Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z.

Lysed cells as well as different fractions of isolated, disrupted chloroplasts from light grown Euglena gracilis were tested for their capability of porphyrin biosynthesis. It is shown that the formation of the soluble porphobilinogenase (PBG-ase) fraction is inhibited by cycloheximide indicating it...

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Detalles Bibliográficos
Autores principales: Juknat, A.A., Dörnemann, D., Senger, H.
Formato: JOUR
Materias:
Euglena Chloroplasts
Euglena gracilis Z
Porphobilinogenase
Porphyrin Biosynthesis
Porphyrinogens
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09395075_v44_n1-2_p85_Juknat
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09395075_v44_n1-2_p85_Juknat
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spelling todo:paper_09395075_v44_n1-2_p85_Juknat2023-10-03T15:48:49Z Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z. Juknat, A.A. Dörnemann, D. Senger, H. Euglena Chloroplasts Euglena gracilis Z Porphobilinogenase Porphyrin Biosynthesis Porphyrinogens Lysed cells as well as different fractions of isolated, disrupted chloroplasts from light grown Euglena gracilis were tested for their capability of porphyrin biosynthesis. It is shown that the formation of the soluble porphobilinogenase (PBG-ase) fraction is inhibited by cycloheximide indicating its biosyntheses on 80S ribosomes, whereas the formation of the membrane bound chloroplast PBG-ase is chloramphenicol sensitive. Different pH-optima are demonstrated for the two enzymes. From the presented results it is deduced that PBG-ase from chloroplasts is different from those isolated from dark grown cells. © 1989, Verlag der Zeitschrift für Naturforschung. All rights reserved. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09395075_v44_n1-2_p85_Juknat
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Euglena Chloroplasts
Euglena gracilis Z
Porphobilinogenase
Porphyrin Biosynthesis
Porphyrinogens
spellingShingle Euglena Chloroplasts
Euglena gracilis Z
Porphobilinogenase
Porphyrin Biosynthesis
Porphyrinogens
Juknat, A.A.
Dörnemann, D.
Senger, H.
Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z.
topic_facet Euglena Chloroplasts
Euglena gracilis Z
Porphobilinogenase
Porphyrin Biosynthesis
Porphyrinogens
description Lysed cells as well as different fractions of isolated, disrupted chloroplasts from light grown Euglena gracilis were tested for their capability of porphyrin biosynthesis. It is shown that the formation of the soluble porphobilinogenase (PBG-ase) fraction is inhibited by cycloheximide indicating its biosyntheses on 80S ribosomes, whereas the formation of the membrane bound chloroplast PBG-ase is chloramphenicol sensitive. Different pH-optima are demonstrated for the two enzymes. From the presented results it is deduced that PBG-ase from chloroplasts is different from those isolated from dark grown cells. © 1989, Verlag der Zeitschrift für Naturforschung. All rights reserved.
format JOUR
author Juknat, A.A.
Dörnemann, D.
Senger, H.
author_facet Juknat, A.A.
Dörnemann, D.
Senger, H.
author_sort Juknat, A.A.
title Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z.
title_short Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z.
title_full Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z.
title_fullStr Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z.
title_full_unstemmed Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z.
title_sort different porphobilinogenases in cytoplasm and isolated chloroplasts from light-grown euglena gracilis z.
url http://hdl.handle.net/20.500.12110/paper_09395075_v44_n1-2_p85_Juknat
work_keys_str_mv AT juknataa differentporphobilinogenasesincytoplasmandisolatedchloroplastsfromlightgrowneuglenagracilisz
AT dornemannd differentporphobilinogenasesincytoplasmandisolatedchloroplastsfromlightgrowneuglenagracilisz
AT sengerh differentporphobilinogenasesincytoplasmandisolatedchloroplastsfromlightgrowneuglenagracilisz
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