Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)

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In this work, Surface Enhanced Raman Spectroscopy (SERS) was employed in the taxonomic identification of fungi found in biofilms formed on earthen architecture walls (adobe, wattle and daub, and rammed earth) of historical buildings in the region known as Paraíba Valley (or São Paulo Historical Vall...

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Autores principales:	Fazio, A.T., López, M.M., Temperini, M.L.A., de Faria, D.L.A.
Formato:	JOUR
Materias:	Biodeterioration Earthen architecture Fungi Paraíba Valley Raman SERS Architecture Biodegradation Biofilms Deterioration Diagnosis Gold nanoparticles Landforms Light transmission Molecular biology Raman spectroscopy Substrates Walls (structural partitions) Earthen architectures Potato dextrose agars SERS-active substrates Surface enhanced Raman spectroscopy Taxonomic identifications Architectural acoustics
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09242031_v97_n_p129_Fazio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09242031_v97_n_p129_Fazio
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spelling	todo:paper_09242031_v97_n_p129_Fazio2023-10-03T15:45:57Z Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil) Fazio, A.T. López, M.M. Temperini, M.L.A. de Faria, D.L.A. Biodeterioration Earthen architecture Fungi Paraíba Valley Raman SERS Architecture Biodegradation Biofilms Deterioration Diagnosis Fungi Gold nanoparticles Landforms Light transmission Molecular biology Raman spectroscopy Substrates Walls (structural partitions) Biodeterioration Earthen architectures Potato dextrose agars Raman SERS SERS-active substrates Surface enhanced Raman spectroscopy Taxonomic identifications Architectural acoustics In this work, Surface Enhanced Raman Spectroscopy (SERS) was employed in the taxonomic identification of fungi found in biofilms formed on earthen architecture walls (adobe, wattle and daub, and rammed earth) of historical buildings in the region known as Paraíba Valley (or São Paulo Historical Valley), which are representative of the first phase of the Brazilian coffee cycle (1820–1880). Very few studies are reported in the literature where SERS-based techniques are used in fungi identification, most of them focused on clinical diagnosis. In the present investigation, pure colonies isolated from biofilms on earthen walls previously identified by classic taxonomy and molecular biology were selected. The genera were Trichoderma, Cladosporium, Aspergillus, Neurospora, Fusarium and Penicillium. The fungi were cultured on solid potato dextrose agar, extracted with ethyl acetate and the extracts were applied on dried Au nanoparticles. The SERS spectra exhibited bands in the 600–1800 cm−1 region which are characteristic of each genus, except Penicillium, as revealed by PCA statistical analysis. This work reports the use of a facile to prepare SERS-active substrate in the identification of microbial communities on earthen architecture walls and is the first step of an investigation aiming at the fast identification of fungi species from biofilms formed on earthen architecture buildings without the need of isolating the pure cultures. © 2018 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09242031_v97_n_p129_Fazio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biodeterioration Earthen architecture Fungi Paraíba Valley Raman SERS Architecture Biodegradation Biofilms Deterioration Diagnosis Fungi Gold nanoparticles Landforms Light transmission Molecular biology Raman spectroscopy Substrates Walls (structural partitions) Biodeterioration Earthen architectures Potato dextrose agars Raman SERS SERS-active substrates Surface enhanced Raman spectroscopy Taxonomic identifications Architectural acoustics
spellingShingle	Biodeterioration Earthen architecture Fungi Paraíba Valley Raman SERS Architecture Biodegradation Biofilms Deterioration Diagnosis Fungi Gold nanoparticles Landforms Light transmission Molecular biology Raman spectroscopy Substrates Walls (structural partitions) Biodeterioration Earthen architectures Potato dextrose agars Raman SERS SERS-active substrates Surface enhanced Raman spectroscopy Taxonomic identifications Architectural acoustics Fazio, A.T. López, M.M. Temperini, M.L.A. de Faria, D.L.A. Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
topic_facet	Biodeterioration Earthen architecture Fungi Paraíba Valley Raman SERS Architecture Biodegradation Biofilms Deterioration Diagnosis Fungi Gold nanoparticles Landforms Light transmission Molecular biology Raman spectroscopy Substrates Walls (structural partitions) Biodeterioration Earthen architectures Potato dextrose agars Raman SERS SERS-active substrates Surface enhanced Raman spectroscopy Taxonomic identifications Architectural acoustics
description	In this work, Surface Enhanced Raman Spectroscopy (SERS) was employed in the taxonomic identification of fungi found in biofilms formed on earthen architecture walls (adobe, wattle and daub, and rammed earth) of historical buildings in the region known as Paraíba Valley (or São Paulo Historical Valley), which are representative of the first phase of the Brazilian coffee cycle (1820–1880). Very few studies are reported in the literature where SERS-based techniques are used in fungi identification, most of them focused on clinical diagnosis. In the present investigation, pure colonies isolated from biofilms on earthen walls previously identified by classic taxonomy and molecular biology were selected. The genera were Trichoderma, Cladosporium, Aspergillus, Neurospora, Fusarium and Penicillium. The fungi were cultured on solid potato dextrose agar, extracted with ethyl acetate and the extracts were applied on dried Au nanoparticles. The SERS spectra exhibited bands in the 600–1800 cm−1 region which are characteristic of each genus, except Penicillium, as revealed by PCA statistical analysis. This work reports the use of a facile to prepare SERS-active substrate in the identification of microbial communities on earthen architecture walls and is the first step of an investigation aiming at the fast identification of fungi species from biofilms formed on earthen architecture buildings without the need of isolating the pure cultures. © 2018 Elsevier B.V.
format	JOUR
author	Fazio, A.T. López, M.M. Temperini, M.L.A. de Faria, D.L.A.
author_facet	Fazio, A.T. López, M.M. Temperini, M.L.A. de Faria, D.L.A.
author_sort	Fazio, A.T.
title	Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_short	Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_full	Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_fullStr	Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_full_unstemmed	Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_sort	surface enhanced raman spectroscopy and cultural heritage biodeterioration: fungi identification in earthen architecture from paraíba valley (são paulo, brazil)
url	http://hdl.handle.net/20.500.12110/paper_09242031_v97_n_p129_Fazio
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Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)

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