tumors from which sufficient cells could be isolated, a dose-dependent effect of estradiol (1 to 100 nM) on VEGF and IL-6 production was observed. Stimulation of IL-6 and/or VEGF secretion by estradiol in the majority of human lactotroph and lactosomatotroph adenoma cell cultures studied, suggests that estrogens may contribute to adenoma expansion through the stimulation of these auto-/paracrine-acting adenoma progression factors. |
| format |
JOUR |
| author |
Onofri, C. Carbia Nagashima, A. Schaaf, L. Feirer, M. Lohrer, P. Stummer, W. Berner, S. Chervin, A. Goldberg, V. Stalla, G.K. Renner, U. Arzt, E. |
| author_facet |
Onofri, C. Carbia Nagashima, A. Schaaf, L. Feirer, M. Lohrer, P. Stummer, W. Berner, S. Chervin, A. Goldberg, V. Stalla, G.K. Renner, U. Arzt, E. |
| author_sort |
Onofri, C. |
| title |
Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas |
| title_short |
Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas |
| title_full |
Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas |
| title_fullStr |
Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas |
| title_full_unstemmed |
Estradiol Stimulates Vascular Endothelial Growth Factor and Interleukin-6 in Human Lactotroph and Lactosomatotroph Pituitary Adenomas |
| title_sort |
estradiol stimulates vascular endothelial growth factor and interleukin-6 in human lactotroph and lactosomatotroph pituitary adenomas |
| url |
http://hdl.handle.net/20.500.12110/paper_09477349_v112_n1_p18_Onofri |
| work_keys_str_mv |
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