PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition

The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addit...

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Autores principales:	Fernández, M.R., Casabona, M.G., Anupama, V.N., Krishnakumar, B., Curutchet, G.A., Bernik, D.L.
Formato:	JOUR
Materias:	Bed material Biofilm CSLM PDMS SEM Acidithiobacillus ferrooxidans Bacterial biofilm Bacterial viability Bed materials Bed particles Biofilm formation Confocal laser scanning Hydrophilic groups Hydrophilic polymers Interconnected porous structure Particle surface Polydimethylsiloxane PDMS Polymer composition Porous particle Sulphate-reducing bacteria Surface adsorption Tannery effluent Tetraethoxysilanes Xanthan Gum Adhesion Adsorption Bacteriology Biofilms Biofilters Cell adhesion Cell immobilization Fluorescence microscopy Hydrophilicity Microchannels Polymers Pore size Porous materials Scanning electron microscopy Silicones Surfaces dimeticone tetraethoxysilane xanthan article bacterial membrane bacterial strain bacterial viability bacterium adherence bacterium culture biofilm controlled study hydrophilicity hydrophobicity immobilized cell membrane formation membrane permeability nonhuman polymerization porosity priority journal scanning electron microscopy sulfate reducing bacterium surface property Acidithiobacillus Bacteria Bacterial Adhesion Cells, Immobilized Dimethylpolysiloxanes Hydrophobic and Hydrophilic Interactions Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence Particle Size Polysaccharides, Bacterial Porosity Silanes Sulfates Surface Properties Bacteria (microorganisms)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09277765_v81_n1_p289_Fernandez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_09277765_v81_n1_p289_Fernandez2023-10-03T15:47:15Z PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition Fernández, M.R. Casabona, M.G. Anupama, V.N. Krishnakumar, B. Curutchet, G.A. Bernik, D.L. Bed material Biofilm CSLM PDMS SEM Acidithiobacillus ferrooxidans Bacterial biofilm Bacterial viability Bed materials Bed particles Biofilm formation Confocal laser scanning CSLM Hydrophilic groups Hydrophilic polymers Interconnected porous structure Particle surface PDMS Polydimethylsiloxane PDMS Polymer composition Porous particle SEM Sulphate-reducing bacteria Surface adsorption Tannery effluent Tetraethoxysilanes Xanthan Gum Adhesion Adsorption Bacteriology Biofilms Biofilters Cell adhesion Cell immobilization Fluorescence microscopy Hydrophilicity Microchannels Polymers Pore size Porous materials Scanning electron microscopy Silicones Surfaces dimeticone tetraethoxysilane xanthan Acidithiobacillus ferrooxidans article bacterial membrane bacterial strain bacterial viability bacterium adherence bacterium culture biofilm controlled study hydrophilicity hydrophobicity immobilized cell membrane formation membrane permeability nonhuman polymerization porosity priority journal scanning electron microscopy sulfate reducing bacterium surface property Acidithiobacillus Bacteria Bacterial Adhesion Biofilms Cells, Immobilized Dimethylpolysiloxanes Hydrophobic and Hydrophilic Interactions Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence Particle Size Polysaccharides, Bacterial Porosity Silanes Sulfates Surface Properties Acidithiobacillus ferrooxidans Bacteria (microorganisms) The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addition, PDMS materials can be cross-linked by copolymerization with other polymers. In the present work we have chosen two hydrophilic polymers: xanthan gum polysaccharide and tetraethoxysilane (TEOS). This versatile composition helps to modulate the interfacial hydrophobic/hydrophilic balance at the particle surface level and the roughness topology and pore size distribution, as revealed by scanning electron microscopy. Biofilm formation of a consortium isolated from a tannery effluent enriched in Sulphate Reducing Bacteria (SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles synthesized with pure PDMS, PDMS-xanthan gum and PDMS-TEOS hybrids. Bacterial viability assays using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on particle's surface significantly improves both cell adhesion and viability. © 2010 Elsevier B.V. Fil:Bernik, D.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09277765_v81_n1_p289_Fernandez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bed material Biofilm CSLM PDMS SEM Acidithiobacillus ferrooxidans Bacterial biofilm Bacterial viability Bed materials Bed particles Biofilm formation Confocal laser scanning CSLM Hydrophilic groups Hydrophilic polymers Interconnected porous structure Particle surface PDMS Polydimethylsiloxane PDMS Polymer composition Porous particle SEM Sulphate-reducing bacteria Surface adsorption Tannery effluent Tetraethoxysilanes Xanthan Gum Adhesion Adsorption Bacteriology Biofilms Biofilters Cell adhesion Cell immobilization Fluorescence microscopy Hydrophilicity Microchannels Polymers Pore size Porous materials Scanning electron microscopy Silicones Surfaces dimeticone tetraethoxysilane xanthan Acidithiobacillus ferrooxidans article bacterial membrane bacterial strain bacterial viability bacterium adherence bacterium culture biofilm controlled study hydrophilicity hydrophobicity immobilized cell membrane formation membrane permeability nonhuman polymerization porosity priority journal scanning electron microscopy sulfate reducing bacterium surface property Acidithiobacillus Bacteria Bacterial Adhesion Biofilms Cells, Immobilized Dimethylpolysiloxanes Hydrophobic and Hydrophilic Interactions Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence Particle Size Polysaccharides, Bacterial Porosity Silanes Sulfates Surface Properties Acidithiobacillus ferrooxidans Bacteria (microorganisms)
spellingShingle	Bed material Biofilm CSLM PDMS SEM Acidithiobacillus ferrooxidans Bacterial biofilm Bacterial viability Bed materials Bed particles Biofilm formation Confocal laser scanning CSLM Hydrophilic groups Hydrophilic polymers Interconnected porous structure Particle surface PDMS Polydimethylsiloxane PDMS Polymer composition Porous particle SEM Sulphate-reducing bacteria Surface adsorption Tannery effluent Tetraethoxysilanes Xanthan Gum Adhesion Adsorption Bacteriology Biofilms Biofilters Cell adhesion Cell immobilization Fluorescence microscopy Hydrophilicity Microchannels Polymers Pore size Porous materials Scanning electron microscopy Silicones Surfaces dimeticone tetraethoxysilane xanthan Acidithiobacillus ferrooxidans article bacterial membrane bacterial strain bacterial viability bacterium adherence bacterium culture biofilm controlled study hydrophilicity hydrophobicity immobilized cell membrane formation membrane permeability nonhuman polymerization porosity priority journal scanning electron microscopy sulfate reducing bacterium surface property Acidithiobacillus Bacteria Bacterial Adhesion Biofilms Cells, Immobilized Dimethylpolysiloxanes Hydrophobic and Hydrophilic Interactions Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence Particle Size Polysaccharides, Bacterial Porosity Silanes Sulfates Surface Properties Acidithiobacillus ferrooxidans Bacteria (microorganisms) Fernández, M.R. Casabona, M.G. Anupama, V.N. Krishnakumar, B. Curutchet, G.A. Bernik, D.L. PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
topic_facet	Bed material Biofilm CSLM PDMS SEM Acidithiobacillus ferrooxidans Bacterial biofilm Bacterial viability Bed materials Bed particles Biofilm formation Confocal laser scanning CSLM Hydrophilic groups Hydrophilic polymers Interconnected porous structure Particle surface PDMS Polydimethylsiloxane PDMS Polymer composition Porous particle SEM Sulphate-reducing bacteria Surface adsorption Tannery effluent Tetraethoxysilanes Xanthan Gum Adhesion Adsorption Bacteriology Biofilms Biofilters Cell adhesion Cell immobilization Fluorescence microscopy Hydrophilicity Microchannels Polymers Pore size Porous materials Scanning electron microscopy Silicones Surfaces dimeticone tetraethoxysilane xanthan Acidithiobacillus ferrooxidans article bacterial membrane bacterial strain bacterial viability bacterium adherence bacterium culture biofilm controlled study hydrophilicity hydrophobicity immobilized cell membrane formation membrane permeability nonhuman polymerization porosity priority journal scanning electron microscopy sulfate reducing bacterium surface property Acidithiobacillus Bacteria Bacterial Adhesion Biofilms Cells, Immobilized Dimethylpolysiloxanes Hydrophobic and Hydrophilic Interactions Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence Particle Size Polysaccharides, Bacterial Porosity Silanes Sulfates Surface Properties Acidithiobacillus ferrooxidans Bacteria (microorganisms)
description	The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addition, PDMS materials can be cross-linked by copolymerization with other polymers. In the present work we have chosen two hydrophilic polymers: xanthan gum polysaccharide and tetraethoxysilane (TEOS). This versatile composition helps to modulate the interfacial hydrophobic/hydrophilic balance at the particle surface level and the roughness topology and pore size distribution, as revealed by scanning electron microscopy. Biofilm formation of a consortium isolated from a tannery effluent enriched in Sulphate Reducing Bacteria (SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles synthesized with pure PDMS, PDMS-xanthan gum and PDMS-TEOS hybrids. Bacterial viability assays using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on particle's surface significantly improves both cell adhesion and viability. © 2010 Elsevier B.V.
format	JOUR
author	Fernández, M.R. Casabona, M.G. Anupama, V.N. Krishnakumar, B. Curutchet, G.A. Bernik, D.L.
author_facet	Fernández, M.R. Casabona, M.G. Anupama, V.N. Krishnakumar, B. Curutchet, G.A. Bernik, D.L.
author_sort	Fernández, M.R.
title	PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_short	PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_full	PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_fullStr	PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_full_unstemmed	PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_sort	pdms-based porous particles as support beds for cell immobilization: bacterial biofilm formation as a function of porosity and polymer composition
url	http://hdl.handle.net/20.500.12110/paper_09277765_v81_n1_p289_Fernandez
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_version_	1782030848325844992

PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition

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