Lysine transporters in human trypanosomatid pathogens
In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these par...
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todo:paper_09394451_v42_n1_p347_Inbar2023-10-03T15:48:46Z Lysine transporters in human trypanosomatid pathogens Inbar, E. Canepa, G.E. Carrillo, C. Glaser, F. Grotemeyer, M.S. Rentsch, D. Zilberstein, D. Pereira, C.A. Amino acid transport Leishmania Lysine transport Trypanosoma alanine amino acid transporter arginine asparagine aspartic acid auxin cysteine glycine histidine Leishmania donovani AAP7 lysine lysine transporter methionine permease proline serine threonine Trypanosoma cruzi AAP7 unclassified drug conference paper enzyme kinetics enzyme regulation enzyme specificity epimastigote human immunofluorescence test Leishmania donovani life cycle nucleotide sequence open reading frame parasite survival phylogeny priority journal promastigote protein expression protein localization Saccharomyces cerevisiae sequence analysis Trypanosoma Trypanosoma cruzi trypanosomatid infection Eukaryota Leishmania donovani Naso hexacanthus Trypanosoma Trypanosoma cruzi Trypanosomatidae In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these parasite transporters translocate only arginine. This phenomenon prompted us to identify and characterize parasite lysine transporters. Here we demonstrate that LdAAP7 and TcAAP7 encode lysine-specific permeases in L. donovani and T. cruzi, respectively. These two lysine permeases are both members of the large amino acid/auxin permease family and share certain biochemical properties, such as specificity and Km. However, we evidence that LdAAP7 and TcAAP7 differ in their regulation and localization, such differences are likely a reflection of the dissimilar L. donovani and T. cruzi life cycles. Failed attempts to delete both alleles of LdAAP7 support the premise that this is an essential gene that encodes the only lysine permeases expressed in L. donovani promastigotes and T. cruzi epimastigotes, respectively. © Springer-Verlag 2010. Fil:Canepa, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carrillo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereira, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09394451_v42_n1_p347_Inbar |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amino acid transport Leishmania Lysine transport Trypanosoma alanine amino acid transporter arginine asparagine aspartic acid auxin cysteine glycine histidine Leishmania donovani AAP7 lysine lysine transporter methionine permease proline serine threonine Trypanosoma cruzi AAP7 unclassified drug conference paper enzyme kinetics enzyme regulation enzyme specificity epimastigote human immunofluorescence test Leishmania donovani life cycle nucleotide sequence open reading frame parasite survival phylogeny priority journal promastigote protein expression protein localization Saccharomyces cerevisiae sequence analysis Trypanosoma Trypanosoma cruzi trypanosomatid infection Eukaryota Leishmania donovani Naso hexacanthus Trypanosoma Trypanosoma cruzi Trypanosomatidae |
| spellingShingle |
Amino acid transport Leishmania Lysine transport Trypanosoma alanine amino acid transporter arginine asparagine aspartic acid auxin cysteine glycine histidine Leishmania donovani AAP7 lysine lysine transporter methionine permease proline serine threonine Trypanosoma cruzi AAP7 unclassified drug conference paper enzyme kinetics enzyme regulation enzyme specificity epimastigote human immunofluorescence test Leishmania donovani life cycle nucleotide sequence open reading frame parasite survival phylogeny priority journal promastigote protein expression protein localization Saccharomyces cerevisiae sequence analysis Trypanosoma Trypanosoma cruzi trypanosomatid infection Eukaryota Leishmania donovani Naso hexacanthus Trypanosoma Trypanosoma cruzi Trypanosomatidae Inbar, E. Canepa, G.E. Carrillo, C. Glaser, F. Grotemeyer, M.S. Rentsch, D. Zilberstein, D. Pereira, C.A. Lysine transporters in human trypanosomatid pathogens |
| topic_facet |
Amino acid transport Leishmania Lysine transport Trypanosoma alanine amino acid transporter arginine asparagine aspartic acid auxin cysteine glycine histidine Leishmania donovani AAP7 lysine lysine transporter methionine permease proline serine threonine Trypanosoma cruzi AAP7 unclassified drug conference paper enzyme kinetics enzyme regulation enzyme specificity epimastigote human immunofluorescence test Leishmania donovani life cycle nucleotide sequence open reading frame parasite survival phylogeny priority journal promastigote protein expression protein localization Saccharomyces cerevisiae sequence analysis Trypanosoma Trypanosoma cruzi trypanosomatid infection Eukaryota Leishmania donovani Naso hexacanthus Trypanosoma Trypanosoma cruzi Trypanosomatidae |
| description |
In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these parasite transporters translocate only arginine. This phenomenon prompted us to identify and characterize parasite lysine transporters. Here we demonstrate that LdAAP7 and TcAAP7 encode lysine-specific permeases in L. donovani and T. cruzi, respectively. These two lysine permeases are both members of the large amino acid/auxin permease family and share certain biochemical properties, such as specificity and Km. However, we evidence that LdAAP7 and TcAAP7 differ in their regulation and localization, such differences are likely a reflection of the dissimilar L. donovani and T. cruzi life cycles. Failed attempts to delete both alleles of LdAAP7 support the premise that this is an essential gene that encodes the only lysine permeases expressed in L. donovani promastigotes and T. cruzi epimastigotes, respectively. © Springer-Verlag 2010. |
| format |
JOUR |
| author |
Inbar, E. Canepa, G.E. Carrillo, C. Glaser, F. Grotemeyer, M.S. Rentsch, D. Zilberstein, D. Pereira, C.A. |
| author_facet |
Inbar, E. Canepa, G.E. Carrillo, C. Glaser, F. Grotemeyer, M.S. Rentsch, D. Zilberstein, D. Pereira, C.A. |
| author_sort |
Inbar, E. |
| title |
Lysine transporters in human trypanosomatid pathogens |
| title_short |
Lysine transporters in human trypanosomatid pathogens |
| title_full |
Lysine transporters in human trypanosomatid pathogens |
| title_fullStr |
Lysine transporters in human trypanosomatid pathogens |
| title_full_unstemmed |
Lysine transporters in human trypanosomatid pathogens |
| title_sort |
lysine transporters in human trypanosomatid pathogens |
| url |
http://hdl.handle.net/20.500.12110/paper_09394451_v42_n1_p347_Inbar |
| work_keys_str_mv |
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1807317642284892160 |