Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis
Diesel is a widely distributed pollutant. Bioremediation of this kind of compounds requires the use of microorganisms able to survive and adapt to contaminated environments. Pseudomonas extremaustralis is an Antarctic bacterium with a remarkable survival capability associated to polyhydroxyalkanoate...
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todo:paper_09239820_v23_n5_p645_Tribelli2023-10-03T15:45:56Z Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis Tribelli, P.M. Di Martino, C. López, N.I. Raiger Iustman, L.J. Biofilm Biosurfactant production Diesel bioremediation Polyhydroxyalkanoates Pseudomonas Antarctic bacteria Biofilm cells Biosurfactant production Branched alkanes Contaminated environment Extreme environment Flask culture Hydrocarbon bioremediation Inocula Poly-hydroxyalkanoate Polyhydroxyalkanoates Pseudomonas Bacteria Biomolecules Bioremediation Biotechnology Bottles Cell culture Cell growth Degradation Growth kinetics Paraffins Pollution Surface active agents Biofilms alkane carbon gasoline polyhydroxyalkanoic acid surfactant alkane bacterium biofilm biogenic material bioremediation biotechnology cytology diesel ester industrial production microbial activity pollution tolerance polymer surfactant survivorship Antarctica article bacterial gene biofilm bioremediation biosynthesis drug effect genetics growth, development and aging metabolism microbiology phylogeny physiology Pseudomonas surface tension Alkanes Antarctic Regions Biodegradation, Environmental Biofilms Carbon Gasoline Genes, Bacterial Phylogeny Polyhydroxyalkanoates Pseudomonas Surface Tension Surface-Active Agents Bacteria (microorganisms) Pseudomonas Diesel is a widely distributed pollutant. Bioremediation of this kind of compounds requires the use of microorganisms able to survive and adapt to contaminated environments. Pseudomonas extremaustralis is an Antarctic bacterium with a remarkable survival capability associated to polyhydroxyalkanoates (PHAs) production. This strain was used to investigate the effect of cell growth conditions-in biofilm versus shaken flask cultures-as well as the inocula characteristics associated with PHAs accumulation, on diesel degradation. Biofilms showed increased cell growth, biosurfactant production and diesel degradation compared with that obtained in shaken flask cultures. PHA accumulation decreased biofilm cell attachment and enhanced biosurfactant production. Degradation of long-chain and branched alkanes was observed in biofilms, while in shaken flasks only medium-chain length alkanes were degraded. This work shows that the PHA accumulating bacterium P. extremaustralis can be a good candidate to be used as hydrocarbon bioremediation agent, especially in extreme environments. © 2012 Springer Science+Business Media B.V. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Raiger Iustman, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09239820_v23_n5_p645_Tribelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biofilm Biosurfactant production Diesel bioremediation Polyhydroxyalkanoates Pseudomonas Antarctic bacteria Biofilm cells Biosurfactant production Branched alkanes Contaminated environment Extreme environment Flask culture Hydrocarbon bioremediation Inocula Poly-hydroxyalkanoate Polyhydroxyalkanoates Pseudomonas Bacteria Biomolecules Bioremediation Biotechnology Bottles Cell culture Cell growth Degradation Growth kinetics Paraffins Pollution Surface active agents Biofilms alkane carbon gasoline polyhydroxyalkanoic acid surfactant alkane bacterium biofilm biogenic material bioremediation biotechnology cytology diesel ester industrial production microbial activity pollution tolerance polymer surfactant survivorship Antarctica article bacterial gene biofilm bioremediation biosynthesis drug effect genetics growth, development and aging metabolism microbiology phylogeny physiology Pseudomonas surface tension Alkanes Antarctic Regions Biodegradation, Environmental Biofilms Carbon Gasoline Genes, Bacterial Phylogeny Polyhydroxyalkanoates Pseudomonas Surface Tension Surface-Active Agents Bacteria (microorganisms) Pseudomonas |
| spellingShingle |
Biofilm Biosurfactant production Diesel bioremediation Polyhydroxyalkanoates Pseudomonas Antarctic bacteria Biofilm cells Biosurfactant production Branched alkanes Contaminated environment Extreme environment Flask culture Hydrocarbon bioremediation Inocula Poly-hydroxyalkanoate Polyhydroxyalkanoates Pseudomonas Bacteria Biomolecules Bioremediation Biotechnology Bottles Cell culture Cell growth Degradation Growth kinetics Paraffins Pollution Surface active agents Biofilms alkane carbon gasoline polyhydroxyalkanoic acid surfactant alkane bacterium biofilm biogenic material bioremediation biotechnology cytology diesel ester industrial production microbial activity pollution tolerance polymer surfactant survivorship Antarctica article bacterial gene biofilm bioremediation biosynthesis drug effect genetics growth, development and aging metabolism microbiology phylogeny physiology Pseudomonas surface tension Alkanes Antarctic Regions Biodegradation, Environmental Biofilms Carbon Gasoline Genes, Bacterial Phylogeny Polyhydroxyalkanoates Pseudomonas Surface Tension Surface-Active Agents Bacteria (microorganisms) Pseudomonas Tribelli, P.M. Di Martino, C. López, N.I. Raiger Iustman, L.J. Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis |
| topic_facet |
Biofilm Biosurfactant production Diesel bioremediation Polyhydroxyalkanoates Pseudomonas Antarctic bacteria Biofilm cells Biosurfactant production Branched alkanes Contaminated environment Extreme environment Flask culture Hydrocarbon bioremediation Inocula Poly-hydroxyalkanoate Polyhydroxyalkanoates Pseudomonas Bacteria Biomolecules Bioremediation Biotechnology Bottles Cell culture Cell growth Degradation Growth kinetics Paraffins Pollution Surface active agents Biofilms alkane carbon gasoline polyhydroxyalkanoic acid surfactant alkane bacterium biofilm biogenic material bioremediation biotechnology cytology diesel ester industrial production microbial activity pollution tolerance polymer surfactant survivorship Antarctica article bacterial gene biofilm bioremediation biosynthesis drug effect genetics growth, development and aging metabolism microbiology phylogeny physiology Pseudomonas surface tension Alkanes Antarctic Regions Biodegradation, Environmental Biofilms Carbon Gasoline Genes, Bacterial Phylogeny Polyhydroxyalkanoates Pseudomonas Surface Tension Surface-Active Agents Bacteria (microorganisms) Pseudomonas |
| description |
Diesel is a widely distributed pollutant. Bioremediation of this kind of compounds requires the use of microorganisms able to survive and adapt to contaminated environments. Pseudomonas extremaustralis is an Antarctic bacterium with a remarkable survival capability associated to polyhydroxyalkanoates (PHAs) production. This strain was used to investigate the effect of cell growth conditions-in biofilm versus shaken flask cultures-as well as the inocula characteristics associated with PHAs accumulation, on diesel degradation. Biofilms showed increased cell growth, biosurfactant production and diesel degradation compared with that obtained in shaken flask cultures. PHA accumulation decreased biofilm cell attachment and enhanced biosurfactant production. Degradation of long-chain and branched alkanes was observed in biofilms, while in shaken flasks only medium-chain length alkanes were degraded. This work shows that the PHA accumulating bacterium P. extremaustralis can be a good candidate to be used as hydrocarbon bioremediation agent, especially in extreme environments. © 2012 Springer Science+Business Media B.V. |
| format |
JOUR |
| author |
Tribelli, P.M. Di Martino, C. López, N.I. Raiger Iustman, L.J. |
| author_facet |
Tribelli, P.M. Di Martino, C. López, N.I. Raiger Iustman, L.J. |
| author_sort |
Tribelli, P.M. |
| title |
Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis |
| title_short |
Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis |
| title_full |
Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis |
| title_fullStr |
Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis |
| title_full_unstemmed |
Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis |
| title_sort |
biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the antarctic polyhydroxyalkanoate producer pseudomonas extremaustralis |
| url |
http://hdl.handle.net/20.500.12110/paper_09239820_v23_n5_p645_Tribelli |
| work_keys_str_mv |
AT tribellipm biofilmlifestyleenhancesdieselbioremediationandbiosurfactantproductionintheantarcticpolyhydroxyalkanoateproducerpseudomonasextremaustralis AT dimartinoc biofilmlifestyleenhancesdieselbioremediationandbiosurfactantproductionintheantarcticpolyhydroxyalkanoateproducerpseudomonasextremaustralis AT lopezni biofilmlifestyleenhancesdieselbioremediationandbiosurfactantproductionintheantarcticpolyhydroxyalkanoateproducerpseudomonasextremaustralis AT raigeriustmanlj biofilmlifestyleenhancesdieselbioremediationandbiosurfactantproductionintheantarcticpolyhydroxyalkanoateproducerpseudomonasextremaustralis |
| _version_ |
1782025787893874688 |