Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine
Little attention has been paid to the impact of wastewater generated by mining activities on fluoride. In this study, we evaluated the hematology responses of common South American toad Rhinella arenarum inhabiting natural and artificial environments associated with a fluorite mine from central Arge...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | INPR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09441344_v_n_p1_Pollo |
| Aporte de: |
| id |
todo:paper_09441344_v_n_p1_Pollo |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_09441344_v_n_p1_Pollo2023-10-03T15:49:09Z Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine Pollo, F.E. Grenat, P.R. Salinas, Z.A. Otero, M.A. Salas, N.E. Martino, A.L. Fluorite mine Leukocyte Micronuclei Nuclear abnormalities Rhinella arenarum Little attention has been paid to the impact of wastewater generated by mining activities on fluoride. In this study, we evaluated the hematology responses of common South American toad Rhinella arenarum inhabiting natural and artificial environments associated with a fluorite mine from central Argentina. We analyzed three sampling stations associated with the fluorite mine: (I) Los Cerros Negros stream (CN), which runs on granitic rock with a high fluorite content; (II) Los Vallecitos stream (LV), which runs on metamorphic rock with low fluorite content; and (III) artificial decantation ponds (DP) containing sediments produced by fluorite flotation process. We calculated frequencies of micronuclei, erythrocyte nuclear abnormalities, mitosis, and immature erythrocytes. In addition, we performed a differential leukocyte count and determined neutrophils/lymphocyte ratio as a stress response estimator. We found high micronucleus (MN) and erythrocyte nuclear abnormality (ENA) frequencies in DP and CN but low frequencies in LV. The neutrophil/lymphocyte ratio was different among sites, with a significant increase in individuals from DP. Values registered in DP could be caused by exposure to mixture of compounds registered in dams that hold wastewater, while high values registered in CN stream might be due to natural concentrations of fluoride. Our results suggest that blood is an effective and non-destructive sensitive indicator for monitoring genotoxic agents in freshwater ecosystems. © 2017 Springer-Verlag GmbH Germany INPR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09441344_v_n_p1_Pollo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fluorite mine Leukocyte Micronuclei Nuclear abnormalities Rhinella arenarum |
| spellingShingle |
Fluorite mine Leukocyte Micronuclei Nuclear abnormalities Rhinella arenarum Pollo, F.E. Grenat, P.R. Salinas, Z.A. Otero, M.A. Salas, N.E. Martino, A.L. Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine |
| topic_facet |
Fluorite mine Leukocyte Micronuclei Nuclear abnormalities Rhinella arenarum |
| description |
Little attention has been paid to the impact of wastewater generated by mining activities on fluoride. In this study, we evaluated the hematology responses of common South American toad Rhinella arenarum inhabiting natural and artificial environments associated with a fluorite mine from central Argentina. We analyzed three sampling stations associated with the fluorite mine: (I) Los Cerros Negros stream (CN), which runs on granitic rock with a high fluorite content; (II) Los Vallecitos stream (LV), which runs on metamorphic rock with low fluorite content; and (III) artificial decantation ponds (DP) containing sediments produced by fluorite flotation process. We calculated frequencies of micronuclei, erythrocyte nuclear abnormalities, mitosis, and immature erythrocytes. In addition, we performed a differential leukocyte count and determined neutrophils/lymphocyte ratio as a stress response estimator. We found high micronucleus (MN) and erythrocyte nuclear abnormality (ENA) frequencies in DP and CN but low frequencies in LV. The neutrophil/lymphocyte ratio was different among sites, with a significant increase in individuals from DP. Values registered in DP could be caused by exposure to mixture of compounds registered in dams that hold wastewater, while high values registered in CN stream might be due to natural concentrations of fluoride. Our results suggest that blood is an effective and non-destructive sensitive indicator for monitoring genotoxic agents in freshwater ecosystems. © 2017 Springer-Verlag GmbH Germany |
| format |
INPR |
| author |
Pollo, F.E. Grenat, P.R. Salinas, Z.A. Otero, M.A. Salas, N.E. Martino, A.L. |
| author_facet |
Pollo, F.E. Grenat, P.R. Salinas, Z.A. Otero, M.A. Salas, N.E. Martino, A.L. |
| author_sort |
Pollo, F.E. |
| title |
Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine |
| title_short |
Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine |
| title_full |
Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine |
| title_fullStr |
Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine |
| title_full_unstemmed |
Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine |
| title_sort |
evaluation in situ of genotoxicity and stress in south american common toad rhinella arenarum in environments related to fluorite mine |
| url |
http://hdl.handle.net/20.500.12110/paper_09441344_v_n_p1_Pollo |
| work_keys_str_mv |
AT pollofe evaluationinsituofgenotoxicityandstressinsouthamericancommontoadrhinellaarenaruminenvironmentsrelatedtofluoritemine AT grenatpr evaluationinsituofgenotoxicityandstressinsouthamericancommontoadrhinellaarenaruminenvironmentsrelatedtofluoritemine AT salinasza evaluationinsituofgenotoxicityandstressinsouthamericancommontoadrhinellaarenaruminenvironmentsrelatedtofluoritemine AT oteroma evaluationinsituofgenotoxicityandstressinsouthamericancommontoadrhinellaarenaruminenvironmentsrelatedtofluoritemine AT salasne evaluationinsituofgenotoxicityandstressinsouthamericancommontoadrhinellaarenaruminenvironmentsrelatedtofluoritemine AT martinoal evaluationinsituofgenotoxicityandstressinsouthamericancommontoadrhinellaarenaruminenvironmentsrelatedtofluoritemine |
| _version_ |
1807320597093416960 |