Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences
In the present work we report new tools for the characterization of the complete chromosome complement of sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences with similarity to retrotransposons and a homologous rDNA sequence isolated...
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todo:paper_08312796_v53_n3_p172_Talia2023-10-03T15:40:15Z Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences Talia, P. Greizerstein, E. Díaz Quijano, C. Peluffo, L. Fernández, L. Fernández, P. Hopp, H.E. Paniego, N. Heinz, R.A. Poggio, L. BAC-FISH Helianthus annuus Karyotype rDNA Repetitive elements Sunflower Bacteria (microorganisms) Helianthus Helianthus annuus In the present work we report new tools for the characterization of the complete chromosome complement of sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pat-tern suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of specific markers and (or) genes associated with important agricultural traits represents an important tool for future locus-specific identification and physical mapping. Fil:Talia, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Greizerstein, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Peluffo, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernández, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08312796_v53_n3_p172_Talia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
BAC-FISH Helianthus annuus Karyotype rDNA Repetitive elements Sunflower Bacteria (microorganisms) Helianthus Helianthus annuus |
| spellingShingle |
BAC-FISH Helianthus annuus Karyotype rDNA Repetitive elements Sunflower Bacteria (microorganisms) Helianthus Helianthus annuus Talia, P. Greizerstein, E. Díaz Quijano, C. Peluffo, L. Fernández, L. Fernández, P. Hopp, H.E. Paniego, N. Heinz, R.A. Poggio, L. Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences |
| topic_facet |
BAC-FISH Helianthus annuus Karyotype rDNA Repetitive elements Sunflower Bacteria (microorganisms) Helianthus Helianthus annuus |
| description |
In the present work we report new tools for the characterization of the complete chromosome complement of sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pat-tern suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of specific markers and (or) genes associated with important agricultural traits represents an important tool for future locus-specific identification and physical mapping. |
| format |
JOUR |
| author |
Talia, P. Greizerstein, E. Díaz Quijano, C. Peluffo, L. Fernández, L. Fernández, P. Hopp, H.E. Paniego, N. Heinz, R.A. Poggio, L. |
| author_facet |
Talia, P. Greizerstein, E. Díaz Quijano, C. Peluffo, L. Fernández, L. Fernández, P. Hopp, H.E. Paniego, N. Heinz, R.A. Poggio, L. |
| author_sort |
Talia, P. |
| title |
Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences |
| title_short |
Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences |
| title_full |
Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences |
| title_fullStr |
Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences |
| title_full_unstemmed |
Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences |
| title_sort |
cytological characterization of sunflower by in situ hybridization using homologous rdna sequences and a bac clone containing highly represented repetitive retrotransposon-like sequences |
| url |
http://hdl.handle.net/20.500.12110/paper_08312796_v53_n3_p172_Talia |
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1782029492833746944 |