Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris
Xanthan, the main exopolysaccharide (EPS) synthesized by Xanthomonas spp., contributes to bacterial stress tolerance and enhances attachment to plant surfaces by helping in biofilm formation. Therefore, xanthan is essential for successful colonization and growth in planta and has also been proposed...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_08940282_v29_n9_p688_Bianco |
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todo:paper_08940282_v29_n9_p688_Bianco2023-10-03T15:41:55Z Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris Bianco, M.I. Toum, L. Yaryura, P.M. Mielnichuk, N. Gudesblat, G.E. Roeschlin, R. Marano, M.R. Ielpi, L. Vojnov, A.A. bacterial polysaccharide callose glucan pyruvic acid virulence factor xanthan Arabidopsis biofilm chemistry genetics growth, development and aging host pathogen interaction metabolism microbiology mutation pathogenicity physiology plant disease plant leaf plant stoma virulence Xanthomonas campestris Arabidopsis Biofilms Glucans Host-Pathogen Interactions Mutation Plant Diseases Plant Leaves Plant Stomata Polysaccharides, Bacterial Pyruvic Acid Virulence Virulence Factors Xanthomonas campestris Xanthan, the main exopolysaccharide (EPS) synthesized by Xanthomonas spp., contributes to bacterial stress tolerance and enhances attachment to plant surfaces by helping in biofilm formation. Therefore, xanthan is essential for successful colonization and growth in planta and has also been proposed to be involved in the promotion of pathogenesis by calcium ion chelation and, hence, in the suppression of the plant defense responses in which this cation acts as a signal. The aim of this work was to study the relationship between xanthan structure and its role as a virulence factor. We analyzed four Xanthomonas campestris pv. campestris mutants that synthesize structural variants of xanthan. We found that the lack of acetyl groups that decorate the internal mannose residues, ketal-pyruvate groups, and external mannose residues affects bacterial adhesion and biofilm architecture. In addition, the mutants that synthesized EPS without pyruvilation or without the external mannose residues did not develop disease symptoms in Arabidopsis thaliana. We also observed that the presence of the external mannose residues and, hence, pyruvilation is required for xanthan to suppress callose deposition as well as to interfere with stomatal defense. In conclusion, pyruvilation of xanthan seems to be essential for Xanthomonas campestris pv. campestris virulence. © 2016 The American Phytopathological Society. Fil:Mielnichuk, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vojnov, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08940282_v29_n9_p688_Bianco |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial polysaccharide callose glucan pyruvic acid virulence factor xanthan Arabidopsis biofilm chemistry genetics growth, development and aging host pathogen interaction metabolism microbiology mutation pathogenicity physiology plant disease plant leaf plant stoma virulence Xanthomonas campestris Arabidopsis Biofilms Glucans Host-Pathogen Interactions Mutation Plant Diseases Plant Leaves Plant Stomata Polysaccharides, Bacterial Pyruvic Acid Virulence Virulence Factors Xanthomonas campestris |
| spellingShingle |
bacterial polysaccharide callose glucan pyruvic acid virulence factor xanthan Arabidopsis biofilm chemistry genetics growth, development and aging host pathogen interaction metabolism microbiology mutation pathogenicity physiology plant disease plant leaf plant stoma virulence Xanthomonas campestris Arabidopsis Biofilms Glucans Host-Pathogen Interactions Mutation Plant Diseases Plant Leaves Plant Stomata Polysaccharides, Bacterial Pyruvic Acid Virulence Virulence Factors Xanthomonas campestris Bianco, M.I. Toum, L. Yaryura, P.M. Mielnichuk, N. Gudesblat, G.E. Roeschlin, R. Marano, M.R. Ielpi, L. Vojnov, A.A. Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris |
| topic_facet |
bacterial polysaccharide callose glucan pyruvic acid virulence factor xanthan Arabidopsis biofilm chemistry genetics growth, development and aging host pathogen interaction metabolism microbiology mutation pathogenicity physiology plant disease plant leaf plant stoma virulence Xanthomonas campestris Arabidopsis Biofilms Glucans Host-Pathogen Interactions Mutation Plant Diseases Plant Leaves Plant Stomata Polysaccharides, Bacterial Pyruvic Acid Virulence Virulence Factors Xanthomonas campestris |
| description |
Xanthan, the main exopolysaccharide (EPS) synthesized by Xanthomonas spp., contributes to bacterial stress tolerance and enhances attachment to plant surfaces by helping in biofilm formation. Therefore, xanthan is essential for successful colonization and growth in planta and has also been proposed to be involved in the promotion of pathogenesis by calcium ion chelation and, hence, in the suppression of the plant defense responses in which this cation acts as a signal. The aim of this work was to study the relationship between xanthan structure and its role as a virulence factor. We analyzed four Xanthomonas campestris pv. campestris mutants that synthesize structural variants of xanthan. We found that the lack of acetyl groups that decorate the internal mannose residues, ketal-pyruvate groups, and external mannose residues affects bacterial adhesion and biofilm architecture. In addition, the mutants that synthesized EPS without pyruvilation or without the external mannose residues did not develop disease symptoms in Arabidopsis thaliana. We also observed that the presence of the external mannose residues and, hence, pyruvilation is required for xanthan to suppress callose deposition as well as to interfere with stomatal defense. In conclusion, pyruvilation of xanthan seems to be essential for Xanthomonas campestris pv. campestris virulence. © 2016 The American Phytopathological Society. |
| format |
JOUR |
| author |
Bianco, M.I. Toum, L. Yaryura, P.M. Mielnichuk, N. Gudesblat, G.E. Roeschlin, R. Marano, M.R. Ielpi, L. Vojnov, A.A. |
| author_facet |
Bianco, M.I. Toum, L. Yaryura, P.M. Mielnichuk, N. Gudesblat, G.E. Roeschlin, R. Marano, M.R. Ielpi, L. Vojnov, A.A. |
| author_sort |
Bianco, M.I. |
| title |
Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris |
| title_short |
Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris |
| title_full |
Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris |
| title_fullStr |
Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris |
| title_full_unstemmed |
Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris |
| title_sort |
xanthan pyruvilation is essential for the virulence of xanthomonas campestris pv. campestris |
| url |
http://hdl.handle.net/20.500.12110/paper_08940282_v29_n9_p688_Bianco |
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