Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro
The replication of herpes simplex virus (HSV) type 1 in Vero cells is inhibited in the presence of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one (6b), a synthetic brassinosteroid derivative. Attempts to disclose the mode of action of 6b indicate that a late step of virus multiplication is...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09248579_v23_n5_p524_Wachsman |
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todo:paper_09248579_v23_n5_p524_Wachsman2023-10-03T15:46:05Z Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro Wachsman, M.B. Castilla, V. Talarico, L.B. Ramirez, J.A. Galagovsky, L.R. Coto, C.E. (22S,23S)-3β- Bromo-5α,22,23-trihydroxystigmastan-6-one Antiviral Brassinosteroid derivatives Herpes simplex 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antivirus agent brassinosteroid unclassified drug animal cell antigen expression article controlled study drug mechanism Herpes simplex virus 1 immunofluorescence test in vitro study nonhuman priority journal protein synthesis Vero cell virus replication Animals Antigens, Viral Antiviral Agents Cercopithecus aethiops Cholestanones Fluorescent Antibody Technique, Indirect Herpesvirus 1, Human Transcription, Genetic Vero Cells Viral Proteins Virus Replication The replication of herpes simplex virus (HSV) type 1 in Vero cells is inhibited in the presence of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one (6b), a synthetic brassinosteroid derivative. Attempts to disclose the mode of action of 6b indicate that a late step of virus multiplication is affected. In the presence of 6b, HSV late protein synthesis was severely diminished and this inhibitory effect of 6b on HSV antigen expression was confirmed by immunofluorescence assays. © 2004 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramirez, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09248579_v23_n5_p524_Wachsman |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(22S,23S)-3β- Bromo-5α,22,23-trihydroxystigmastan-6-one Antiviral Brassinosteroid derivatives Herpes simplex 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antivirus agent brassinosteroid unclassified drug animal cell antigen expression article controlled study drug mechanism Herpes simplex virus 1 immunofluorescence test in vitro study nonhuman priority journal protein synthesis Vero cell virus replication Animals Antigens, Viral Antiviral Agents Cercopithecus aethiops Cholestanones Fluorescent Antibody Technique, Indirect Herpesvirus 1, Human Transcription, Genetic Vero Cells Viral Proteins Virus Replication |
| spellingShingle |
(22S,23S)-3β- Bromo-5α,22,23-trihydroxystigmastan-6-one Antiviral Brassinosteroid derivatives Herpes simplex 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antivirus agent brassinosteroid unclassified drug animal cell antigen expression article controlled study drug mechanism Herpes simplex virus 1 immunofluorescence test in vitro study nonhuman priority journal protein synthesis Vero cell virus replication Animals Antigens, Viral Antiviral Agents Cercopithecus aethiops Cholestanones Fluorescent Antibody Technique, Indirect Herpesvirus 1, Human Transcription, Genetic Vero Cells Viral Proteins Virus Replication Wachsman, M.B. Castilla, V. Talarico, L.B. Ramirez, J.A. Galagovsky, L.R. Coto, C.E. Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro |
| topic_facet |
(22S,23S)-3β- Bromo-5α,22,23-trihydroxystigmastan-6-one Antiviral Brassinosteroid derivatives Herpes simplex 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antivirus agent brassinosteroid unclassified drug animal cell antigen expression article controlled study drug mechanism Herpes simplex virus 1 immunofluorescence test in vitro study nonhuman priority journal protein synthesis Vero cell virus replication Animals Antigens, Viral Antiviral Agents Cercopithecus aethiops Cholestanones Fluorescent Antibody Technique, Indirect Herpesvirus 1, Human Transcription, Genetic Vero Cells Viral Proteins Virus Replication |
| description |
The replication of herpes simplex virus (HSV) type 1 in Vero cells is inhibited in the presence of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one (6b), a synthetic brassinosteroid derivative. Attempts to disclose the mode of action of 6b indicate that a late step of virus multiplication is affected. In the presence of 6b, HSV late protein synthesis was severely diminished and this inhibitory effect of 6b on HSV antigen expression was confirmed by immunofluorescence assays. © 2004 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved. |
| format |
JOUR |
| author |
Wachsman, M.B. Castilla, V. Talarico, L.B. Ramirez, J.A. Galagovsky, L.R. Coto, C.E. |
| author_facet |
Wachsman, M.B. Castilla, V. Talarico, L.B. Ramirez, J.A. Galagovsky, L.R. Coto, C.E. |
| author_sort |
Wachsman, M.B. |
| title |
Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro |
| title_short |
Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro |
| title_full |
Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro |
| title_fullStr |
Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro |
| title_full_unstemmed |
Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro |
| title_sort |
antiherpetic mode of action of (22s,23s)-3β-bromo-5α,22,23- trihydroxystigmastan-6-one in vitro |
| url |
http://hdl.handle.net/20.500.12110/paper_09248579_v23_n5_p524_Wachsman |
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