Role of Melanin as a Scavenger of Active Oxygen Species
The protective role of melanin, either synthetic or derived from a metastatic lung melanoma nodule, was studied in terms of its ability to interact with active oxygen species (O2, H2O2, RO, ROO, etc.). Both melanins showed the ability to react with O2. The superoxide dismutase‐like activity correspo...
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todo:paper_08935785_v6_n5_p348_BUSTAMANTE2023-10-03T15:41:47Z Role of Melanin as a Scavenger of Active Oxygen Species BUSTAMANTE, J. BREDESTON, L. MALANGA, G. MORDOH, J. Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured The protective role of melanin, either synthetic or derived from a metastatic lung melanoma nodule, was studied in terms of its ability to interact with active oxygen species (O2, H2O2, RO, ROO, etc.). Both melanins showed the ability to react with O2. The superoxide dismutase‐like activity corresponds to 21 and 10 U/mg for synthetic and tumor melanin, respectively. The latter value accounts for about 8% of the superoxide dismutase activity of cultured melanoma cells. Neither type of melanin showed catalase‐like or glutathione peroxidase‐like activity. Both types of melanin reacted with RO and ROO radicals as determined by inhibition of the lipid peroxidation reaction of rat liver homogenates. The spontaneous lipid peroxidation of rat liver homogenate was inhibited up to 90% and 80% by synthetic and tumor melanin with half‐maximal effects at 2.5 and 5.5 μg melanin/ml, respectively. The 2,2‐azobis‐(2 amidino propane) (AAPH)‐initiated lipid peroxidation of rat liver homogenate was inhibited up to 3% and 20% by synthetic and tumor melanin, with half maximal effect at 120 and 500 μg melanin/ml, respectively. Both types of melanin were able to protect the in vitro inactivation of glucose oxidase, which occurs in the presence of AAPH‐generated radicals. Copyright © 1993, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08935785_v6_n5_p348_BUSTAMANTE |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured |
| spellingShingle |
Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured BUSTAMANTE, J. BREDESTON, L. MALANGA, G. MORDOH, J. Role of Melanin as a Scavenger of Active Oxygen Species |
| topic_facet |
Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured |
| description |
The protective role of melanin, either synthetic or derived from a metastatic lung melanoma nodule, was studied in terms of its ability to interact with active oxygen species (O2, H2O2, RO, ROO, etc.). Both melanins showed the ability to react with O2. The superoxide dismutase‐like activity corresponds to 21 and 10 U/mg for synthetic and tumor melanin, respectively. The latter value accounts for about 8% of the superoxide dismutase activity of cultured melanoma cells. Neither type of melanin showed catalase‐like or glutathione peroxidase‐like activity. Both types of melanin reacted with RO and ROO radicals as determined by inhibition of the lipid peroxidation reaction of rat liver homogenates. The spontaneous lipid peroxidation of rat liver homogenate was inhibited up to 90% and 80% by synthetic and tumor melanin with half‐maximal effects at 2.5 and 5.5 μg melanin/ml, respectively. The 2,2‐azobis‐(2 amidino propane) (AAPH)‐initiated lipid peroxidation of rat liver homogenate was inhibited up to 3% and 20% by synthetic and tumor melanin, with half maximal effect at 120 and 500 μg melanin/ml, respectively. Both types of melanin were able to protect the in vitro inactivation of glucose oxidase, which occurs in the presence of AAPH‐generated radicals. Copyright © 1993, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
BUSTAMANTE, J. BREDESTON, L. MALANGA, G. MORDOH, J. |
| author_facet |
BUSTAMANTE, J. BREDESTON, L. MALANGA, G. MORDOH, J. |
| author_sort |
BUSTAMANTE, J. |
| title |
Role of Melanin as a Scavenger of Active Oxygen Species |
| title_short |
Role of Melanin as a Scavenger of Active Oxygen Species |
| title_full |
Role of Melanin as a Scavenger of Active Oxygen Species |
| title_fullStr |
Role of Melanin as a Scavenger of Active Oxygen Species |
| title_full_unstemmed |
Role of Melanin as a Scavenger of Active Oxygen Species |
| title_sort |
role of melanin as a scavenger of active oxygen species |
| url |
http://hdl.handle.net/20.500.12110/paper_08935785_v6_n5_p348_BUSTAMANTE |
| work_keys_str_mv |
AT bustamantej roleofmelaninasascavengerofactiveoxygenspecies AT bredestonl roleofmelaninasascavengerofactiveoxygenspecies AT malangag roleofmelaninasascavengerofactiveoxygenspecies AT mordohj roleofmelaninasascavengerofactiveoxygenspecies |
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1807322830628454400 |