Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance

The plant vp1 gene encodes a transcription factor originally identified in maize that participates in the control of the transition from embryogenesis to seed germination. Different lines of evidence suggest that vp1 participates in preharvest sprouting (PHS) resistance in cereals, but the genetic r...

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Autores principales: Carrari, F., Benech-Arnold, R., Osuna-Fernandez, R., Hopp, E., Sanchez, R., Iusem, N., Lijavetzky, D.
Formato: JOUR
Materias:
Dormancy
Genetic mapping
Pre-harvest sprouting
Quantitative trait loci (QTL)
Sbvp1
Viviparous-1
Chromosome Mapping
Chromosomes, Plant
Genes, Plant
Genetic Markers
Genome, Plant
Germination
Linkage (Genetics)
Polymorphism, Restriction Fragment Length
Quantitative Trait Loci
Seeds
Sequence Analysis, DNA
Sorghum
Embryophyta
Sorghum bicolor
Zea mays
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_08312796_v46_n2_p253_Carrari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_08312796_v46_n2_p253_Carrari
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spelling todo:paper_08312796_v46_n2_p253_Carrari2023-10-03T15:40:14Z Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance Carrari, F. Benech-Arnold, R. Osuna-Fernandez, R. Hopp, E. Sanchez, R. Iusem, N. Lijavetzky, D. Dormancy Genetic mapping Pre-harvest sprouting Quantitative trait loci (QTL) Sbvp1 Viviparous-1 Chromosome Mapping Chromosomes, Plant Genes, Plant Genetic Markers Genome, Plant Germination Linkage (Genetics) Polymorphism, Restriction Fragment Length Quantitative Trait Loci Seeds Sequence Analysis, DNA Sorghum Embryophyta Sorghum bicolor Zea mays The plant vp1 gene encodes a transcription factor originally identified in maize that participates in the control of the transition from embryogenesis to seed germination. Different lines of evidence suggest that vp1 participates in preharvest sprouting (PHS) resistance in cereals, but the genetic relationship is not yet established. Thus, the aim of this study was to investigate if there is a connection between vp1 and the formerly documented quantitative trait loci for PHS in Sorghum bicolor. This gene was mapped in sorghum using two well-studied inbred lines displaying contrasting PHS phenotypes. Linkage analysis revealed that the S. bicolor vp1 (Sbvp1) locus is linked to markers located on chromosomes 3 and 8 in maize. Analysis indicated that this gene is not correlated with PHS at a statistically significant level. Structural analysis of the Sbvp1 gene from both inbred lines showed a high degree of overall conservation (low polymorphism), even in the promoter region. These findings, taken together with the previously observed correlation between the pattern of expression of this gene and PHS performance, suggest that Sbvp1 might act downstream in the signalling pathway that leads to seed germination, or even be differentially regulated as a consequence rather than as a cause of the phenotypic behaviour. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08312796_v46_n2_p253_Carrari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Dormancy
Genetic mapping
Pre-harvest sprouting
Quantitative trait loci (QTL)
Sbvp1
Viviparous-1
Chromosome Mapping
Chromosomes, Plant
Genes, Plant
Genetic Markers
Genome, Plant
Germination
Linkage (Genetics)
Polymorphism, Restriction Fragment Length
Quantitative Trait Loci
Seeds
Sequence Analysis, DNA
Sorghum
Embryophyta
Sorghum bicolor
Zea mays
spellingShingle Dormancy
Genetic mapping
Pre-harvest sprouting
Quantitative trait loci (QTL)
Sbvp1
Viviparous-1
Chromosome Mapping
Chromosomes, Plant
Genes, Plant
Genetic Markers
Genome, Plant
Germination
Linkage (Genetics)
Polymorphism, Restriction Fragment Length
Quantitative Trait Loci
Seeds
Sequence Analysis, DNA
Sorghum
Embryophyta
Sorghum bicolor
Zea mays
Carrari, F.
Benech-Arnold, R.
Osuna-Fernandez, R.
Hopp, E.
Sanchez, R.
Iusem, N.
Lijavetzky, D.
Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
topic_facet Dormancy
Genetic mapping
Pre-harvest sprouting
Quantitative trait loci (QTL)
Sbvp1
Viviparous-1
Chromosome Mapping
Chromosomes, Plant
Genes, Plant
Genetic Markers
Genome, Plant
Germination
Linkage (Genetics)
Polymorphism, Restriction Fragment Length
Quantitative Trait Loci
Seeds
Sequence Analysis, DNA
Sorghum
Embryophyta
Sorghum bicolor
Zea mays
description The plant vp1 gene encodes a transcription factor originally identified in maize that participates in the control of the transition from embryogenesis to seed germination. Different lines of evidence suggest that vp1 participates in preharvest sprouting (PHS) resistance in cereals, but the genetic relationship is not yet established. Thus, the aim of this study was to investigate if there is a connection between vp1 and the formerly documented quantitative trait loci for PHS in Sorghum bicolor. This gene was mapped in sorghum using two well-studied inbred lines displaying contrasting PHS phenotypes. Linkage analysis revealed that the S. bicolor vp1 (Sbvp1) locus is linked to markers located on chromosomes 3 and 8 in maize. Analysis indicated that this gene is not correlated with PHS at a statistically significant level. Structural analysis of the Sbvp1 gene from both inbred lines showed a high degree of overall conservation (low polymorphism), even in the promoter region. These findings, taken together with the previously observed correlation between the pattern of expression of this gene and PHS performance, suggest that Sbvp1 might act downstream in the signalling pathway that leads to seed germination, or even be differentially regulated as a consequence rather than as a cause of the phenotypic behaviour.
format JOUR
author Carrari, F.
Benech-Arnold, R.
Osuna-Fernandez, R.
Hopp, E.
Sanchez, R.
Iusem, N.
Lijavetzky, D.
author_facet Carrari, F.
Benech-Arnold, R.
Osuna-Fernandez, R.
Hopp, E.
Sanchez, R.
Iusem, N.
Lijavetzky, D.
author_sort Carrari, F.
title Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
title_short Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
title_full Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
title_fullStr Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
title_full_unstemmed Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
title_sort genetic mapping of the sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
url http://hdl.handle.net/20.500.12110/paper_08312796_v46_n2_p253_Carrari
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