Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein

Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications,...

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Autores principales:	Distéfano, A.J., Maldonado, S., Hopp, H.E., Del Vas, M.
Formato:	JOUR
Materias:	Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Zea mays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09208569_v38_n3_p455_Distefano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_09208569_v38_n3_p455_Distefano2023-10-03T15:44:59Z Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein Distéfano, A.J. Maldonado, S. Hopp, H.E. Del Vas, M. Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein. © 2009 Springer Science+Business Media, LLC. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09208569_v38_n3_p455_Distefano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays
spellingShingle	Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays Distéfano, A.J. Maldonado, S. Hopp, H.E. Del Vas, M. Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
topic_facet	Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays
description	Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein. © 2009 Springer Science+Business Media, LLC.
format	JOUR
author	Distéfano, A.J. Maldonado, S. Hopp, H.E. Del Vas, M.
author_facet	Distéfano, A.J. Maldonado, S. Hopp, H.E. Del Vas, M.
author_sort	Distéfano, A.J.
title	Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_short	Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_full	Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_fullStr	Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_full_unstemmed	Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_sort	mal de río cuarto virus (mrcv) genomic segment s3 codes for the major core capsid protein
url	http://hdl.handle.net/20.500.12110/paper_09208569_v38_n3_p455_Distefano
work_keys_str_mv	AT distefanoaj malderiocuartovirusmrcvgenomicsegments3codesforthemajorcorecapsidprotein AT maldonados malderiocuartovirusmrcvgenomicsegments3codesforthemajorcorecapsidprotein AT hopphe malderiocuartovirusmrcvgenomicsegments3codesforthemajorcorecapsidprotein AT delvasm malderiocuartovirusmrcvgenomicsegments3codesforthemajorcorecapsidprotein
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Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein

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