Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus
Tobacco plants were transformed with the open reading frame 3 gene from Potato virus X (PVX) coding for the p12 protein. Although the transgenic plants exhibited a normal morphological aspect, microscopic examination revealed extensive alterations in leaf tissue structure. After being challenged wit...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_08940282_v14_n11_p1274_Kobayashi |
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todo:paper_08940282_v14_n11_p1274_Kobayashi2023-10-03T15:41:54Z Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus Kobayashi, K. Cabral, S. Calamante, G. Maldonado, S. Mentaberry, A. Necrotic lesions Potexvirus Triple gene block Virus movement disease resistance hypersensitivity reaction inoculation leaf tissue open reading frame Potato virus X potato virus Y systemic acquired resistance tobacco Tobacco mosaic virus transgenic plant virus protein virus resistance Embryophyta Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potato virus Y Potexvirus Solanum tuberosum Tobacco mosaic virus Tobacco plants were transformed with the open reading frame 3 gene from Potato virus X (PVX) coding for the p12 protein. Although the transgenic plants exhibited a normal morphological aspect, microscopic examination revealed extensive alterations in leaf tissue structure. After being challenged with PVX, the transgenic plants showed resistance to PVX infection and formation of specific leaf symptoms consisting of concentric rings encircled by necrotic borders. These novel symptoms were accompanied by biochemical changes normally associated with the hypersensitive response (HR) and were absent in noninfected transgenic plants or in PVX-infected nontransgenic plants. No equivalent virus resistance was observed after inoculation with Tobacco mosaic virus or Potato virus Y, suggesting the presence of a specific resistance mechanism. Despite development of HR-like symptoms, systemic acquired resistance was not induced in PVX-infected p12 transgenic plants. No evidence of an RNA-mediated resistance mechanism was found. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08940282_v14_n11_p1274_Kobayashi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Necrotic lesions Potexvirus Triple gene block Virus movement disease resistance hypersensitivity reaction inoculation leaf tissue open reading frame Potato virus X potato virus Y systemic acquired resistance tobacco Tobacco mosaic virus transgenic plant virus protein virus resistance Embryophyta Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potato virus Y Potexvirus Solanum tuberosum Tobacco mosaic virus |
| spellingShingle |
Necrotic lesions Potexvirus Triple gene block Virus movement disease resistance hypersensitivity reaction inoculation leaf tissue open reading frame Potato virus X potato virus Y systemic acquired resistance tobacco Tobacco mosaic virus transgenic plant virus protein virus resistance Embryophyta Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potato virus Y Potexvirus Solanum tuberosum Tobacco mosaic virus Kobayashi, K. Cabral, S. Calamante, G. Maldonado, S. Mentaberry, A. Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus |
| topic_facet |
Necrotic lesions Potexvirus Triple gene block Virus movement disease resistance hypersensitivity reaction inoculation leaf tissue open reading frame Potato virus X potato virus Y systemic acquired resistance tobacco Tobacco mosaic virus transgenic plant virus protein virus resistance Embryophyta Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potato virus Y Potexvirus Solanum tuberosum Tobacco mosaic virus |
| description |
Tobacco plants were transformed with the open reading frame 3 gene from Potato virus X (PVX) coding for the p12 protein. Although the transgenic plants exhibited a normal morphological aspect, microscopic examination revealed extensive alterations in leaf tissue structure. After being challenged with PVX, the transgenic plants showed resistance to PVX infection and formation of specific leaf symptoms consisting of concentric rings encircled by necrotic borders. These novel symptoms were accompanied by biochemical changes normally associated with the hypersensitive response (HR) and were absent in noninfected transgenic plants or in PVX-infected nontransgenic plants. No equivalent virus resistance was observed after inoculation with Tobacco mosaic virus or Potato virus Y, suggesting the presence of a specific resistance mechanism. Despite development of HR-like symptoms, systemic acquired resistance was not induced in PVX-infected p12 transgenic plants. No evidence of an RNA-mediated resistance mechanism was found. |
| format |
JOUR |
| author |
Kobayashi, K. Cabral, S. Calamante, G. Maldonado, S. Mentaberry, A. |
| author_facet |
Kobayashi, K. Cabral, S. Calamante, G. Maldonado, S. Mentaberry, A. |
| author_sort |
Kobayashi, K. |
| title |
Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus |
| title_short |
Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus |
| title_full |
Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus |
| title_fullStr |
Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus |
| title_full_unstemmed |
Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus |
| title_sort |
transgenic tobacco plants expressing the potato virus x open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus |
| url |
http://hdl.handle.net/20.500.12110/paper_08940282_v14_n11_p1274_Kobayashi |
| work_keys_str_mv |
AT kobayashik transgenictobaccoplantsexpressingthepotatovirusxopenreadingframe3genedevelopspecificresistanceandnecroticringsymptomsafterinfectionwiththehomologousvirus AT cabrals transgenictobaccoplantsexpressingthepotatovirusxopenreadingframe3genedevelopspecificresistanceandnecroticringsymptomsafterinfectionwiththehomologousvirus AT calamanteg transgenictobaccoplantsexpressingthepotatovirusxopenreadingframe3genedevelopspecificresistanceandnecroticringsymptomsafterinfectionwiththehomologousvirus AT maldonados transgenictobaccoplantsexpressingthepotatovirusxopenreadingframe3genedevelopspecificresistanceandnecroticringsymptomsafterinfectionwiththehomologousvirus AT mentaberrya transgenictobaccoplantsexpressingthepotatovirusxopenreadingframe3genedevelopspecificresistanceandnecroticringsymptomsafterinfectionwiththehomologousvirus |
| _version_ |
1782029591947247616 |