Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites

To study the importance of the bone marrow in the long-term antibody response, IgG and IgA antitoxin antibody-forming cells were evaluated by ELISPOT in Peyer's patches, mesenteric lymph nodes, spleen, lamina propria of the small intestine and bone marrow at several times after oral immunizatio...

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Autores principales:	Benedetti, R., Lev, P., Massouh, E., Fló, J.
Formato:	JOUR
Materias:	Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09232494_v149_n2_p107_Benedetti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09232494_v149_n2_p107_Benedetti
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spelling	todo:paper_09232494_v149_n2_p107_Benedetti2023-10-03T15:45:51Z Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites Benedetti, R. Lev, P. Massouh, E. Fló, J. Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte To study the importance of the bone marrow in the long-term antibody response, IgG and IgA antitoxin antibody-forming cells were evaluated by ELISPOT in Peyer's patches, mesenteric lymph nodes, spleen, lamina propria of the small intestine and bone marrow at several times after oral immunization with cholera toxin. The mesenteric lymph node was the site having the major frequency of IgG antitoxin during the first two weeks after priming, whereas lamina propria was the site with e major number of IgA antitoxin antibody- forming cells. However, from 3 weeks until 10 months after priming, bone marrow became the site with the major frequency of IgG, and especially IgA antitoxin antibody-forming cells (without taking into account the lamina propria). This result indicates that bone marrow was responsible for the long-term antibody response and raises questions concerning the mechanisms involved in the maintenance of antibody production. The importance of bone marrow as a site of antibody production was great when we analysed results as the true contribution of the total number of antitoxin antibody-forming cells, taking into account the number of cells recovered from each organ. When we analysed the anatomical location of memory B and T cells by adoptive transference, we found that cells from mesenteric lymph nodes and spleen were able to transfer a strong antibody response to naive syngeneic recipients, whereas bone marrow cells transferred a weak antibody response. Fil:Benedetti, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lev, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Massouh, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09232494_v149_n2_p107_Benedetti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte
spellingShingle	Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte Benedetti, R. Lev, P. Massouh, E. Fló, J. Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites
topic_facet	Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte
description	To study the importance of the bone marrow in the long-term antibody response, IgG and IgA antitoxin antibody-forming cells were evaluated by ELISPOT in Peyer's patches, mesenteric lymph nodes, spleen, lamina propria of the small intestine and bone marrow at several times after oral immunization with cholera toxin. The mesenteric lymph node was the site having the major frequency of IgG antitoxin during the first two weeks after priming, whereas lamina propria was the site with e major number of IgA antitoxin antibody- forming cells. However, from 3 weeks until 10 months after priming, bone marrow became the site with the major frequency of IgG, and especially IgA antitoxin antibody-forming cells (without taking into account the lamina propria). This result indicates that bone marrow was responsible for the long-term antibody response and raises questions concerning the mechanisms involved in the maintenance of antibody production. The importance of bone marrow as a site of antibody production was great when we analysed results as the true contribution of the total number of antitoxin antibody-forming cells, taking into account the number of cells recovered from each organ. When we analysed the anatomical location of memory B and T cells by adoptive transference, we found that cells from mesenteric lymph nodes and spleen were able to transfer a strong antibody response to naive syngeneic recipients, whereas bone marrow cells transferred a weak antibody response.
format	JOUR
author	Benedetti, R. Lev, P. Massouh, E. Fló, J.
author_facet	Benedetti, R. Lev, P. Massouh, E. Fló, J.
author_sort	Benedetti, R.
title	Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites
title_short	Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites
title_full	Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites
title_fullStr	Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites
title_full_unstemmed	Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites
title_sort	long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory b-cell maintenance at systemic and mucosal sites
url	http://hdl.handle.net/20.500.12110/paper_09232494_v149_n2_p107_Benedetti
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Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites

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