Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus

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A purified sulfated xylomannan, named F6, obtained from the red seaweed Nothogenia fastigiata, proved to be a potent inhibitor of HSV-1 in vitro without affecting cell viability. In a virus yield reduction assay, the inhibitory concentration 50% (IC50) was 0.66 μg/ml. The mode of action of F6 was as...

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Autores principales: Pujol, C.A., Matulewicz, M.C., Cerezo, A.S., Damonte, E.B.
Formato: JOUR
Materias:
Glycoprotein
Hemagglutination inhibition
Herpes simplex virus
Natural antiviral
Rosette inhibition
Sulfated polysaccharide
algae
Herpes
Human herpesvirus 1
Nothogenia fastigiata
Ovis aries
Simplexvirus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09447113_v5_n3_p205_Pujol
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09447113_v5_n3_p205_Pujol
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spelling todo:paper_09447113_v5_n3_p205_Pujol2023-10-03T15:49:16Z Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus Pujol, C.A. Matulewicz, M.C. Cerezo, A.S. Damonte, E.B. Glycoprotein Hemagglutination inhibition Herpes simplex virus Natural antiviral Rosette inhibition Sulfated polysaccharide algae Herpes Human herpesvirus 1 Nothogenia fastigiata Ovis aries Simplexvirus A purified sulfated xylomannan, named F6, obtained from the red seaweed Nothogenia fastigiata, proved to be a potent inhibitor of HSV-1 in vitro without affecting cell viability. In a virus yield reduction assay, the inhibitory concentration 50% (IC50) was 0.66 μg/ml. The mode of action of F6 was ascribed to an inhibitory effect on virus adsorption. The glycoprotein C (gC) of HSV-1 is involved in virus binding to the cell surface heparan sulfate. Furthermore, it mediates other biological activities such as induction of hemagglutination and binding to the third component of complement C3b. The compound F6 was effective in inhibiting hemagglutination induced by HSV-1 and also causes a reduction of 50% in rosette formation between sheep red blood cells coupled to C3b and cells infected with HSV-1, when added to the reaction mixture in a final concentration of 0.39 and 0.90 μg/ml, respectively. These experiments demonstrate that F6 not only inhibits the adsorption of HSV-1 to susceptible cells but also interferes with other biological properties of the virus in which gC is involved, supporting the hypothesis of an interaction between F6 and the viral glycoprotein. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09447113_v5_n3_p205_Pujol
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Glycoprotein
Hemagglutination inhibition
Herpes simplex virus
Natural antiviral
Rosette inhibition
Sulfated polysaccharide
algae
Herpes
Human herpesvirus 1
Nothogenia fastigiata
Ovis aries
Simplexvirus
spellingShingle Glycoprotein
Hemagglutination inhibition
Herpes simplex virus
Natural antiviral
Rosette inhibition
Sulfated polysaccharide
algae
Herpes
Human herpesvirus 1
Nothogenia fastigiata
Ovis aries
Simplexvirus
Pujol, C.A.
Matulewicz, M.C.
Cerezo, A.S.
Damonte, E.B.
Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus
topic_facet Glycoprotein
Hemagglutination inhibition
Herpes simplex virus
Natural antiviral
Rosette inhibition
Sulfated polysaccharide
algae
Herpes
Human herpesvirus 1
Nothogenia fastigiata
Ovis aries
Simplexvirus
description A purified sulfated xylomannan, named F6, obtained from the red seaweed Nothogenia fastigiata, proved to be a potent inhibitor of HSV-1 in vitro without affecting cell viability. In a virus yield reduction assay, the inhibitory concentration 50% (IC50) was 0.66 μg/ml. The mode of action of F6 was ascribed to an inhibitory effect on virus adsorption. The glycoprotein C (gC) of HSV-1 is involved in virus binding to the cell surface heparan sulfate. Furthermore, it mediates other biological activities such as induction of hemagglutination and binding to the third component of complement C3b. The compound F6 was effective in inhibiting hemagglutination induced by HSV-1 and also causes a reduction of 50% in rosette formation between sheep red blood cells coupled to C3b and cells infected with HSV-1, when added to the reaction mixture in a final concentration of 0.39 and 0.90 μg/ml, respectively. These experiments demonstrate that F6 not only inhibits the adsorption of HSV-1 to susceptible cells but also interferes with other biological properties of the virus in which gC is involved, supporting the hypothesis of an interaction between F6 and the viral glycoprotein.
format JOUR
author Pujol, C.A.
Matulewicz, M.C.
Cerezo, A.S.
Damonte, E.B.
author_facet Pujol, C.A.
Matulewicz, M.C.
Cerezo, A.S.
Damonte, E.B.
author_sort Pujol, C.A.
title Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus
title_short Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus
title_full Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus
title_fullStr Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus
title_full_unstemmed Inhibitory action of an algal derived xylomannan on glycoprotein C - Mediated biological properties of Herpes simplex virus
title_sort inhibitory action of an algal derived xylomannan on glycoprotein c - mediated biological properties of herpes simplex virus
url http://hdl.handle.net/20.500.12110/paper_09447113_v5_n3_p205_Pujol
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AT matulewiczmc inhibitoryactionofanalgalderivedxylomannanonglycoproteincmediatedbiologicalpropertiesofherpessimplexvirus
AT cerezoas inhibitoryactionofanalgalderivedxylomannanonglycoproteincmediatedbiologicalpropertiesofherpessimplexvirus
AT damonteeb inhibitoryactionofanalgalderivedxylomannanonglycoproteincmediatedbiologicalpropertiesofherpessimplexvirus
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