Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta

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The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regar...

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Autores principales:	Diessler, M., Ventureira, M., Hernandez, R., Sobarzo, C., Casas, L., Barbeito, C., Cebral, E.
Formato:	JOUR
Materias:	gelatinase A gelatinase B animal dog endometrium female immunohistochemistry metabolism placenta pregnancy reproduction Animals Dogs Endometrium Female Immunohistochemistry Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Placenta Placentation Pregnancy
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09366768_v52_n1_p35_Diessler
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09366768_v52_n1_p35_Diessler
record_format	dspace
spelling	todo:paper_09366768_v52_n1_p35_Diessler2023-10-03T15:48:42Z Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta Diessler, M. Ventureira, M. Hernandez, R. Sobarzo, C. Casas, L. Barbeito, C. Cebral, E. gelatinase A gelatinase B animal dog endometrium female immunohistochemistry metabolism placenta pregnancy reproduction Animals Dogs Endometrium Female Immunohistochemistry Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Placenta Placentation Pregnancy The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the expression of MMP enzymes has been studied in the cat and the mature canine placenta. The aim of this study was to analyse the expression and activity of MMP-2 and MMP-9 in the early dog placenta. Placentae from 18 to 30 days of pregnancy were collected from four bitches. Two placentae from each bitch were analysed. Placental tissue from one uterine horn was fixed in formaldehyde for immunohistochemistry, while marginal haematoma, labyrinth, non-implantative and implantative endometrium from the contralateral horn were immediately frozen in dry ice for the analysis of MMP expression (Western blot [WB]) and activity (zymography). MMP-2 and MMP-9 were evidenced in the labyrinth, maternal glands and marginal haematoma; this finding was directly correlated with levels of MMP expression by WB, and with the activity of MMP-2, mainly in the haematoma (the area of major remodelling of tissues). Thus, although MMP-9 is well expressed in the early canine placenta, it is not active. Given the important role of MMPs for invasiveness, maternal–foetal angiogenesis and the establishment of a correct foetal nutrition, the results are consistent with the findings in other species in which the MMP-2 activation precedes the MMP-9 one in early placentation. © 2016 Blackwell Verlag GmbH Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09366768_v52_n1_p35_Diessler
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	gelatinase A gelatinase B animal dog endometrium female immunohistochemistry metabolism placenta pregnancy reproduction Animals Dogs Endometrium Female Immunohistochemistry Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Placenta Placentation Pregnancy
spellingShingle	gelatinase A gelatinase B animal dog endometrium female immunohistochemistry metabolism placenta pregnancy reproduction Animals Dogs Endometrium Female Immunohistochemistry Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Placenta Placentation Pregnancy Diessler, M. Ventureira, M. Hernandez, R. Sobarzo, C. Casas, L. Barbeito, C. Cebral, E. Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
topic_facet	gelatinase A gelatinase B animal dog endometrium female immunohistochemistry metabolism placenta pregnancy reproduction Animals Dogs Endometrium Female Immunohistochemistry Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Placenta Placentation Pregnancy
description	The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the expression of MMP enzymes has been studied in the cat and the mature canine placenta. The aim of this study was to analyse the expression and activity of MMP-2 and MMP-9 in the early dog placenta. Placentae from 18 to 30 days of pregnancy were collected from four bitches. Two placentae from each bitch were analysed. Placental tissue from one uterine horn was fixed in formaldehyde for immunohistochemistry, while marginal haematoma, labyrinth, non-implantative and implantative endometrium from the contralateral horn were immediately frozen in dry ice for the analysis of MMP expression (Western blot [WB]) and activity (zymography). MMP-2 and MMP-9 were evidenced in the labyrinth, maternal glands and marginal haematoma; this finding was directly correlated with levels of MMP expression by WB, and with the activity of MMP-2, mainly in the haematoma (the area of major remodelling of tissues). Thus, although MMP-9 is well expressed in the early canine placenta, it is not active. Given the important role of MMPs for invasiveness, maternal–foetal angiogenesis and the establishment of a correct foetal nutrition, the results are consistent with the findings in other species in which the MMP-2 activation precedes the MMP-9 one in early placentation. © 2016 Blackwell Verlag GmbH
format	JOUR
author	Diessler, M. Ventureira, M. Hernandez, R. Sobarzo, C. Casas, L. Barbeito, C. Cebral, E.
author_facet	Diessler, M. Ventureira, M. Hernandez, R. Sobarzo, C. Casas, L. Barbeito, C. Cebral, E.
author_sort	Diessler, M.
title	Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_short	Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_full	Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_fullStr	Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_full_unstemmed	Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_sort	differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
url	http://hdl.handle.net/20.500.12110/paper_09366768_v52_n1_p35_Diessler
work_keys_str_mv	AT diesslerm differentialexpressionandactivityofmatrixmetalloproteinases2and9incanineearlyplacenta AT ventureiram differentialexpressionandactivityofmatrixmetalloproteinases2and9incanineearlyplacenta AT hernandezr differentialexpressionandactivityofmatrixmetalloproteinases2and9incanineearlyplacenta AT sobarzoc differentialexpressionandactivityofmatrixmetalloproteinases2and9incanineearlyplacenta AT casasl differentialexpressionandactivityofmatrixmetalloproteinases2and9incanineearlyplacenta AT barbeitoc differentialexpressionandactivityofmatrixmetalloproteinases2and9incanineearlyplacenta AT cebrale differentialexpressionandactivityofmatrixmetalloproteinases2and9incanineearlyplacenta
_version_	1807321614625275904

Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta

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