Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8
Angiogenesis, the growth OF new capillaries from preexisting blood vessels, is a complex process involving endothelial cell (EC) activation, disruption of vascular basement membranes, and migration and proliferation of ECs. Glycan-mediated recognition has been proposed to play an instrumental role i...
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todo:paper_08926638_v25_n1_p242_CardenasDelgado2023-10-03T15:41:36Z Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 Cárdenas Delgado, V.M. Nugnes, L.G. Colombo, L.L. Troncoso, M.F. Fernández, M.M. Malchiodi, E.L. Frahm, I. Croci, D.O. Compagno, D. Rabinovich, G.A. Wolfenstein-Todel, C. Elola, M.T. Capillary formation Glycan-binding proteins Invasion activated leukocyte cell adhesion molecule galectin galectin 1 galectin 3 galectin 8 vasculotropin angiogenesis animal cell animal experiment article cell invasion cell migration cell nucleus controlled study cytoplasm endothelium cell female human human cell modulation mouse nonhuman priority journal protein binding protein determination protein expression tandem repeat Activated-Leukocyte Cell Adhesion Molecule Animals Antigens, CD31 Cell Line Cell Movement Cell Nucleus Collagen Cytoplasm Dose-Response Relationship, Drug Drug Combinations Endothelial Cells Female Galectins Humans Immunoblotting Immunohistochemistry Immunoprecipitation Kinetics Laminin Mice Mice, Inbred BALB C Neoplasms Neovascularization, Physiologic Protein Binding Proteoglycans Rats Recombinant Proteins Vascular Endothelial Growth Factor A Mus Angiogenesis, the growth OF new capillaries from preexisting blood vessels, is a complex process involving endothelial cell (EC) activation, disruption of vascular basement membranes, and migration and proliferation of ECs. Glycan-mediated recognition has been proposed to play an instrumental role in mediating cell-cell and cell-matrix interactions. Galectins (Gal), a family of glycan-binding proteins with affinity for β-galactosides and a conserved sequence motif, can decipher glycan-containing information and mediate cell-cell communication. Galectin-8 (Gal-8), a member of this family, is a bivalent "tandem-repeat"-type galectin, which possesses 2 CRDs connected by a linker peptide. Here, we show that Gal-8 is endowed with proangiogeneic properties. Functional assays revealed a critical role for this lectin in the regulation of capillary-tube formation and EC migration. Moreover, Matrigel, either supplemented with Gal-8 or vascular endothelial growth factor (VEGF), injected in mice resulted in induction of in vivo angiogenesis. Remarkably, Gal-8 was expressed both in the cytoplasm and nucleus in ECs of normal and tumor vessels. Furthermore, CD166 [activated leukocyte cell adhesion molecule (ALCAM)] was identified as a specific Gal-8-binding partner in normal vascular ECs. Collectively, these data provide the first evidence demonstrating an essential role for Gal-8 in the regulation of angiogenesis with critical implications in tumor biology. © FASEB. Fil:Troncoso, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Croci, D.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Elola, M.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08926638_v25_n1_p242_CardenasDelgado |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Capillary formation Glycan-binding proteins Invasion activated leukocyte cell adhesion molecule galectin galectin 1 galectin 3 galectin 8 vasculotropin angiogenesis animal cell animal experiment article cell invasion cell migration cell nucleus controlled study cytoplasm endothelium cell female human human cell modulation mouse nonhuman priority journal protein binding protein determination protein expression tandem repeat Activated-Leukocyte Cell Adhesion Molecule Animals Antigens, CD31 Cell Line Cell Movement Cell Nucleus Collagen Cytoplasm Dose-Response Relationship, Drug Drug Combinations Endothelial Cells Female Galectins Humans Immunoblotting Immunohistochemistry Immunoprecipitation Kinetics Laminin Mice Mice, Inbred BALB C Neoplasms Neovascularization, Physiologic Protein Binding Proteoglycans Rats Recombinant Proteins Vascular Endothelial Growth Factor A Mus |
| spellingShingle |
Capillary formation Glycan-binding proteins Invasion activated leukocyte cell adhesion molecule galectin galectin 1 galectin 3 galectin 8 vasculotropin angiogenesis animal cell animal experiment article cell invasion cell migration cell nucleus controlled study cytoplasm endothelium cell female human human cell modulation mouse nonhuman priority journal protein binding protein determination protein expression tandem repeat Activated-Leukocyte Cell Adhesion Molecule Animals Antigens, CD31 Cell Line Cell Movement Cell Nucleus Collagen Cytoplasm Dose-Response Relationship, Drug Drug Combinations Endothelial Cells Female Galectins Humans Immunoblotting Immunohistochemistry Immunoprecipitation Kinetics Laminin Mice Mice, Inbred BALB C Neoplasms Neovascularization, Physiologic Protein Binding Proteoglycans Rats Recombinant Proteins Vascular Endothelial Growth Factor A Mus Cárdenas Delgado, V.M. Nugnes, L.G. Colombo, L.L. Troncoso, M.F. Fernández, M.M. Malchiodi, E.L. Frahm, I. Croci, D.O. Compagno, D. Rabinovich, G.A. Wolfenstein-Todel, C. Elola, M.T. Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 |
| topic_facet |
Capillary formation Glycan-binding proteins Invasion activated leukocyte cell adhesion molecule galectin galectin 1 galectin 3 galectin 8 vasculotropin angiogenesis animal cell animal experiment article cell invasion cell migration cell nucleus controlled study cytoplasm endothelium cell female human human cell modulation mouse nonhuman priority journal protein binding protein determination protein expression tandem repeat Activated-Leukocyte Cell Adhesion Molecule Animals Antigens, CD31 Cell Line Cell Movement Cell Nucleus Collagen Cytoplasm Dose-Response Relationship, Drug Drug Combinations Endothelial Cells Female Galectins Humans Immunoblotting Immunohistochemistry Immunoprecipitation Kinetics Laminin Mice Mice, Inbred BALB C Neoplasms Neovascularization, Physiologic Protein Binding Proteoglycans Rats Recombinant Proteins Vascular Endothelial Growth Factor A Mus |
| description |
Angiogenesis, the growth OF new capillaries from preexisting blood vessels, is a complex process involving endothelial cell (EC) activation, disruption of vascular basement membranes, and migration and proliferation of ECs. Glycan-mediated recognition has been proposed to play an instrumental role in mediating cell-cell and cell-matrix interactions. Galectins (Gal), a family of glycan-binding proteins with affinity for β-galactosides and a conserved sequence motif, can decipher glycan-containing information and mediate cell-cell communication. Galectin-8 (Gal-8), a member of this family, is a bivalent "tandem-repeat"-type galectin, which possesses 2 CRDs connected by a linker peptide. Here, we show that Gal-8 is endowed with proangiogeneic properties. Functional assays revealed a critical role for this lectin in the regulation of capillary-tube formation and EC migration. Moreover, Matrigel, either supplemented with Gal-8 or vascular endothelial growth factor (VEGF), injected in mice resulted in induction of in vivo angiogenesis. Remarkably, Gal-8 was expressed both in the cytoplasm and nucleus in ECs of normal and tumor vessels. Furthermore, CD166 [activated leukocyte cell adhesion molecule (ALCAM)] was identified as a specific Gal-8-binding partner in normal vascular ECs. Collectively, these data provide the first evidence demonstrating an essential role for Gal-8 in the regulation of angiogenesis with critical implications in tumor biology. © FASEB. |
| format |
JOUR |
| author |
Cárdenas Delgado, V.M. Nugnes, L.G. Colombo, L.L. Troncoso, M.F. Fernández, M.M. Malchiodi, E.L. Frahm, I. Croci, D.O. Compagno, D. Rabinovich, G.A. Wolfenstein-Todel, C. Elola, M.T. |
| author_facet |
Cárdenas Delgado, V.M. Nugnes, L.G. Colombo, L.L. Troncoso, M.F. Fernández, M.M. Malchiodi, E.L. Frahm, I. Croci, D.O. Compagno, D. Rabinovich, G.A. Wolfenstein-Todel, C. Elola, M.T. |
| author_sort |
Cárdenas Delgado, V.M. |
| title |
Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 |
| title_short |
Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 |
| title_full |
Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 |
| title_fullStr |
Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 |
| title_full_unstemmed |
Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 |
| title_sort |
modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8 |
| url |
http://hdl.handle.net/20.500.12110/paper_08926638_v25_n1_p242_CardenasDelgado |
| work_keys_str_mv |
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1807314781564043264 |