Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers

The development of dry rot caused by Fusarium solani f. sp. eumartii was evaluated in susceptible (Huinkul) and resistant (Spunta) potato cultivars. Fungal proteolytic and polygalacturanase activities were measured at different days postinoculation either with the pathogenic F. solani f. sp. eumarti...

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Autores principales: Olivieri, F.P., Maldonado, S., Tonón, C.V., Casalongué, C.A.
Formato: JOUR
Materias:
Cell wall degrading enzymes
Fungal proteases
Fusarium solani
Solanum tuberosum
disease resistance
enzyme activity
infectious disease
inoculation
tuber
Fungi
Fusarium
Fusarium sp.
Solanum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09311785_v152_n6_p337_Olivieri
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_09311785_v152_n6_p337_Olivieri2023-10-03T15:48:10Z Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers Olivieri, F.P. Maldonado, S. Tonón, C.V. Casalongué, C.A. Cell wall degrading enzymes Fungal proteases Fusarium solani Solanum tuberosum disease resistance enzyme activity infectious disease inoculation tuber Fungi Fusarium Fusarium solani Fusarium sp. Solanum Solanum tuberosum The development of dry rot caused by Fusarium solani f. sp. eumartii was evaluated in susceptible (Huinkul) and resistant (Spunta) potato cultivars. Fungal proteolytic and polygalacturanase activities were measured at different days postinoculation either with the pathogenic F. solani f. sp. eumartii, isolate 3122 or with the non-pathogenic F. solani, isolate 1042. After inoculation with the pathogenic fungus, proteolytic and polygalaturonase activities were higher in the susceptible than in the resistant cultivar. In addition, we found a correlation between the levels of proteolytic activity detected in the intercellular washing fluids with the size of the lesion area caused by F. solani f. sp. eumartii in Huinkul tubers. The action of the proteolytic activity over cell wall proteins of both potato cultivars was assayed. An extracellular potato protein with homology to proteinase inhibitors of the Kunitz family was identified as a substrate of the proteolytic activity in the susceptible cultivar. A microscopic study revealed differences between the potato genotypes in the rate of response to infection by F. solani f. sp. eumartii. In addition, the cell wall alteration caused by F. solani f. sp. eumartii in cortical cells of susceptible tubers was evaluated. The data with respect to the correlation between the course of cyto- and biochemical events of the two host-pathogen interactions were discussed. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09311785_v152_n6_p337_Olivieri
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cell wall degrading enzymes
Fungal proteases
Fusarium solani
Solanum tuberosum
disease resistance
enzyme activity
infectious disease
inoculation
tuber
Fungi
Fusarium
Fusarium solani
Fusarium sp.
Solanum
Solanum tuberosum
spellingShingle Cell wall degrading enzymes
Fungal proteases
Fusarium solani
Solanum tuberosum
disease resistance
enzyme activity
infectious disease
inoculation
tuber
Fungi
Fusarium
Fusarium solani
Fusarium sp.
Solanum
Solanum tuberosum
Olivieri, F.P.
Maldonado, S.
Tonón, C.V.
Casalongué, C.A.
Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers
topic_facet Cell wall degrading enzymes
Fungal proteases
Fusarium solani
Solanum tuberosum
disease resistance
enzyme activity
infectious disease
inoculation
tuber
Fungi
Fusarium
Fusarium solani
Fusarium sp.
Solanum
Solanum tuberosum
description The development of dry rot caused by Fusarium solani f. sp. eumartii was evaluated in susceptible (Huinkul) and resistant (Spunta) potato cultivars. Fungal proteolytic and polygalacturanase activities were measured at different days postinoculation either with the pathogenic F. solani f. sp. eumartii, isolate 3122 or with the non-pathogenic F. solani, isolate 1042. After inoculation with the pathogenic fungus, proteolytic and polygalaturonase activities were higher in the susceptible than in the resistant cultivar. In addition, we found a correlation between the levels of proteolytic activity detected in the intercellular washing fluids with the size of the lesion area caused by F. solani f. sp. eumartii in Huinkul tubers. The action of the proteolytic activity over cell wall proteins of both potato cultivars was assayed. An extracellular potato protein with homology to proteinase inhibitors of the Kunitz family was identified as a substrate of the proteolytic activity in the susceptible cultivar. A microscopic study revealed differences between the potato genotypes in the rate of response to infection by F. solani f. sp. eumartii. In addition, the cell wall alteration caused by F. solani f. sp. eumartii in cortical cells of susceptible tubers was evaluated. The data with respect to the correlation between the course of cyto- and biochemical events of the two host-pathogen interactions were discussed.
format JOUR
author Olivieri, F.P.
Maldonado, S.
Tonón, C.V.
Casalongué, C.A.
author_facet Olivieri, F.P.
Maldonado, S.
Tonón, C.V.
Casalongué, C.A.
author_sort Olivieri, F.P.
title Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers
title_short Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers
title_full Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers
title_fullStr Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers
title_full_unstemmed Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers
title_sort hydrolytic activities of fusarium solani and fusarium solani f. sp. eumartii associated with the infection process of potato tubers
url http://hdl.handle.net/20.500.12110/paper_09311785_v152_n6_p337_Olivieri
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AT tononcv hydrolyticactivitiesoffusariumsolaniandfusariumsolanifspeumartiiassociatedwiththeinfectionprocessofpotatotubers
AT casalongueca hydrolyticactivitiesoffusariumsolaniandfusariumsolanifspeumartiiassociatedwiththeinfectionprocessofpotatotubers
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