Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)

This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final matu...

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Detalles Bibliográficos
Autores principales:	Ravaglia, M.A., Lo Nostro, F.L., Maggese, M.C., Guerrero, G.A., Somoza, G.M.
Formato:	JOUR
Materias:	Diandric Domperidone Fish GnRH Hermaphroditism Protogynous Sex reversal Spermiation Synbranchidae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09201742_v16_n5_p425_Ravaglia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09201742_v16_n5_p425_Ravaglia
record_format	dspace
spelling	todo:paper_09201742_v16_n5_p425_Ravaglia2023-10-03T15:44:45Z Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae) Ravaglia, M.A. Lo Nostro, F.L. Maggese, M.C. Guerrero, G.A. Somoza, G.M. Diandric Domperidone Fish GnRH Hermaphroditism Protogynous Sex reversal Spermiation Synbranchidae This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final maturation and gamete release in this species. Evidence for the presence of two GnRH variants, sGnRH and cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay with different antisera. The effects of treatment with sGnRH-A+DOM were checked by three ways: oocyte and milt release by stripping, histological analysis of the gonadal tissue, and androgen serum levels at different times throughout the experiment. In males, spermiation was induced after three weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation at the end of the experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal. All the treated fish had elevated androgen serum levels from the third week, with respect to control fish. In all cases, serum estradiol levels were undetectable. These results suggest that treatment with sGnRH analog and DOM induce sex reversal in female and spermiation in males. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09201742_v16_n5_p425_Ravaglia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Diandric Domperidone Fish GnRH Hermaphroditism Protogynous Sex reversal Spermiation Synbranchidae
spellingShingle	Diandric Domperidone Fish GnRH Hermaphroditism Protogynous Sex reversal Spermiation Synbranchidae Ravaglia, M.A. Lo Nostro, F.L. Maggese, M.C. Guerrero, G.A. Somoza, G.M. Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
topic_facet	Diandric Domperidone Fish GnRH Hermaphroditism Protogynous Sex reversal Spermiation Synbranchidae
description	This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final maturation and gamete release in this species. Evidence for the presence of two GnRH variants, sGnRH and cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay with different antisera. The effects of treatment with sGnRH-A+DOM were checked by three ways: oocyte and milt release by stripping, histological analysis of the gonadal tissue, and androgen serum levels at different times throughout the experiment. In males, spermiation was induced after three weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation at the end of the experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal. All the treated fish had elevated androgen serum levels from the third week, with respect to control fish. In all cases, serum estradiol levels were undetectable. These results suggest that treatment with sGnRH analog and DOM induce sex reversal in female and spermiation in males.
format	JOUR
author	Ravaglia, M.A. Lo Nostro, F.L. Maggese, M.C. Guerrero, G.A. Somoza, G.M.
author_facet	Ravaglia, M.A. Lo Nostro, F.L. Maggese, M.C. Guerrero, G.A. Somoza, G.M.
author_sort	Ravaglia, M.A.
title	Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_short	Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_full	Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_fullStr	Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_full_unstemmed	Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_sort	characterization of molecular variants of gnrh, induction of spermiation and sex reversal using salmon gnrh-a and domperidone in the protogynous diandric fish, synbranchus marmoratus bloch, (teleostei, synbranchidae)
url	http://hdl.handle.net/20.500.12110/paper_09201742_v16_n5_p425_Ravaglia
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Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)

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