The genomic composition of Tricepiro, a synthetic forage crop
Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid tri...
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todo:paper_08312796_v48_n1_p154_Ferrari2023-10-03T15:40:14Z The genomic composition of Tricepiro, a synthetic forage crop Ferrari, M.R. Greizerstein, E.J. Paccapelo, H.A. Naranjo, C.A. Cuadrado, A. Jouve, N. Poggio, L. FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid triticale (2n = 6x = 42) and octoploid Trigopiro (2n = 8x = 56). Several years of breeding produced a forage crop with valuable characteristics from Secale, Triticum, and Thinopyrum. The aim of this work is to analyse the real genomic constitution of this important synthetic crop. In situ hybridization using total DNA of Secale, Triticum, and Thinopyrum as a probe (GISH) labelled with biotin and (or) digoxigenin showed that tricepiro is composed of 14 rye chromosomes and 28 wheat chromosomes. Small zones of introgression of Thinopyrum on wheat chromosomes were detected. The FISH using the rye repetitive DNA probe pSc 119.2 labelled with biotin let us characterize the seven pairs of rye chromosomes. Moreover, several wheat chromosomes belonging to A and B genomes were distinguished. Therefore, tricepiro is a synthetic hexaploid (2n = 6x = 42) being AABBRR in its genomic composition, with zones of introgression of Thinopyrum in the A genome of wheat. © 2005 NRC Canada. Fil:Ferrari, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Greizerstein, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Naranjo, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08312796_v48_n1_p154_Ferrari |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum |
| spellingShingle |
FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum Ferrari, M.R. Greizerstein, E.J. Paccapelo, H.A. Naranjo, C.A. Cuadrado, A. Jouve, N. Poggio, L. The genomic composition of Tricepiro, a synthetic forage crop |
| topic_facet |
FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum |
| description |
Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid triticale (2n = 6x = 42) and octoploid Trigopiro (2n = 8x = 56). Several years of breeding produced a forage crop with valuable characteristics from Secale, Triticum, and Thinopyrum. The aim of this work is to analyse the real genomic constitution of this important synthetic crop. In situ hybridization using total DNA of Secale, Triticum, and Thinopyrum as a probe (GISH) labelled with biotin and (or) digoxigenin showed that tricepiro is composed of 14 rye chromosomes and 28 wheat chromosomes. Small zones of introgression of Thinopyrum on wheat chromosomes were detected. The FISH using the rye repetitive DNA probe pSc 119.2 labelled with biotin let us characterize the seven pairs of rye chromosomes. Moreover, several wheat chromosomes belonging to A and B genomes were distinguished. Therefore, tricepiro is a synthetic hexaploid (2n = 6x = 42) being AABBRR in its genomic composition, with zones of introgression of Thinopyrum in the A genome of wheat. © 2005 NRC Canada. |
| format |
JOUR |
| author |
Ferrari, M.R. Greizerstein, E.J. Paccapelo, H.A. Naranjo, C.A. Cuadrado, A. Jouve, N. Poggio, L. |
| author_facet |
Ferrari, M.R. Greizerstein, E.J. Paccapelo, H.A. Naranjo, C.A. Cuadrado, A. Jouve, N. Poggio, L. |
| author_sort |
Ferrari, M.R. |
| title |
The genomic composition of Tricepiro, a synthetic forage crop |
| title_short |
The genomic composition of Tricepiro, a synthetic forage crop |
| title_full |
The genomic composition of Tricepiro, a synthetic forage crop |
| title_fullStr |
The genomic composition of Tricepiro, a synthetic forage crop |
| title_full_unstemmed |
The genomic composition of Tricepiro, a synthetic forage crop |
| title_sort |
genomic composition of tricepiro, a synthetic forage crop |
| url |
http://hdl.handle.net/20.500.12110/paper_08312796_v48_n1_p154_Ferrari |
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