Pleiotropic effects of 5-aminolevulinic acid in mouse brain

5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the cen...

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Autores principales:	Lavandera, J., Rodríguez, J., Ruspini, S., Meiss, R., Zuccoli, J.R., Martínez, M.D.C., Gerez, E., Batlle, A., Buzaleh, A.M.
Formato:	JOUR
Materias:	5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins Anti-oxidant response Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase antioxidant heme photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_08298211_v94_n4_p297_Lavandera
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_08298211_v94_n4_p297_Lavandera
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spelling	todo:paper_08298211_v94_n4_p297_Lavandera2023-10-03T15:40:12Z Pleiotropic effects of 5-aminolevulinic acid in mouse brain Lavandera, J. Rodríguez, J. Ruspini, S. Meiss, R. Zuccoli, J.R. Martínez, M.D.C. Gerez, E. Batlle, A. Buzaleh, A.M. 5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents 5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the central nervous system. Heme metabolism, the cholinergic system, the defense enzyme system, and nitric oxide metabolism were evaluated in the encephalon of CF-1 mice receiving a single (40 mg/kg body mass) or multiple doses of ALA (40 mg/kg, every 48 h for 14 days). We subsequently found ALA accumulation in the encephalon of the mice. ALA also altered the brain cholinergic system. After one dose of ALA, a decrease in superoxide dismutase activity and a reduction in glutathione levels were detected, whereas malondialdehyde levels and catalase activity were increased. Heme oxygenase was also increased as an antioxidant response to protect the encephalon against injury. All nitric oxide synthase isoforms were induced by ALA, these changes were more significant for the inducible isoform in glial cells. In conclusion, ALA affected several metabolic pathways in mouse encephalon. Data indicate that a rapid response to oxidative stress was developed; however, with long-term intoxication, the redox balance was probably restored, thereby minimizing oxidative damage. © 2016 Published by NRC Research Press. Fil:Lavandera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gerez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08298211_v94_n4_p297_Lavandera
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents
spellingShingle	5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents Lavandera, J. Rodríguez, J. Ruspini, S. Meiss, R. Zuccoli, J.R. Martínez, M.D.C. Gerez, E. Batlle, A. Buzaleh, A.M. Pleiotropic effects of 5-aminolevulinic acid in mouse brain
topic_facet	5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents
description	5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the central nervous system. Heme metabolism, the cholinergic system, the defense enzyme system, and nitric oxide metabolism were evaluated in the encephalon of CF-1 mice receiving a single (40 mg/kg body mass) or multiple doses of ALA (40 mg/kg, every 48 h for 14 days). We subsequently found ALA accumulation in the encephalon of the mice. ALA also altered the brain cholinergic system. After one dose of ALA, a decrease in superoxide dismutase activity and a reduction in glutathione levels were detected, whereas malondialdehyde levels and catalase activity were increased. Heme oxygenase was also increased as an antioxidant response to protect the encephalon against injury. All nitric oxide synthase isoforms were induced by ALA, these changes were more significant for the inducible isoform in glial cells. In conclusion, ALA affected several metabolic pathways in mouse encephalon. Data indicate that a rapid response to oxidative stress was developed; however, with long-term intoxication, the redox balance was probably restored, thereby minimizing oxidative damage. © 2016 Published by NRC Research Press.
format	JOUR
author	Lavandera, J. Rodríguez, J. Ruspini, S. Meiss, R. Zuccoli, J.R. Martínez, M.D.C. Gerez, E. Batlle, A. Buzaleh, A.M.
author_facet	Lavandera, J. Rodríguez, J. Ruspini, S. Meiss, R. Zuccoli, J.R. Martínez, M.D.C. Gerez, E. Batlle, A. Buzaleh, A.M.
author_sort	Lavandera, J.
title	Pleiotropic effects of 5-aminolevulinic acid in mouse brain
title_short	Pleiotropic effects of 5-aminolevulinic acid in mouse brain
title_full	Pleiotropic effects of 5-aminolevulinic acid in mouse brain
title_fullStr	Pleiotropic effects of 5-aminolevulinic acid in mouse brain
title_full_unstemmed	Pleiotropic effects of 5-aminolevulinic acid in mouse brain
title_sort	pleiotropic effects of 5-aminolevulinic acid in mouse brain
url	http://hdl.handle.net/20.500.12110/paper_08298211_v94_n4_p297_Lavandera
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Pleiotropic effects of 5-aminolevulinic acid in mouse brain

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