Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis

A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins wa...

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Detalles Bibliográficos
Autores principales: Rossetti, M.V., Juknat, A.A., del Batlle, A.M.C.
Formato: JOUR
Materias:
Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09395075_v44_n7-8_p578_Rossetti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09395075_v44_n7-8_p578_Rossetti
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spelling todo:paper_09395075_v44_n7-8_p578_Rossetti2023-10-03T15:48:49Z Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis Rossetti, M.V. Juknat, A.A. del Batlle, A.M.C. Euglena gracilis Particulate Porphobilinogen-Deaminase Porphyrins Soluble A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deaminase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000. © 1989, Verlag der Zeitschrift. All rights reserved. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Batlle, A.M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09395075_v44_n7-8_p578_Rossetti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
spellingShingle Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
Rossetti, M.V.
Juknat, A.A.
del Batlle, A.M.C.
Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
topic_facet Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
description A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deaminase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000. © 1989, Verlag der Zeitschrift. All rights reserved.
format JOUR
author Rossetti, M.V.
Juknat, A.A.
del Batlle, A.M.C.
author_facet Rossetti, M.V.
Juknat, A.A.
del Batlle, A.M.C.
author_sort Rossetti, M.V.
title Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_short Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_full Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_fullStr Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_full_unstemmed Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_sort soluble and particulate porphobilinogen-deaminase from dark-grown euglena gracilis
url http://hdl.handle.net/20.500.12110/paper_09395075_v44_n7-8_p578_Rossetti
work_keys_str_mv AT rossettimv solubleandparticulateporphobilinogendeaminasefromdarkgrowneuglenagracilis
AT juknataa solubleandparticulateporphobilinogendeaminasefromdarkgrowneuglenagracilis
AT delbatlleamc solubleandparticulateporphobilinogendeaminasefromdarkgrowneuglenagracilis
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