A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)

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The pseudofilamentous red alga Chroodactylon ornatum was grown in f/2 culture medium with the addition of 10, 34, and 100 μM (nominal concentration) cupric sulfate. The bioassays were terminated at two selected end points (days 4 and 12). Growth inhibition, changes in pigment composition, and oxidat...

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Autores principales: Cabrera, J.M., Stortz, C.A., Rodríguez, M.C.
Formato: INPR
Lenguaje:English
Materias:
Biosorption
Chroodactylon ornatum
Copper bioassays
Mucilage
Sulfated galactan
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09218971_v_n_p1_Cabrera
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_09218971_v_n_p1_Cabrera2023-10-03T15:45:45Z A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta) Cabrera, J.M. Stortz, C.A. Rodríguez, M.C. Biosorption Chroodactylon ornatum Copper bioassays Mucilage Sulfated galactan The pseudofilamentous red alga Chroodactylon ornatum was grown in f/2 culture medium with the addition of 10, 34, and 100 μM (nominal concentration) cupric sulfate. The bioassays were terminated at two selected end points (days 4 and 12). Growth inhibition, changes in pigment composition, and oxidative stress indicators such as phenolic compounds and lipid peroxidation (dosed as thiobarbituric reactive substances) were observed in cultures with 34 and 100-μM cupric sulfate. Quinacrine (Atebrin) and chlorotetracycline fluorochromes showed abundant vacuoles of acidic content, related with mucilage secretion. Structural analyses by methylation, desulfation-methylation, alkaline treatment, and NMR spectroscopy revealed that the mucilaginous sheath of C. ornatum contains a sulfated galactan with a backbone of alternating 3-linked β-d-galactopyranose and 4-linked α-l-galactopyranose moieties, i.e., an agaran. The absence of 3,6-anhydrogalactose and of its precursor unit (α-galactose 6-sulfate) were confirmed. The highly sulfated polysaccharide contained these ester groups on the C-2 and C-4 hydroxyl groups of the 3-linked unit and on C-3 of the 4-linked units. The large proportion of sulfate esterification in this polysaccharide can be related to the extracellular biosorption of copper divalent cation (2.5 ± 0.4 mg per gram of dry weight) and to copper tolerance in bioassays. © 2014 Springer Science+Business Media Dordrecht. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. INPR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09218971_v_n_p1_Cabrera
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language English
orig_language_str_mv English
topic Biosorption
Chroodactylon ornatum
Copper bioassays
Mucilage
Sulfated galactan
spellingShingle Biosorption
Chroodactylon ornatum
Copper bioassays
Mucilage
Sulfated galactan
Cabrera, J.M.
Stortz, C.A.
Rodríguez, M.C.
A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)
topic_facet Biosorption
Chroodactylon ornatum
Copper bioassays
Mucilage
Sulfated galactan
description The pseudofilamentous red alga Chroodactylon ornatum was grown in f/2 culture medium with the addition of 10, 34, and 100 μM (nominal concentration) cupric sulfate. The bioassays were terminated at two selected end points (days 4 and 12). Growth inhibition, changes in pigment composition, and oxidative stress indicators such as phenolic compounds and lipid peroxidation (dosed as thiobarbituric reactive substances) were observed in cultures with 34 and 100-μM cupric sulfate. Quinacrine (Atebrin) and chlorotetracycline fluorochromes showed abundant vacuoles of acidic content, related with mucilage secretion. Structural analyses by methylation, desulfation-methylation, alkaline treatment, and NMR spectroscopy revealed that the mucilaginous sheath of C. ornatum contains a sulfated galactan with a backbone of alternating 3-linked β-d-galactopyranose and 4-linked α-l-galactopyranose moieties, i.e., an agaran. The absence of 3,6-anhydrogalactose and of its precursor unit (α-galactose 6-sulfate) were confirmed. The highly sulfated polysaccharide contained these ester groups on the C-2 and C-4 hydroxyl groups of the 3-linked unit and on C-3 of the 4-linked units. The large proportion of sulfate esterification in this polysaccharide can be related to the extracellular biosorption of copper divalent cation (2.5 ± 0.4 mg per gram of dry weight) and to copper tolerance in bioassays. © 2014 Springer Science+Business Media Dordrecht.
format INPR
author Cabrera, J.M.
Stortz, C.A.
Rodríguez, M.C.
author_facet Cabrera, J.M.
Stortz, C.A.
Rodríguez, M.C.
author_sort Cabrera, J.M.
title A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)
title_short A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)
title_full A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)
title_fullStr A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)
title_full_unstemmed A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)
title_sort sulfated galactan from the mucilaginous sheath of the red filamentous alga chroodactylon ornatum (stylonematophyceae, rhodophyta)
url http://hdl.handle.net/20.500.12110/paper_09218971_v_n_p1_Cabrera
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AT rodriguezmc sulfatedgalactanfromthemucilaginoussheathoftheredfilamentousalgachroodactylonornatumstylonematophyceaerhodophyta
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