The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)

The mast cell product tryptase, via protease-activated receptor 2 (PAR2), induces cyclooxygenase-2 (COX2) and 15-deoxy-prostaglandin J2 (15d-PGJ2) synthesis. 15d-PGJ2, through the nuclear peroxisome proliferator activated receptor gamma (PPARγ), subsequently causes fibroblast proliferation. In this...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Frungieri, M.B., Albrecht, M., Raemsch, R., Mayerhofer, A.
Formato:	JOUR
Materias:	Ca 2+ cAMP Cyclooxygenase-2 MAP kinases Transcription factors Tryptase 2 (2 amino 3 methoxyphenyl)chromone cyclooxygenase 2 mitogen activated protein kinase 1 mitogen activated protein kinase inhibitor phosphotransferase prostaglandin derivative protein c fos stress activated protein kinase tryptase article calcium cell level cell proliferation controlled study DNA microarray enzyme activation enzyme activity enzyme induction enzyme mechanism fibroblast human human cell mast cell priority journal protein expression protein phosphorylation reverse transcription polymerase chain reaction signal transduction upregulation Western blotting Base Sequence Calcium Cell Proliferation Cyclic AMP Cyclooxygenase 2 Fibroblasts Humans Male Mast Cells Membrane Proteins Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Prostaglandin-Endoperoxide Synthases Protein Isoforms Serine Endopeptidases Signal Transduction Tryptases Up-Regulation
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_08986568_v17_n4_p525_Frungieri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_08986568_v17_n4_p525_Frungieri
record_format	dspace
spelling	todo:paper_08986568_v17_n4_p525_Frungieri2023-10-03T15:44:01Z The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2) Frungieri, M.B. Albrecht, M. Raemsch, R. Mayerhofer, A. Ca 2+ cAMP Cyclooxygenase-2 MAP kinases Transcription factors Tryptase 2 (2 amino 3 methoxyphenyl)chromone cyclooxygenase 2 mitogen activated protein kinase 1 mitogen activated protein kinase inhibitor phosphotransferase prostaglandin derivative protein c fos stress activated protein kinase tryptase article calcium cell level cell proliferation controlled study DNA microarray enzyme activation enzyme activity enzyme induction enzyme mechanism fibroblast human human cell mast cell priority journal protein expression protein phosphorylation reverse transcription polymerase chain reaction signal transduction upregulation Western blotting Base Sequence Calcium Cell Proliferation Cyclic AMP Cyclooxygenase 2 Fibroblasts Humans Male Mast Cells Membrane Proteins Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Prostaglandin-Endoperoxide Synthases Protein Isoforms Serine Endopeptidases Signal Transduction Tryptases Up-Regulation The mast cell product tryptase, via protease-activated receptor 2 (PAR2), induces cyclooxygenase-2 (COX2) and 15-deoxy-prostaglandin J2 (15d-PGJ2) synthesis. 15d-PGJ2, through the nuclear peroxisome proliferator activated receptor gamma (PPARγ), subsequently causes fibroblast proliferation. In this study we attempted to determine initial events of the tryptase/PAR2 signaling pathway leading to COX2 induction and fibroblast proliferation. In human fibroblasts (HFFF2), cDNA array, RT-PCR and Western blotting studies demonstrated that tryptase, but not 15d-PGJ2, up-regulates c-jun, c-fos and COX2 expression, and phosphorylates the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2). Furthermore, tryptase effects on erk1/2, c-jun, c-fos, COX2 and cell proliferation were prevented by PD98059, an inhibitor of the mitogen-activated protein kinase kinase (MEK). Other kinases [P38, stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JUNK), erk5], intracellular Ca 2+ or cAMP were not affected by tryptase/PAR2. Our study identifies crucial intracellular events leading to induction of COX2 and fibroblast proliferation, i.e. a cornerstone of fibrosis. © 2004 Elsevier Inc. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08986568_v17_n4_p525_Frungieri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Ca 2+ cAMP Cyclooxygenase-2 MAP kinases Transcription factors Tryptase 2 (2 amino 3 methoxyphenyl)chromone cyclooxygenase 2 mitogen activated protein kinase 1 mitogen activated protein kinase inhibitor phosphotransferase prostaglandin derivative protein c fos stress activated protein kinase tryptase article calcium cell level cell proliferation controlled study DNA microarray enzyme activation enzyme activity enzyme induction enzyme mechanism fibroblast human human cell mast cell priority journal protein expression protein phosphorylation reverse transcription polymerase chain reaction signal transduction upregulation Western blotting Base Sequence Calcium Cell Proliferation Cyclic AMP Cyclooxygenase 2 Fibroblasts Humans Male Mast Cells Membrane Proteins Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Prostaglandin-Endoperoxide Synthases Protein Isoforms Serine Endopeptidases Signal Transduction Tryptases Up-Regulation
spellingShingle	Ca 2+ cAMP Cyclooxygenase-2 MAP kinases Transcription factors Tryptase 2 (2 amino 3 methoxyphenyl)chromone cyclooxygenase 2 mitogen activated protein kinase 1 mitogen activated protein kinase inhibitor phosphotransferase prostaglandin derivative protein c fos stress activated protein kinase tryptase article calcium cell level cell proliferation controlled study DNA microarray enzyme activation enzyme activity enzyme induction enzyme mechanism fibroblast human human cell mast cell priority journal protein expression protein phosphorylation reverse transcription polymerase chain reaction signal transduction upregulation Western blotting Base Sequence Calcium Cell Proliferation Cyclic AMP Cyclooxygenase 2 Fibroblasts Humans Male Mast Cells Membrane Proteins Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Prostaglandin-Endoperoxide Synthases Protein Isoforms Serine Endopeptidases Signal Transduction Tryptases Up-Regulation Frungieri, M.B. Albrecht, M. Raemsch, R. Mayerhofer, A. The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
topic_facet	Ca 2+ cAMP Cyclooxygenase-2 MAP kinases Transcription factors Tryptase 2 (2 amino 3 methoxyphenyl)chromone cyclooxygenase 2 mitogen activated protein kinase 1 mitogen activated protein kinase inhibitor phosphotransferase prostaglandin derivative protein c fos stress activated protein kinase tryptase article calcium cell level cell proliferation controlled study DNA microarray enzyme activation enzyme activity enzyme induction enzyme mechanism fibroblast human human cell mast cell priority journal protein expression protein phosphorylation reverse transcription polymerase chain reaction signal transduction upregulation Western blotting Base Sequence Calcium Cell Proliferation Cyclic AMP Cyclooxygenase 2 Fibroblasts Humans Male Mast Cells Membrane Proteins Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Prostaglandin-Endoperoxide Synthases Protein Isoforms Serine Endopeptidases Signal Transduction Tryptases Up-Regulation
description	The mast cell product tryptase, via protease-activated receptor 2 (PAR2), induces cyclooxygenase-2 (COX2) and 15-deoxy-prostaglandin J2 (15d-PGJ2) synthesis. 15d-PGJ2, through the nuclear peroxisome proliferator activated receptor gamma (PPARγ), subsequently causes fibroblast proliferation. In this study we attempted to determine initial events of the tryptase/PAR2 signaling pathway leading to COX2 induction and fibroblast proliferation. In human fibroblasts (HFFF2), cDNA array, RT-PCR and Western blotting studies demonstrated that tryptase, but not 15d-PGJ2, up-regulates c-jun, c-fos and COX2 expression, and phosphorylates the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2). Furthermore, tryptase effects on erk1/2, c-jun, c-fos, COX2 and cell proliferation were prevented by PD98059, an inhibitor of the mitogen-activated protein kinase kinase (MEK). Other kinases [P38, stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JUNK), erk5], intracellular Ca 2+ or cAMP were not affected by tryptase/PAR2. Our study identifies crucial intracellular events leading to induction of COX2 and fibroblast proliferation, i.e. a cornerstone of fibrosis. © 2004 Elsevier Inc. All rights reserved.
format	JOUR
author	Frungieri, M.B. Albrecht, M. Raemsch, R. Mayerhofer, A.
author_facet	Frungieri, M.B. Albrecht, M. Raemsch, R. Mayerhofer, A.
author_sort	Frungieri, M.B.
title	The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
title_short	The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
title_full	The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
title_fullStr	The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
title_full_unstemmed	The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
title_sort	action of the mast cell product tryptase on cyclooxygenase-2 (cox2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
url	http://hdl.handle.net/20.500.12110/paper_08986568_v17_n4_p525_Frungieri
work_keys_str_mv	AT frungierimb theactionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12 AT albrechtm theactionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12 AT raemschr theactionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12 AT mayerhofera theactionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12 AT frungierimb actionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12 AT albrechtm actionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12 AT raemschr actionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12 AT mayerhofera actionofthemastcellproducttryptaseoncyclooxygenase2cox2andsubsequentfibroblastproliferationinvolvesactivationoftheextracellularsignalregulatedkinaseisoforms1and2erk12
_version_	1807318405592645632

The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)

Ejemplares similares