Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1

Peripheral inflammation, both during the prenatal period and in adulthood, impairs adult neurogenesis. We hypothesized that, similar to other programming effects of prenatal treatments, only prenatal inflammation causes long-term consequences in adult neurogenesis and its neurogenic niche. To test t...

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Autores principales:	Graciarena, M., Roca, V., Mathieu, P., Depino, A.M., Pitossi, F.J.
Formato:	JOUR
Materias:	Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 lipopolysaccharide messenger RNA Smad2 protein Smad3 protein sodium chloride transforming growth factor beta1 animal cell animal experiment animal tissue article cell activation central nervous system controlled study dentate gyrus evoked response female gestational age inflammation intracellular signaling male microglia nerve cell culture nervous system development neural stem cell neurophysiology nonhuman prenatal development priority journal protein function rat stem cell niche subventricular zone Age Factors Animals Astrocytes Dentate Gyrus Female Lipopolysaccharides Male Microglia Neurogenesis Pregnancy Rats Rats, Wistar Time Factors Transforming Growth Factor beta1
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_08891591_v34_n_p17_Graciarena
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_08891591_v34_n_p17_Graciarena2023-10-03T15:41:12Z Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1 Graciarena, M. Roca, V. Mathieu, P. Depino, A.M. Pitossi, F.J. Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 lipopolysaccharide messenger RNA Smad2 protein Smad3 protein sodium chloride transforming growth factor beta1 animal cell animal experiment animal tissue article cell activation central nervous system controlled study dentate gyrus evoked response female gestational age inflammation intracellular signaling male microglia nerve cell culture nervous system development neural stem cell neurophysiology nonhuman prenatal development priority journal protein function rat stem cell niche subventricular zone Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 Age Factors Animals Astrocytes Dentate Gyrus Female Inflammation Lipopolysaccharides Male Microglia Neurogenesis Pregnancy Rats Rats, Wistar Time Factors Transforming Growth Factor beta1 Peripheral inflammation, both during the prenatal period and in adulthood, impairs adult neurogenesis. We hypothesized that, similar to other programming effects of prenatal treatments, only prenatal inflammation causes long-term consequences in adult neurogenesis and its neurogenic niche. To test this, pregnant Wistar rats were subcutaneously injected with lipopolysaccharide (LPS; 0.5. mg/kg) or saline solution every other day from gestational/embryonic day (GD) 14-20. In addition adult animals were injected with a single intraperitoneal saline or LPS injection (1. mg/kg) and the effects on neurogenesis were assessed 7. days later. Alternatively, to evaluate long-term consequences of adult LPS injections, LPS (1. mg/kg) was administered peripherally to adult rats four times every other day, and the effects on neurogenesis were assessed 60. days later.Prenatal and adult LPS treatments reduced adult neurogenesis and provoked specific microglial (but not astroglial) activation in the dentate gyrus (DG). However, only prenatal inflammation-mediated effects were long-lasting (at least 60. days). Moreover, these effects were specific to the DG since the Subventricular Zone (SVZ) and the Rostral Migratory Stream (RMS) were not affected. In addition, these stimuli caused differential effects on the molecular components of the neurogenic niche; only prenatal LPS treatment reduced the local levels of TGF-β1 mRNA in the DG. Finally, TGF-β1 exerted its pro-neurogenic effects via the Smad2/3 pathway in a neural stem cell culture.Taken together, these data add evidence to the duration, regional specificity and dramatic consequences of prenatal immune programming on CNS physiology, compared with the limited response observed in the adult brain. © 2013 Elsevier Inc. Fil:Graciarena, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roca, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Depino, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08891591_v34_n_p17_Graciarena
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 lipopolysaccharide messenger RNA Smad2 protein Smad3 protein sodium chloride transforming growth factor beta1 animal cell animal experiment animal tissue article cell activation central nervous system controlled study dentate gyrus evoked response female gestational age inflammation intracellular signaling male microglia nerve cell culture nervous system development neural stem cell neurophysiology nonhuman prenatal development priority journal protein function rat stem cell niche subventricular zone Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 Age Factors Animals Astrocytes Dentate Gyrus Female Inflammation Lipopolysaccharides Male Microglia Neurogenesis Pregnancy Rats Rats, Wistar Time Factors Transforming Growth Factor beta1
spellingShingle	Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 lipopolysaccharide messenger RNA Smad2 protein Smad3 protein sodium chloride transforming growth factor beta1 animal cell animal experiment animal tissue article cell activation central nervous system controlled study dentate gyrus evoked response female gestational age inflammation intracellular signaling male microglia nerve cell culture nervous system development neural stem cell neurophysiology nonhuman prenatal development priority journal protein function rat stem cell niche subventricular zone Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 Age Factors Animals Astrocytes Dentate Gyrus Female Inflammation Lipopolysaccharides Male Microglia Neurogenesis Pregnancy Rats Rats, Wistar Time Factors Transforming Growth Factor beta1 Graciarena, M. Roca, V. Mathieu, P. Depino, A.M. Pitossi, F.J. Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1
topic_facet	Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 lipopolysaccharide messenger RNA Smad2 protein Smad3 protein sodium chloride transforming growth factor beta1 animal cell animal experiment animal tissue article cell activation central nervous system controlled study dentate gyrus evoked response female gestational age inflammation intracellular signaling male microglia nerve cell culture nervous system development neural stem cell neurophysiology nonhuman prenatal development priority journal protein function rat stem cell niche subventricular zone Adult neurogenesis Inflammation Microglial activation Neurogenic niche Prenatal programming TGF-β1 Age Factors Animals Astrocytes Dentate Gyrus Female Inflammation Lipopolysaccharides Male Microglia Neurogenesis Pregnancy Rats Rats, Wistar Time Factors Transforming Growth Factor beta1
description	Peripheral inflammation, both during the prenatal period and in adulthood, impairs adult neurogenesis. We hypothesized that, similar to other programming effects of prenatal treatments, only prenatal inflammation causes long-term consequences in adult neurogenesis and its neurogenic niche. To test this, pregnant Wistar rats were subcutaneously injected with lipopolysaccharide (LPS; 0.5. mg/kg) or saline solution every other day from gestational/embryonic day (GD) 14-20. In addition adult animals were injected with a single intraperitoneal saline or LPS injection (1. mg/kg) and the effects on neurogenesis were assessed 7. days later. Alternatively, to evaluate long-term consequences of adult LPS injections, LPS (1. mg/kg) was administered peripherally to adult rats four times every other day, and the effects on neurogenesis were assessed 60. days later.Prenatal and adult LPS treatments reduced adult neurogenesis and provoked specific microglial (but not astroglial) activation in the dentate gyrus (DG). However, only prenatal inflammation-mediated effects were long-lasting (at least 60. days). Moreover, these effects were specific to the DG since the Subventricular Zone (SVZ) and the Rostral Migratory Stream (RMS) were not affected. In addition, these stimuli caused differential effects on the molecular components of the neurogenic niche; only prenatal LPS treatment reduced the local levels of TGF-β1 mRNA in the DG. Finally, TGF-β1 exerted its pro-neurogenic effects via the Smad2/3 pathway in a neural stem cell culture.Taken together, these data add evidence to the duration, regional specificity and dramatic consequences of prenatal immune programming on CNS physiology, compared with the limited response observed in the adult brain. © 2013 Elsevier Inc.
format	JOUR
author	Graciarena, M. Roca, V. Mathieu, P. Depino, A.M. Pitossi, F.J.
author_facet	Graciarena, M. Roca, V. Mathieu, P. Depino, A.M. Pitossi, F.J.
author_sort	Graciarena, M.
title	Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1
title_short	Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1
title_full	Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1
title_fullStr	Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1
title_full_unstemmed	Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1
title_sort	differential vulnerability of adult neurogenesis by adult and prenatal inflammation: role of tgf-β1
url	http://hdl.handle.net/20.500.12110/paper_08891591_v34_n_p17_Graciarena
work_keys_str_mv	AT graciarenam differentialvulnerabilityofadultneurogenesisbyadultandprenatalinflammationroleoftgfb1 AT rocav differentialvulnerabilityofadultneurogenesisbyadultandprenatalinflammationroleoftgfb1 AT mathieup differentialvulnerabilityofadultneurogenesisbyadultandprenatalinflammationroleoftgfb1 AT depinoam differentialvulnerabilityofadultneurogenesisbyadultandprenatalinflammationroleoftgfb1 AT pitossifj differentialvulnerabilityofadultneurogenesisbyadultandprenatalinflammationroleoftgfb1
_version_	1807322945319600128

Differential vulnerability of adult neurogenesis by adult and prenatal inflammation: Role of TGF-β1

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