Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains
Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09445013_v211_n_p21_Sabate |
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todo:paper_09445013_v211_n_p21_Sabate |
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dspace |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacillus Biological control Lipopeptides Phaseolus vulgaris L. Sclerotinia sclerotiorum Bacilli Bacteriology Fungi Soils Antagonistic activity Bacterial contamination Biological controls Disease progression Germination potential Lipopeptides Phaseolus vulgaris l Sclerotinia Sclerotiorum Seed bacterium biocontrol agent biological control concentration (composition) fungicide fungus growth inoculation legume peptide pesticide application seedling siderophore Argentina Salta [Argentina] Bacilli (class) Bacillus (bacterium) Bacillus amyloliquefaciens Bacillus sp. Bacteria (microorganisms) Cladosporium Fungi Fusarium sp. Phaseolus vulgaris Rhizopus Sclerotinia sclerotiorum cyclopeptide fengycin fungicide indoleacetic acid derivative lipopeptide RNA 16S siderophore Ascomycetes Bacillus bacterium biological control agent chemistry classification disk diffusion drug effect Fabaceae genetics germination growth, development and aging isolation and purification metabolism microbiology phylogeny plant development plant disease plant seed prevention and control Ascomycota Bacillus Bacteria Biological Control Agents Disk Diffusion Antimicrobial Tests Fabaceae Fungicides, Industrial Germination Indoleacetic Acids Lipopeptides Peptides, Cyclic Phylogeny Plant Development Plant Diseases RNA, Ribosomal, 16S Seeds Siderophores |
| spellingShingle |
Bacillus Biological control Lipopeptides Phaseolus vulgaris L. Sclerotinia sclerotiorum Bacilli Bacteriology Fungi Soils Antagonistic activity Bacterial contamination Biological controls Disease progression Germination potential Lipopeptides Phaseolus vulgaris l Sclerotinia Sclerotiorum Seed bacterium biocontrol agent biological control concentration (composition) fungicide fungus growth inoculation legume peptide pesticide application seedling siderophore Argentina Salta [Argentina] Bacilli (class) Bacillus (bacterium) Bacillus amyloliquefaciens Bacillus sp. Bacteria (microorganisms) Cladosporium Fungi Fusarium sp. Phaseolus vulgaris Rhizopus Sclerotinia sclerotiorum cyclopeptide fengycin fungicide indoleacetic acid derivative lipopeptide RNA 16S siderophore Ascomycetes Bacillus bacterium biological control agent chemistry classification disk diffusion drug effect Fabaceae genetics germination growth, development and aging isolation and purification metabolism microbiology phylogeny plant development plant disease plant seed prevention and control Ascomycota Bacillus Bacteria Biological Control Agents Disk Diffusion Antimicrobial Tests Fabaceae Fungicides, Industrial Germination Indoleacetic Acids Lipopeptides Peptides, Cyclic Phylogeny Plant Development Plant Diseases RNA, Ribosomal, 16S Seeds Siderophores Sabaté, D.C. Brandan, C.P. Petroselli, G. Erra-Balsells, R. Audisio, M.C. Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| topic_facet |
Bacillus Biological control Lipopeptides Phaseolus vulgaris L. Sclerotinia sclerotiorum Bacilli Bacteriology Fungi Soils Antagonistic activity Bacterial contamination Biological controls Disease progression Germination potential Lipopeptides Phaseolus vulgaris l Sclerotinia Sclerotiorum Seed bacterium biocontrol agent biological control concentration (composition) fungicide fungus growth inoculation legume peptide pesticide application seedling siderophore Argentina Salta [Argentina] Bacilli (class) Bacillus (bacterium) Bacillus amyloliquefaciens Bacillus sp. Bacteria (microorganisms) Cladosporium Fungi Fusarium sp. Phaseolus vulgaris Rhizopus Sclerotinia sclerotiorum cyclopeptide fengycin fungicide indoleacetic acid derivative lipopeptide RNA 16S siderophore Ascomycetes Bacillus bacterium biological control agent chemistry classification disk diffusion drug effect Fabaceae genetics germination growth, development and aging isolation and purification metabolism microbiology phylogeny plant development plant disease plant seed prevention and control Ascomycota Bacillus Bacteria Biological Control Agents Disk Diffusion Antimicrobial Tests Fabaceae Fungicides, Industrial Germination Indoleacetic Acids Lipopeptides Peptides, Cyclic Phylogeny Plant Development Plant Diseases RNA, Ribosomal, 16S Seeds Siderophores |
| description |
Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased crop germination potential (GP) from the common bean by 14.5% compared to control seeds; these strains also increased root length (10.4 and 15%, respectively) and stem length (20.2 and 30%, respectively) compared to the control; however, as for the B14 strain, no increases in growth parameters were detected. In addition, all the treatments that combined two bacilli: B14 + B19, B14 + P12 and B19 + P12, generated beneficial effects on GP and seedling growth compared to control seeds, but not compared to a single inoculant. B19 and P12 strains synthesized auxins at concentrations of 5.71 and 4.90 mg/mL, respectively, and it was qualitatively determined that they synthesize siderophores. In addition, previous studies have determined that B14 produces auxins in a concentration of 10.10 mg/mL, and qualitatively synthesizes siderophores. The phytosanitary state of the white bean cv. Alubia control seeds revealed bacterial contamination in 87% of all the evaluated seeds and different fungi such as Cladosporium sp., Fusarium sp., and Rhizopus sp. Bean seeds treated with B14, B19 or P12 showed no growth of contaminating bacteria or of pathogenic fungi; in fact, bacilli inoculum development was observed in all seeds. Additionally, B19, P12 and B14 strains inhibited in vitro the development of 9 native S. sclerotiorum strains isolated from the Salta region, with FI ranging between 60 and 100%. The three Bacillus strains synthesized different isoforms of the lipopeptides: surfactin, iturin, and fengycin in the presence of S. sclerotiorum, as determined by MALDI-TOF. In the in vivo trials, when common bean seeds were grown in soils contaminated with S. sclerotiorum, an incidence of 100% was determined when the seeds were not treated with any Bacillus. Seeds treated with the chemical fungicide and sown in S. sclerotiorum-infested soil did not produce seed emergence, while the inoculation of the seeds with B14 + P12, B14 + B19 or B19 + P12 reduced the effect of the pathogen by 46, 43 and 25%, respectively. Disease progression in B14 + P12 and B14 + B19 treatments was significantly lower than in the remaining treatments, with an AUDPC of 873.75 and 1071, respectively. © 2018 Elsevier GmbH |
| format |
JOUR |
| author |
Sabaté, D.C. Brandan, C.P. Petroselli, G. Erra-Balsells, R. Audisio, M.C. |
| author_facet |
Sabaté, D.C. Brandan, C.P. Petroselli, G. Erra-Balsells, R. Audisio, M.C. |
| author_sort |
Sabaté, D.C. |
| title |
Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_short |
Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_full |
Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_fullStr |
Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_full_unstemmed |
Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_sort |
biocontrol of sclerotinia sclerotiorum (lib.) de bary on common bean by native lipopeptide-producer bacillus strains |
| url |
http://hdl.handle.net/20.500.12110/paper_09445013_v211_n_p21_Sabate |
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AT sabatedc biocontrolofsclerotiniasclerotiorumlibdebaryoncommonbeanbynativelipopeptideproducerbacillusstrains AT brandancp biocontrolofsclerotiniasclerotiorumlibdebaryoncommonbeanbynativelipopeptideproducerbacillusstrains AT petrosellig biocontrolofsclerotiniasclerotiorumlibdebaryoncommonbeanbynativelipopeptideproducerbacillusstrains AT errabalsellsr biocontrolofsclerotiniasclerotiorumlibdebaryoncommonbeanbynativelipopeptideproducerbacillusstrains AT audisiomc biocontrolofsclerotiniasclerotiorumlibdebaryoncommonbeanbynativelipopeptideproducerbacillusstrains |
| _version_ |
1807323659442847744 |
| spelling |
todo:paper_09445013_v211_n_p21_Sabate2023-10-03T15:49:15Z Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains Sabaté, D.C. Brandan, C.P. Petroselli, G. Erra-Balsells, R. Audisio, M.C. Bacillus Biological control Lipopeptides Phaseolus vulgaris L. Sclerotinia sclerotiorum Bacilli Bacteriology Fungi Soils Antagonistic activity Bacterial contamination Biological controls Disease progression Germination potential Lipopeptides Phaseolus vulgaris l Sclerotinia Sclerotiorum Seed bacterium biocontrol agent biological control concentration (composition) fungicide fungus growth inoculation legume peptide pesticide application seedling siderophore Argentina Salta [Argentina] Bacilli (class) Bacillus (bacterium) Bacillus amyloliquefaciens Bacillus sp. Bacteria (microorganisms) Cladosporium Fungi Fusarium sp. Phaseolus vulgaris Rhizopus Sclerotinia sclerotiorum cyclopeptide fengycin fungicide indoleacetic acid derivative lipopeptide RNA 16S siderophore Ascomycetes Bacillus bacterium biological control agent chemistry classification disk diffusion drug effect Fabaceae genetics germination growth, development and aging isolation and purification metabolism microbiology phylogeny plant development plant disease plant seed prevention and control Ascomycota Bacillus Bacteria Biological Control Agents Disk Diffusion Antimicrobial Tests Fabaceae Fungicides, Industrial Germination Indoleacetic Acids Lipopeptides Peptides, Cyclic Phylogeny Plant Development Plant Diseases RNA, Ribosomal, 16S Seeds Siderophores Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased crop germination potential (GP) from the common bean by 14.5% compared to control seeds; these strains also increased root length (10.4 and 15%, respectively) and stem length (20.2 and 30%, respectively) compared to the control; however, as for the B14 strain, no increases in growth parameters were detected. In addition, all the treatments that combined two bacilli: B14 + B19, B14 + P12 and B19 + P12, generated beneficial effects on GP and seedling growth compared to control seeds, but not compared to a single inoculant. B19 and P12 strains synthesized auxins at concentrations of 5.71 and 4.90 mg/mL, respectively, and it was qualitatively determined that they synthesize siderophores. In addition, previous studies have determined that B14 produces auxins in a concentration of 10.10 mg/mL, and qualitatively synthesizes siderophores. The phytosanitary state of the white bean cv. Alubia control seeds revealed bacterial contamination in 87% of all the evaluated seeds and different fungi such as Cladosporium sp., Fusarium sp., and Rhizopus sp. Bean seeds treated with B14, B19 or P12 showed no growth of contaminating bacteria or of pathogenic fungi; in fact, bacilli inoculum development was observed in all seeds. Additionally, B19, P12 and B14 strains inhibited in vitro the development of 9 native S. sclerotiorum strains isolated from the Salta region, with FI ranging between 60 and 100%. The three Bacillus strains synthesized different isoforms of the lipopeptides: surfactin, iturin, and fengycin in the presence of S. sclerotiorum, as determined by MALDI-TOF. In the in vivo trials, when common bean seeds were grown in soils contaminated with S. sclerotiorum, an incidence of 100% was determined when the seeds were not treated with any Bacillus. Seeds treated with the chemical fungicide and sown in S. sclerotiorum-infested soil did not produce seed emergence, while the inoculation of the seeds with B14 + P12, B14 + B19 or B19 + P12 reduced the effect of the pathogen by 46, 43 and 25%, respectively. Disease progression in B14 + P12 and B14 + B19 treatments was significantly lower than in the remaining treatments, with an AUDPC of 873.75 and 1071, respectively. © 2018 Elsevier GmbH JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09445013_v211_n_p21_Sabate |