Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe

RFLP analysis of mtDNA in natural populations is a valuable tool for phylogeographic and population genetic studies. The amplification of long DNA fragments using universal primers may contribute to the development of novel homologous probes in species for which no previous genomic information is av...

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Autores principales: Pensel, S.M., Vilardi, J.C., Remis, M.I.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_08312796_v48_n6_p971_Pensel
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spelling todo:paper_08312796_v48_n6_p971_Pensel2023-10-03T15:40:15Z Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe Pensel, S.M. Vilardi, J.C. Remis, M.I. Grasshopper Long-PCR Mitochondrial DNA RFLP Universal primers mitochondrial DNA animal article DNA probe genetic variability genetics grasshopper mitochondrion nucleotide sequence polymerase chain reaction restriction fragment length polymorphism sequence homology Animals Conserved Sequence DNA Probes DNA, Mitochondrial Genetic Variation Grasshoppers Mitochondria Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid Variation (Genetics) Orthoptera Sinipta dalmani RFLP analysis of mtDNA in natural populations is a valuable tool for phylogeographic and population genetic studies. The amplification of long DNA fragments using universal primers may contribute to the development of novel homologous probes in species for which no previous genomic information is available. Here we report how we obtained the complete mtDNA genome of Sinipta dalmani (Orthoptera) in 2 fragments (7 and 9 kb) using primers of conserved regions. The specificity of the PCR reactions was ultimately confirmed by several lines of evidence. These fragments were used as a probe for a mtDNA RFLP study in S. dalmani that analyzed the pattern of haplotype distribution and nucleotide diversity within and among chromosomally differentiated natural populations. Our results suggest that the restriction in gene flow detected at the molecular level may explain the chromosome differentiation detected previously and the maintenance of chromosome polymorphism in some areas of S. dalmani geographic distribution. © 2005 NRC. Fil:Pensel, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vilardi, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Remis, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08312796_v48_n6_p971_Pensel
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Grasshopper
Long-PCR
Mitochondrial DNA
RFLP
Universal primers
mitochondrial DNA
animal
article
DNA probe
genetic variability
genetics
grasshopper
mitochondrion
nucleotide sequence
polymerase chain reaction
restriction fragment length polymorphism
sequence homology
Animals
Conserved Sequence
DNA Probes
DNA, Mitochondrial
Genetic Variation
Grasshoppers
Mitochondria
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sequence Homology, Nucleic Acid
Variation (Genetics)
Orthoptera
Sinipta dalmani
spellingShingle Grasshopper
Long-PCR
Mitochondrial DNA
RFLP
Universal primers
mitochondrial DNA
animal
article
DNA probe
genetic variability
genetics
grasshopper
mitochondrion
nucleotide sequence
polymerase chain reaction
restriction fragment length polymorphism
sequence homology
Animals
Conserved Sequence
DNA Probes
DNA, Mitochondrial
Genetic Variation
Grasshoppers
Mitochondria
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sequence Homology, Nucleic Acid
Variation (Genetics)
Orthoptera
Sinipta dalmani
Pensel, S.M.
Vilardi, J.C.
Remis, M.I.
Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
topic_facet Grasshopper
Long-PCR
Mitochondrial DNA
RFLP
Universal primers
mitochondrial DNA
animal
article
DNA probe
genetic variability
genetics
grasshopper
mitochondrion
nucleotide sequence
polymerase chain reaction
restriction fragment length polymorphism
sequence homology
Animals
Conserved Sequence
DNA Probes
DNA, Mitochondrial
Genetic Variation
Grasshoppers
Mitochondria
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sequence Homology, Nucleic Acid
Variation (Genetics)
Orthoptera
Sinipta dalmani
description RFLP analysis of mtDNA in natural populations is a valuable tool for phylogeographic and population genetic studies. The amplification of long DNA fragments using universal primers may contribute to the development of novel homologous probes in species for which no previous genomic information is available. Here we report how we obtained the complete mtDNA genome of Sinipta dalmani (Orthoptera) in 2 fragments (7 and 9 kb) using primers of conserved regions. The specificity of the PCR reactions was ultimately confirmed by several lines of evidence. These fragments were used as a probe for a mtDNA RFLP study in S. dalmani that analyzed the pattern of haplotype distribution and nucleotide diversity within and among chromosomally differentiated natural populations. Our results suggest that the restriction in gene flow detected at the molecular level may explain the chromosome differentiation detected previously and the maintenance of chromosome polymorphism in some areas of S. dalmani geographic distribution. © 2005 NRC.
format JOUR
author Pensel, S.M.
Vilardi, J.C.
Remis, M.I.
author_facet Pensel, S.M.
Vilardi, J.C.
Remis, M.I.
author_sort Pensel, S.M.
title Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
title_short Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
title_full Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
title_fullStr Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
title_full_unstemmed Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
title_sort mitochondrial dna variation in the grasshopper sinipta dalmani: application of long-pcr to the development of a homologous probe
url http://hdl.handle.net/20.500.12110/paper_08312796_v48_n6_p971_Pensel
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AT remismi mitochondrialdnavariationinthegrasshoppersiniptadalmaniapplicationoflongpcrtothedevelopmentofahomologousprobe
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